{"id":"5b0e6bb5-9d7f-51a9-b578-ce6a1d812e16","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-pn-vitamer-specificity","predicate":"has-context-dependent-effect-on","statement":"Pyridoxal, pyridoxamine, PLP and PMP did not reduce viability in the tested 24-hour cell assay.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"1e66e3e4-fb25-5784-83a9-7221a3919b21","mechanism_event_label":"The tested B6 forms behaved differently in this assay.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"4f4af47e-a4f7-5dca-8712-c96c5e3724f9","slug":"sh-sy5y-viability","display_name":"SH-SY5Y cell viability by MTT","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1e66e3e4-fb25-5784-83a9-7221a3919b21","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-pn-vitamer-specificity-event","event_type":"observed_intervention","label":"The tested B6 forms behaved differently in this assay.","description":"Pyridoxal, pyridoxamine, PLP and PMP did not reduce viability in the tested 24-hour cell assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"comparator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9bf6269d-9e01-553d-bd8b-11a0d613bfca","slug":"pyridoxal","display_name":"Pyridoxal","entity_type_key":"small_molecule"},"role":"comparator","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9c195b42-dc87-51f6-a317-d4585a0e8d84","slug":"pyridoxamine","display_name":"Pyridoxamine","entity_type_key":"small_molecule"},"role":"comparator","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c8dec85c-c85d-5a55-ba85-ad3c1b3e332b","slug":"pyridoxamine-phosphate","display_name":"Pyridoxamine 5-prime-phosphate","entity_type_key":"small_molecule"},"role":"comparator","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4f4af47e-a4f7-5dca-8712-c96c5e3724f9","slug":"sh-sy5y-viability","display_name":"SH-SY5Y cell viability by MTT","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Comparators 0-500 micromolar for 24 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure-class","value_text":"Experimental excess pyridoxine; not dietary B6 deficiency","comparator":null,"unit":null,"notes":"","entity":{"slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"}},{"dimension":"limitations","value_text":"A negative short-term cell assay proves neither universal safety nor equivalent toxicity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The tested B6 forms behaved differently in this assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"SH-SY5Y and CaCo-2 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6480f4bb-4d0b-5e38-a8c3-2e7f52ddb2b9","evidence_kind":"source_excerpt","locator":"Lines 1245-1256","start_line":1245,"end_line":1256,"excerpt":"### b6-neuro-pn-vitamer-specificity\nPyridoxal, pyridoxamine, PLP and PMP did not reduce viability in the tested 24-hour cell assay.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The tested B6 forms behaved differently in this assay.\norganism: Homo sapiens\ntissue_or_cell_type: SH-SY5Y and CaCo-2 cells\nexperimental_model: Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays\nlimitations: A negative short-term cell assay proves neither universal safety nor equivalent toxicity.\nexposure: Comparators 0-500 micromolar for 24 hours.\nexposure-class: Experimental excess pyridoxine; not dietary B6 deficiency\n[vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009","model_system":"Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}