Component
Mucin-type O-linked glycosylation
Mucin-type O-linked glycosylation. Experimental scope belongs to each linked claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
D-galactose partially improved the 24-hour O-glycan lectin readout but failed to rescue the three-day benzyl-GalNAc mass-spectrometry phenotype in TMEM165-knockout HEK cells.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- TMEM165 Golgi cation-homeostasis protein (affected_protein); Manganese(II) ion (effective_comparator)
- evidence_span
- {"source_cache": "artifacts/manganese-glycosylation-sources/morelle2022.txt", "locator": "Results; O-linked lectin and mass-spectrometry endpoints", "file_sha256": "91d05677c6d7bb4224f71742888e39533e3f130d59e6b2ffcae3e2b2c058e9aa", "start_char": 19358, "end_char": 22255, "text_sha256": "e0b72a5427217107308f73e2304f47c761a25448096f516ca75c497eb8e389d5", "text_characters": 2897}
- experimental_model
- Control and TMEM165-knockout HEK293 glycosylation assays
- exposure
- Figure 3 lectin staining: 2.5 micromolar MnCl2 and/or 1 millimolar galactose for 24 hours. Figure 4: same additions with 250 micromolar benzyl-GalNAc for three days.
- limitations
- Cell rescue is not evidence for a safe human dose. N-linked, mucin-type O-linked and proteoglycan GAG endpoints are distinct; restoring one is not proof of global correction.
- nutrient_topic
- Manganese research collection; topical membership is not evidence of a direct dietary effect. · Manganese
- organism
- Homo sapiens
- plain_language
- One O-glycan test improved partly while a different test remained abnormal; galactose did not normalize this pathway.
- primary_references
- [mn-gly-morelle2022] Differential Effects of D-Galactose Supplementation on Golgi Glycosylation Defects in TMEM165 Deficiency. (2022). https://pubmed.ncbi.nlm.nih.gov/35693943/ DOI: 10.3389/fcell.2022.903953
- tissue_or_cell_type
- HEK293 cells and secretory glycoproteins
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Manganese: enzyme cofactors, glycosylation, transport and nutrient interactions (2026-09-17) · lines 810–822
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Control and TMEM165-knockout HEK293 glycosylation assays · source_derived_draft · unverified_draft
### mn-gly-gal-o-linked-limited D-galactose partially improved the 24-hour O-glycan lectin readout but failed to rescue the three-day benzyl-GalNAc mass-spectrometry phenotype in TMEM165-knockout HEK cells. Condition category: machinery_impairment nutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect. plain_language: One O-glycan test improved partly while a different test remained abnormal; galactose did not normalize this pathway. organism: Homo sapiens tissue_or_cell_type: HEK293 cells and secretory glycoproteins experimental_model: Control and TMEM165-knockout HEK293 glycosylation assays limitations: Cell rescue is not evidence for a safe human dose. N-linked, mucin-type O-linked and proteoglycan GAG endpoints are distinct; restoring one is not proof of global correction. exposure: Figure 3 lectin staining: 2.5 micromolar MnCl2 and/or 1 millimolar galactose for 24 hours. Figure 4: same additions with 250 micromolar benzyl-GalNAc for three days. cross_nutrient: TMEM165 Golgi cation-homeostasis protein (affected_protein); Manganese(II) ion (effective_comparator) evidence_span: {"source_cache": "artifacts/manganese-glycosylation-sources/morelle2022.txt", "locator": "Results; O-linked lectin and mass-spectrometry endpoints", "file_sha256": "91d05677c6d7bb4224f71742888e39533e3f130d59e6b2ffcae3e2b2c058e9aa", "start_char": 19358, "end_char": 22255, "text_sha256": "e0b72a5427217107308f73e2304f47c761a25448096f516ca75c497eb8e389d5", "text_characters": 2897} [mn-gly-morelle2022] Differential Effects of D-Galactose Supplementation on Golgi Glycosylation Defects in TMEM165 Deficiency. (2022). https://pubmed.ncbi.nlm.nih.gov/35693943/ DOI: 10.3389/fcell.2022.903953
Complete structured claim and evidenceMnCl2 restored the measured O-linked glycosylation defects in TMEM165-knockout HEK cells.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- Manganese(II) ion (supplied_ion); TMEM165 Golgi cation-homeostasis protein (affected_protein)
- evidence_span
- {"source_cache": "artifacts/manganese-glycosylation-sources/morelle2022.txt", "locator": "Results; lectin staining and benzyl-GalNAc mass-spectrometry profiles", "file_sha256": "91d05677c6d7bb4224f71742888e39533e3f130d59e6b2ffcae3e2b2c058e9aa", "start_char": 24441, "end_char": 25329, "text_sha256": "40d6513f6782e8f624ca1d04f64d8153282e2beed8877766a60140128e1b438d", "text_characters": 888}
- experimental_model
- Control and TMEM165-knockout HEK293 glycosylation assays
- exposure
- Figure 3 lectin staining: 2.5 micromolar MnCl2 and/or 1 millimolar galactose for 24 hours. Figure 4: same additions with 250 micromolar benzyl-GalNAc for three days.
- limitations
- Cell rescue is not evidence for a safe human dose. N-linked, mucin-type O-linked and proteoglycan GAG endpoints are distinct; restoring one is not proof of global correction.
- nutrient_topic
- Manganese research collection; topical membership is not evidence of a direct dietary effect. · Manganese
- organism
- Homo sapiens
- plain_language
- Manganese also improved a different class of sugar chains.
- primary_references
- [mn-gly-morelle2022] Differential Effects of D-Galactose Supplementation on Golgi Glycosylation Defects in TMEM165 Deficiency. (2022). https://pubmed.ncbi.nlm.nih.gov/35693943/ DOI: 10.3389/fcell.2022.903953
- tissue_or_cell_type
- HEK293 cells and secretory glycoproteins
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Manganese: enzyme cofactors, glycosylation, transport and nutrient interactions (2026-09-17) · lines 768–780
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Control and TMEM165-knockout HEK293 glycosylation assays · source_derived_draft · unverified_draft
### mn-gly-mn-o-linked-rescue MnCl2 restored the measured O-linked glycosylation defects in TMEM165-knockout HEK cells. Condition category: machinery_impairment nutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect. plain_language: Manganese also improved a different class of sugar chains. organism: Homo sapiens tissue_or_cell_type: HEK293 cells and secretory glycoproteins experimental_model: Control and TMEM165-knockout HEK293 glycosylation assays limitations: Cell rescue is not evidence for a safe human dose. N-linked, mucin-type O-linked and proteoglycan GAG endpoints are distinct; restoring one is not proof of global correction. exposure: Figure 3 lectin staining: 2.5 micromolar MnCl2 and/or 1 millimolar galactose for 24 hours. Figure 4: same additions with 250 micromolar benzyl-GalNAc for three days. cross_nutrient: Manganese(II) ion (supplied_ion); TMEM165 Golgi cation-homeostasis protein (affected_protein) evidence_span: {"source_cache": "artifacts/manganese-glycosylation-sources/morelle2022.txt", "locator": "Results; lectin staining and benzyl-GalNAc mass-spectrometry profiles", "file_sha256": "91d05677c6d7bb4224f71742888e39533e3f130d59e6b2ffcae3e2b2c058e9aa", "start_char": 24441, "end_char": 25329, "text_sha256": "40d6513f6782e8f624ca1d04f64d8153282e2beed8877766a60140128e1b438d", "text_characters": 888} [mn-gly-morelle2022] Differential Effects of D-Galactose Supplementation on Golgi Glycosylation Defects in TMEM165 Deficiency. (2022). https://pubmed.ncbi.nlm.nih.gov/35693943/ DOI: 10.3389/fcell.2022.903953
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.