Component
P2X4 receptor channel / P2RX4
ATP-gated cation channel, the most widespread P2X subtype in brain.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Ivermectin was a specific positive allosteric effector of heterologously expressed P2X4 channels with an EC50 near 250 nanomolar, increasing current amplitude and slowing deactivation.
Experimental context and source evidence
- duration
- Acute
- evidence_access
- Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
- experimental_model
- Heterologously expressed P2X4 and P2X4/P2X6 channels
- exposure
- Submicromolar ivermectin, rapid and reversible
- limitations
- The effect was specific to P2X4 and absent at P2X2, P2X3, P2X2/P2X3 and P2X7, so it cannot be generalised to ATP signalling as a whole.
- organism
- Heterologously expressed P2X4 and P2X4/P2X6 channels
- plain_language
- Ivermectin was a specific positive allosteric effector of heterologously expressed P2X4 channels with an EC50 near 250 nanomolar, increasing current amplitude and slowing deactivation.
- primary_references
- Allosteric control of gating and kinetics at P2X(4) receptor channels. (1999). https://pubmed.ncbi.nlm.nih.gov/10460235/ DOI: 10.1523/JNEUROSCI.19-17-07289.1999
- route
- In vitro
- tissue
- ATP-gated cation channel gating and kinetics
Ivermectin: mechanism of action across parasite, host barrier and mammalian targets (2026-09-22) · lines 90–99
Original AI-assisted curation of sixteen primary studies resolved by PubMed title search and cross-checked against live PubMed metadata. Study-specific citations, concentrations, negative findings and limitations retained. Not publisher full text. · supports · · source_derived_draft · unverified_draft
## ivermectin-p2x4-potentiation Ivermectin was a specific positive allosteric effector of heterologously expressed P2X4 channels with an EC50 near 250 nanomolar, increasing current amplitude and slowing deactivation. Model/species: Heterologously expressed P2X4 and P2X4/P2X6 channels Tissue/system: ATP-gated cation channel gating and kinetics Exposure: Submicromolar ivermectin, rapid and reversible Route: In vitro Duration: Acute Limits: The effect was specific to P2X4 and absent at P2X2, P2X3, P2X2/P2X3 and P2X7, so it cannot be generalised to ATP signalling as a whole. Primary reference: Allosteric control of gating and kinetics at P2X(4) receptor channels. (1999). https://pubmed.ncbi.nlm.nih.gov/10460235/ DOI: 10.1523/JNEUROSCI.19-17-07289.1999 Access: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
Complete structured claim and evidence
Where it participates (unsigned role)
Ivermectin produced no significant effect on fast ATP-evoked inward currents in adult trigeminal mesencephalic nucleus or hippocampal CA1 neurons.
Experimental context and source evidence
- duration
- Acute
- evidence_access
- Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
- experimental_model
- Adult rat trigeminal mesencephalic nucleus and hippocampal CA1 neurons
- exposure
- Ivermectin at concentrations that modulated heterologously expressed P2X4
- limitations
- The authors read this as evidence that homomeric P2X4 is not the primary P2X subtype in these neurons, not as evidence that ivermectin fails to reach the channel.
- organism
- Adult rat trigeminal mesencephalic nucleus and hippocampal CA1 neurons
- plain_language
- Ivermectin produced no significant effect on fast ATP-evoked inward currents in adult trigeminal mesencephalic nucleus or hippocampal CA1 neurons.
- primary_references
- Allosteric control of gating and kinetics at P2X(4) receptor channels. (1999). https://pubmed.ncbi.nlm.nih.gov/10460235/ DOI: 10.1523/JNEUROSCI.19-17-07289.1999
- route
- In vitro
- tissue
- Endogenously expressed P2X receptor currents
Ivermectin: mechanism of action across parasite, host barrier and mammalian targets (2026-09-22) · lines 101–110
Original AI-assisted curation of sixteen primary studies resolved by PubMed title search and cross-checked against live PubMed metadata. Study-specific citations, concentrations, negative findings and limitations retained. Not publisher full text. · supports · · source_derived_draft · unverified_draft
## ivermectin-native-p2x-current-null Ivermectin produced no significant effect on fast ATP-evoked inward currents in adult trigeminal mesencephalic nucleus or hippocampal CA1 neurons. Model/species: Adult rat trigeminal mesencephalic nucleus and hippocampal CA1 neurons Tissue/system: Endogenously expressed P2X receptor currents Exposure: Ivermectin at concentrations that modulated heterologously expressed P2X4 Route: In vitro Duration: Acute Limits: The authors read this as evidence that homomeric P2X4 is not the primary P2X subtype in these neurons, not as evidence that ivermectin fails to reach the channel. Primary reference: Allosteric control of gating and kinetics at P2X(4) receptor channels. (1999). https://pubmed.ncbi.nlm.nih.gov/10460235/ DOI: 10.1523/JNEUROSCI.19-17-07289.1999 Access: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.