{"id":"72b57fe3-3974-57bc-a7e0-ba98abff1ab7","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-mouse-rfc-tmp-entry","predicate":"transports","statement":"RFC overexpression increased thiamine-monophosphate influx approximately fivefold in mouse R16 leukemia cells relative to the parent line.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b7263c07-1c73-5313-8870-4c6f15f34a70","mechanism_event_label":"A folate carrier also transported a phosphorylated B1 form.","subject":{"id":"91f4365d-4a71-564a-a40d-088e23f03914","slug":"mouse-slc19a1","display_name":"Mouse reduced folate carrier / Slc19a1","entity_type_key":"protein"},"object":{"id":"2d52919c-025a-5772-92b0-6229c0b74ba3","slug":"thiamine-monophosphate","display_name":"Thiamine monophosphate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b7263c07-1c73-5313-8870-4c6f15f34a70","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-mouse-rfc-tmp-entry-event","event_type":"biochemical_relationship","label":"A folate carrier also transported a phosphorylated B1 form.","description":"RFC overexpression increased thiamine-monophosphate influx approximately fivefold in mouse R16 leukemia cells relative to the parent line.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"91f4365d-4a71-564a-a40d-088e23f03914","slug":"mouse-slc19a1","display_name":"Mouse reduced folate carrier / Slc19a1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2d52919c-025a-5772-92b0-6229c0b74ba3","slug":"thiamine-monophosphate","display_name":"Thiamine monophosphate","entity_type_key":"small_molecule"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"parent nutrient","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"B1-folate: shared carrier for folate and TMP; free thiamine is a different substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse L1210/R16 leukemia transport experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Short radiolabeled TMP uptake assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Extracellular TMP hydrolysis and transformed cells constrain extrapolation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A folate carrier also transported a phosphorylated B1 form.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zhao2002] Reduced folate carrier transports thiamine monophosphate: an alternative route for thiamine delivery into mammalian cells (2002). https://pubmed.ncbi.nlm.nih.gov/11997266/ DOI: 10.1152/ajpcell.00547.2001","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"L1210/R16 leukemia cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Overexpression increased thiamine-monophosphate influx about fivefold.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Overexpression increased thiamine-monophosphate influx about fivefold.","entity":null}],"evidence":[{"id":"748733ca-ac87-5f9c-8e01-40ae5104d46a","evidence_kind":"source_excerpt","locator":"Lines 180-191","start_line":180,"end_line":191,"excerpt":"### folate-mouse-rfc-tmp-entry\nRFC overexpression increased thiamine-monophosphate influx approximately fivefold in mouse R16 leukemia cells relative to the parent line.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A folate carrier also transported a phosphorylated B1 form.\norganism: Mus musculus\ntissue_or_cell_type: L1210/R16 leukemia cells\nexperimental_model: Mouse L1210/R16 leukemia transport experiments\nlimitations: Extracellular TMP hydrolysis and transformed cells constrain extrapolation.\nexposure: Short radiolabeled TMP uptake assays\ncross_nutrient: B1-folate: shared carrier for folate and TMP; free thiamine is a different substrate.\n[zhao2002] Reduced folate carrier transports thiamine monophosphate: an alternative route for thiamine delivery into mammalian cells (2002). https://pubmed.ncbi.nlm.nih.gov/11997266/ DOI: 10.1152/ajpcell.00547.2001","model_system":"Mouse L1210/R16 leukemia transport experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zhao2002] Reduced folate carrier transports thiamine monophosphate: an alternative route for thiamine delivery into mammalian cells (2002). https://pubmed.ncbi.nlm.nih.gov/11997266/ DOI: 10.1152/ajpcell.00547.2001","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}