Component
Mouse reduced folate carrier / Slc19a1
Mouse reduced folate carrier / Slc19a1; species and subcellular isoforms retained separately.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
RFC overexpression increased thiamine-monophosphate influx approximately fivefold in mouse R16 leukemia cells relative to the parent line.
Experimental context and source evidence
- cross_nutrient
- B1-folate: shared carrier for folate and TMP; free thiamine is a different substrate.
- experimental_model
- Mouse L1210/R16 leukemia transport experiments
- exposure
- Short radiolabeled TMP uptake assays
- limitations
- Extracellular TMP hydrolysis and transformed cells constrain extrapolation.
- nutrient_topic
- Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. · Folate (vitamin B9)
- organism
- Mus musculus
- plain_language
- A folate carrier also transported a phosphorylated B1 form.
- primary_references
- [zhao2002] Reduced folate carrier transports thiamine monophosphate: an alternative route for thiamine delivery into mammalian cells (2002). https://pubmed.ncbi.nlm.nih.gov/11997266/ DOI: 10.1152/ajpcell.00547.2001
- tissue_or_cell_type
- L1210/R16 leukemia cells
- transport_effect
- raises Overexpression increased thiamine-monophosphate influx about fivefold.
- transport_pool
- the expressing cell Overexpression increased thiamine-monophosphate influx about fivefold.
Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 180–191
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Mouse L1210/R16 leukemia transport experiments · source_derived_draft · unverified_draft
### folate-mouse-rfc-tmp-entry RFC overexpression increased thiamine-monophosphate influx approximately fivefold in mouse R16 leukemia cells relative to the parent line. Condition category: normal nutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: A folate carrier also transported a phosphorylated B1 form. organism: Mus musculus tissue_or_cell_type: L1210/R16 leukemia cells experimental_model: Mouse L1210/R16 leukemia transport experiments limitations: Extracellular TMP hydrolysis and transformed cells constrain extrapolation. exposure: Short radiolabeled TMP uptake assays cross_nutrient: B1-folate: shared carrier for folate and TMP; free thiamine is a different substrate. [zhao2002] Reduced folate carrier transports thiamine monophosphate: an alternative route for thiamine delivery into mammalian cells (2002). https://pubmed.ncbi.nlm.nih.gov/11997266/ DOI: 10.1152/ajpcell.00547.2001
Complete structured claim and evidence
Where it participates (unsigned role)
Methotrexate inhibited the RFC-mediated component of thiamine-monophosphate influx in mouse L1210 cells.
Experimental context and source evidence
- cross_nutrient
- B1-antifolate: competition at RFC under the tested drug exposure.
- experimental_model
- Mouse L1210/R16 leukemia transport experiments
- exposure
- 50 nM TMP with 100 micromolar methotrexate
- limitations
- Pharmacological cell experiment, not a clinical thiamine-deficiency outcome.
- nutrient_topic
- Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. · Folate (vitamin B9)
- organism
- Mus musculus
- plain_language
- An antifolate competed with phosphorylated B1 entry.
- primary_references
- [zhao2002] Reduced folate carrier transports thiamine monophosphate: an alternative route for thiamine delivery into mammalian cells (2002). https://pubmed.ncbi.nlm.nih.gov/11997266/ DOI: 10.1152/ajpcell.00547.2001
- tissue_or_cell_type
- L1210 leukemia cells
Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 193–204
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Mouse L1210/R16 leukemia transport experiments · source_derived_draft · unverified_draft
### folate-mtx-tmp-transport-competition Methotrexate inhibited the RFC-mediated component of thiamine-monophosphate influx in mouse L1210 cells. Condition category: normal nutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: An antifolate competed with phosphorylated B1 entry. organism: Mus musculus tissue_or_cell_type: L1210 leukemia cells experimental_model: Mouse L1210/R16 leukemia transport experiments limitations: Pharmacological cell experiment, not a clinical thiamine-deficiency outcome. exposure: 50 nM TMP with 100 micromolar methotrexate cross_nutrient: B1-antifolate: competition at RFC under the tested drug exposure. [zhao2002] Reduced folate carrier transports thiamine monophosphate: an alternative route for thiamine delivery into mammalian cells (2002). https://pubmed.ncbi.nlm.nih.gov/11997266/ DOI: 10.1152/ajpcell.00547.2001
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.