{"id":"08236684-828f-5c12-8229-ec80eb527d03","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-gly-gal-o-linked-limited","predicate":"did_not_fully_rescue","statement":"D-galactose partially improved the 24-hour O-glycan lectin readout but failed to rescue the three-day benzyl-GalNAc mass-spectrometry phenotype in TMEM165-knockout HEK cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"339e9717-54b0-5c3e-9108-4712b97a2af0","mechanism_event_label":"One O-glycan test improved partly while a different test remained abnormal; galactose did not normalize this pathway.","subject":{"id":"752497bc-783a-54f4-b7dc-72e48f2661fe","slug":"d-galactose","display_name":"D-Galactose","entity_type_key":"small_molecule"},"object":{"id":"75770cf2-398f-5a01-a14d-a461dff3be10","slug":"mucin-type-o-glycosylation","display_name":"Mucin-type O-linked glycosylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"339e9717-54b0-5c3e-9108-4712b97a2af0","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-gly-gal-o-linked-limited-event","event_type":"biochemical_relationship","label":"One O-glycan test improved partly while a different test remained abnormal; galactose did not normalize this pathway.","description":"D-galactose partially improved the 24-hour O-glycan lectin readout but failed to rescue the three-day benzyl-GalNAc mass-spectrometry phenotype in TMEM165-knockout HEK cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"567f9468-661b-59f8-a50d-408fe2133a87","slug":"tmem165","display_name":"TMEM165 Golgi cation-homeostasis protein","entity_type_key":"protein"},"role":"affected_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"effective_comparator","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"752497bc-783a-54f4-b7dc-72e48f2661fe","slug":"d-galactose","display_name":"D-Galactose","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"75770cf2-398f-5a01-a14d-a461dff3be10","slug":"mucin-type-o-glycosylation","display_name":"Mucin-type O-linked glycosylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"TMEM165 Golgi cation-homeostasis protein (affected_protein); Manganese(II) ion (effective_comparator)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/manganese-glycosylation-sources/morelle2022.txt\", \"locator\": \"Results; O-linked lectin and mass-spectrometry endpoints\", \"file_sha256\": \"91d05677c6d7bb4224f71742888e39533e3f130d59e6b2ffcae3e2b2c058e9aa\", \"start_char\": 19358, \"end_char\": 22255, \"text_sha256\": \"e0b72a5427217107308f73e2304f47c761a25448096f516ca75c497eb8e389d5\", \"text_characters\": 2897}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Control and TMEM165-knockout HEK293 glycosylation assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Figure 3 lectin staining: 2.5 micromolar MnCl2 and/or 1 millimolar galactose for 24 hours. 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N-linked, mucin-type O-linked and proteoglycan GAG endpoints are distinct; restoring one is not proof of global correction.\nexposure: Figure 3 lectin staining: 2.5 micromolar MnCl2 and/or 1 millimolar galactose for 24 hours. Figure 4: same additions with 250 micromolar benzyl-GalNAc for three days.\ncross_nutrient: TMEM165 Golgi cation-homeostasis protein (affected_protein); Manganese(II) ion (effective_comparator)\nevidence_span: {\"source_cache\": \"artifacts/manganese-glycosylation-sources/morelle2022.txt\", \"locator\": \"Results; O-linked lectin and mass-spectrometry endpoints\", \"file_sha256\": \"91d05677c6d7bb4224f71742888e39533e3f130d59e6b2ffcae3e2b2c058e9aa\", \"start_char\": 19358, \"end_char\": 22255, \"text_sha256\": \"e0b72a5427217107308f73e2304f47c761a25448096f516ca75c497eb8e389d5\", \"text_characters\": 2897}\n[mn-gly-morelle2022] Differential Effects of D-Galactose Supplementation on Golgi Glycosylation Defects in TMEM165 Deficiency. 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