{"id":"1c6a79df-5fee-53e8-b2a8-265aaf4c2a2b","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-native-p2x-current-null","predicate":"no_detected_effect_on","statement":"Ivermectin produced no significant effect on fast ATP-evoked inward currents in adult trigeminal mesencephalic nucleus or hippocampal CA1 neurons.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"a2e158d3-f582-581a-a643-8a3522f6d232","mechanism_event_label":"Ivermectin produced no significant effect on fast ATP-evoked inward currents in adult trigeminal mesencephalic nucleus or hippocampal CA1 neurons.","subject":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"object":{"id":"2ff406eb-adb6-5908-b1ed-646f630c462f","slug":"native-p2x-current-adult-neurons","display_name":"Native ATP-evoked P2X current in adult neurons","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a2e158d3-f582-581a-a643-8a3522f6d232","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-native-p2x-current-null-event","event_type":"observed_relationship","label":"Ivermectin produced no significant effect on fast ATP-evoked inward currents in adult trigeminal mesencephalic nucleus or hippocampal CA1 neurons.","description":"Ivermectin produced no significant effect on fast ATP-evoked inward currents in adult trigeminal mesencephalic nucleus or hippocampal CA1 neurons.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2ff406eb-adb6-5908-b1ed-646f630c462f","slug":"native-p2x-current-adult-neurons","display_name":"Native ATP-evoked P2X current in adult neurons","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"15b750d6-4e83-5abb-b460-620d238b688c","slug":"p2rx4","display_name":"P2X4 receptor channel / P2RX4","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. 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(1999). https://pubmed.ncbi.nlm.nih.gov/10460235/ DOI: 10.1523/JNEUROSCI.19-17-07289.1999","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Endogenously expressed P2X receptor currents","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a52c189c-23ad-5889-92a3-955cc4fa7b8d","evidence_kind":"source_excerpt","locator":"Lines 101-110","start_line":101,"end_line":110,"excerpt":"## ivermectin-native-p2x-current-null\nIvermectin produced no significant effect on fast ATP-evoked inward currents in adult trigeminal mesencephalic nucleus or hippocampal CA1 neurons.\nModel/species: Adult rat trigeminal mesencephalic nucleus and hippocampal CA1 neurons\nTissue/system: Endogenously expressed P2X receptor currents\nExposure: Ivermectin at concentrations that modulated heterologously expressed P2X4\nRoute: In vitro\nDuration: Acute\nLimits: The authors read this as evidence that homomeric P2X4 is not the primary P2X subtype in these neurons, not as evidence that ivermectin fails to reach the channel.\nPrimary reference: Allosteric control of gating and kinetics at P2X(4) receptor channels. 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