{"id":"191c3793-ab89-5c43-9a84-e8a1f3af55e9","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-pn-cell-viability","predicate":"reduces","statement":"Pyridoxine reduced MTT-measured viability of differentiated SH-SY5Y cells after 24 hours in a concentration-dependent manner.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"cc7d2b57-e10a-57d1-9690-b7401f3fdedf","mechanism_event_label":"Added pyridoxine harmed this experimental neuronal cell model.","subject":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"object":{"id":"4f4af47e-a4f7-5dca-8712-c96c5e3724f9","slug":"sh-sy5y-viability","display_name":"SH-SY5Y cell viability by MTT","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cc7d2b57-e10a-57d1-9690-b7401f3fdedf","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-pn-cell-viability-event","event_type":"observed_intervention","label":"Added pyridoxine harmed this experimental neuronal cell model.","description":"Pyridoxine reduced MTT-measured viability of differentiated SH-SY5Y cells after 24 hours in a concentration-dependent manner.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4f4af47e-a4f7-5dca-8712-c96c5e3724f9","slug":"sh-sy5y-viability","display_name":"SH-SY5Y cell viability by MTT","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Differentiated human SH-SY5Y and CaCo-2 cells; 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topical membership is not evidence of a direct dietary effect.\nplain_language: Added pyridoxine harmed this experimental neuronal cell model.\norganism: Homo sapiens\ntissue_or_cell_type: Differentiated SH-SY5Y neuroblastoma cells\nexperimental_model: Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays\nlimitations: Tumor-derived cells and MTT endpoint; no oral-dose conversion or human toxicity threshold.\nexposure: Pyridoxine 0-5 micromolar for 24 hours.\nexposure-class: Experimental excess pyridoxine; not dietary B6 deficiency\n[vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009","model_system":"Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}