Component

Cardiomyocyte-targeted mouse Dlat shRNA knockdown

Study-specific entity. Experimental conditions and evidence limits remain on each linked claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

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How nutrients influence it

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Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

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What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. Cardiac Dlat knockdown reduced mitochondrial protein hyperacetylation in the two-hit HFpEF model.

    Cardiomyocyte-targeted mouse Dlat shRNA knockdown → Mouse cardiac mitochondrial protein lysine acetylation source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Cardiac tissue / mitochondria
    dose
    Two-hit model: 60% fat-calorie diet and L-NAME 0.5 g/L drinking water; vector dose not reported in reviewed text
    duration
    Two-hit protocol 15 weeks. Transgenic age/induction wording differs across passages; do not infer one exact interval
    endpoint
    mouse-mitochondrial-protein-acetylation
    evidence_location
    Figure 5C/D
    experimental_model
    Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated
    exposure
    mouse-dlat-knockdown
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects.
    organism
    Mus musculus
    plain_language
    Cardiac Dlat knockdown reduced mitochondrial protein hyperacetylation in the two-hit HFpEF model.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 40, "text_sha256": "243609ee6c00a8f0af6ff98eb44fa8397862deb054738dfa97ffabdbb7be4763", "xml_element_id": "Par18"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 42, "text_sha256": "6736653063633e10bc6238c895766205418e072440d0bb94d0415def1a9ec579", "xml_element_id": null}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    6 overall; 3 per individual protein panel
    tissue_or_cell_type
    Cardiac tissue and cardiomyocytes

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 424–439

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated · source_derived_draft · unverified_draft

    Cardiac Dlat knockdown reduced mitochondrial protein hyperacetylation in the two-hit HFpEF model. organism: Mus musculus tissue_or_cell_type: Cardiac tissue and cardiomyocytes experimental_model: Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated compartment: Cardiac tissue / mitochondria dose: Two-hit model: 60% fat-calorie diet and L-NAME 0.5 g/L drinking water; vector dose not reported in reviewed text duration: Two-hit protocol 15 weeks. Transgenic age/induction wording differs across passages; do not infer one exact interval primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Figure 5C/D endpoint: mouse-mitochondrial-protein-acetylation exposure: mouse-dlat-knockdown limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 40, "text_sha256": "243609ee6c00a8f0af6ff98eb44fa8397862deb054738dfa97ffabdbb7be4763", "xml_element_id": "Par18"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 42, "text_sha256": "6736653063633e10bc6238c895766205418e072440d0bb94d0415def1a9ec579", "xml_element_id": null}] plain_language: Cardiac Dlat knockdown reduced mitochondrial protein hyperacetylation in the two-hit HFpEF model. sample_size: 6 overall; 3 per individual protein panel
    Complete structured claim and evidence

  2. Partial Dlat knockdown did not substantially change the reported FAO capacity of control mouse hearts.

    Cardiomyocyte-targeted mouse Dlat shRNA knockdown → Mouse cardiac fatty-acid oxidation capacity source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Cardiac tissue / mitochondria
    dose
    Partial AAV9-mediated Dlat knockdown; vector dose unresolved
    duration
    Endpoint interval not resolved for this control arm
    endpoint
    mouse-cardiac-fao
    evidence_location
    Control+AAV9-sh-Dlat versus control+AAV9-NC; Figure 5
    experimental_model
    Non-HFpEF control hearts with partial AAV9-mediated Dlat knockdown
    exposure
    mouse-dlat-knockdown
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. A context-specific null finding with partial knockdown; not proof that total Dlat loss is harmless.
    organism
    Mus musculus
    plain_language
    Partial Dlat knockdown did not substantially change the reported FAO capacity of control mouse hearts.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 40, "text_sha256": "243609ee6c00a8f0af6ff98eb44fa8397862deb054738dfa97ffabdbb7be4763", "xml_element_id": "Par18"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 42, "text_sha256": "6736653063633e10bc6238c895766205418e072440d0bb94d0415def1a9ec579", "xml_element_id": null}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    tissue_or_cell_type
    Cardiac tissue and cardiomyocytes

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 442–456

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Non-HFpEF control hearts with partial AAV9-mediated Dlat knockdown · source_derived_draft · unverified_draft

    Partial Dlat knockdown did not substantially change the reported FAO capacity of control mouse hearts. organism: Mus musculus tissue_or_cell_type: Cardiac tissue and cardiomyocytes experimental_model: Non-HFpEF control hearts with partial AAV9-mediated Dlat knockdown compartment: Cardiac tissue / mitochondria dose: Partial AAV9-mediated Dlat knockdown; vector dose unresolved duration: Endpoint interval not resolved for this control arm primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Control+AAV9-sh-Dlat versus control+AAV9-NC; Figure 5 endpoint: mouse-cardiac-fao exposure: mouse-dlat-knockdown limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. A context-specific null finding with partial knockdown; not proof that total Dlat loss is harmless. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 40, "text_sha256": "243609ee6c00a8f0af6ff98eb44fa8397862deb054738dfa97ffabdbb7be4763", "xml_element_id": "Par18"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 42, "text_sha256": "6736653063633e10bc6238c895766205418e072440d0bb94d0415def1a9ec579", "xml_element_id": null}] plain_language: Partial Dlat knockdown did not substantially change the reported FAO capacity of control mouse hearts.
    Complete structured claim and evidence

  3. Cardiac Dlat knockdown improved substrate-supported respiration in the two-hit HFpEF model.

    Cardiomyocyte-targeted mouse Dlat shRNA knockdown → Mouse cardiac fatty-acid oxidation capacity source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Cardiac tissue / mitochondria
    dose
    Two-hit model: 60% fat-calorie diet and L-NAME 0.5 g/L drinking water; vector dose not reported in reviewed text
    duration
    Two-hit protocol 15 weeks. Transgenic age/induction wording differs across passages; do not infer one exact interval
    endpoint
    mouse-cardiac-fao
    evidence_location
    AAV9-cTnT-Dlat shRNA and Oroboros respirometry; Figure 5E–G
    experimental_model
    Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated
    exposure
    mouse-dlat-knockdown
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Partial knockdown, not complete deletion; not evidence that systemic DLAT inhibition is safe.
    organism
    Mus musculus
    plain_language
    Cardiac Dlat knockdown improved substrate-supported respiration in the two-hit HFpEF model.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 40, "text_sha256": "243609ee6c00a8f0af6ff98eb44fa8397862deb054738dfa97ffabdbb7be4763", "xml_element_id": "Par18"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 42, "text_sha256": "6736653063633e10bc6238c895766205418e072440d0bb94d0415def1a9ec579", "xml_element_id": null}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    4 biological replicates
    tissue_or_cell_type
    Cardiac tissue and cardiomyocytes

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 406–421

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated · source_derived_draft · unverified_draft

    Cardiac Dlat knockdown improved substrate-supported respiration in the two-hit HFpEF model. organism: Mus musculus tissue_or_cell_type: Cardiac tissue and cardiomyocytes experimental_model: Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated compartment: Cardiac tissue / mitochondria dose: Two-hit model: 60% fat-calorie diet and L-NAME 0.5 g/L drinking water; vector dose not reported in reviewed text duration: Two-hit protocol 15 weeks. Transgenic age/induction wording differs across passages; do not infer one exact interval primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: AAV9-cTnT-Dlat shRNA and Oroboros respirometry; Figure 5E–G endpoint: mouse-cardiac-fao exposure: mouse-dlat-knockdown limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Partial knockdown, not complete deletion; not evidence that systemic DLAT inhibition is safe. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 40, "text_sha256": "243609ee6c00a8f0af6ff98eb44fa8397862deb054738dfa97ffabdbb7be4763", "xml_element_id": "Par18"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 42, "text_sha256": "6736653063633e10bc6238c895766205418e072440d0bb94d0415def1a9ec579", "xml_element_id": null}] plain_language: Cardiac Dlat knockdown improved substrate-supported respiration in the two-hit HFpEF model. sample_size: 4 biological replicates
    Complete structured claim and evidence

  4. Dlat knockdown improved the reported diastolic phenotype in the two-hit HFpEF model.

    Cardiomyocyte-targeted mouse Dlat shRNA knockdown → Mouse cardiac diastolic impairment source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Cardiac tissue / mitochondria
    dose
    Two-hit model: 60% fat-calorie diet and L-NAME 0.5 g/L drinking water; vector dose not reported in reviewed text
    duration
    Two-hit protocol 15 weeks. Transgenic age/induction wording differs across passages; do not infer one exact interval
    endpoint
    mouse-cardiac-diastolic-impairment
    evidence_location
    Figure 5K–M; source-data workbook figure5 L/M
    experimental_model
    Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated
    exposure
    mouse-dlat-knockdown
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. The prose says E/e-prime increased with improvement, whereas the plotted/source values decrease versus HFpEF control. Record the functional interpretation with this source inconsistency, not the mistaken metric direction.
    organism
    Mus musculus
    plain_language
    Dlat knockdown improved the reported diastolic phenotype in the two-hit HFpEF model.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 40, "text_sha256": "243609ee6c00a8f0af6ff98eb44fa8397862deb054738dfa97ffabdbb7be4763", "xml_element_id": "Par18"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 42, "text_sha256": "6736653063633e10bc6238c895766205418e072440d0bb94d0415def1a9ec579", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/41467_2026_70703_MOESM4_ESM.xlsx", "location": "figure5; E/A and E/e-prime rows 41-50", "sha256": "7fc506fec970a4b7e9b2f370695d82b36020de2e77a0a26112066688d52606f5"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    8 per group
    tissue_or_cell_type
    Cardiac tissue and cardiomyocytes

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 459–474

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated · source_derived_draft · unverified_draft

    Dlat knockdown improved the reported diastolic phenotype in the two-hit HFpEF model. organism: Mus musculus tissue_or_cell_type: Cardiac tissue and cardiomyocytes experimental_model: Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated compartment: Cardiac tissue / mitochondria dose: Two-hit model: 60% fat-calorie diet and L-NAME 0.5 g/L drinking water; vector dose not reported in reviewed text duration: Two-hit protocol 15 weeks. Transgenic age/induction wording differs across passages; do not infer one exact interval primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Figure 5K–M; source-data workbook figure5 L/M endpoint: mouse-cardiac-diastolic-impairment exposure: mouse-dlat-knockdown limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. The prose says E/e-prime increased with improvement, whereas the plotted/source values decrease versus HFpEF control. Record the functional interpretation with this source inconsistency, not the mistaken metric direction. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 40, "text_sha256": "243609ee6c00a8f0af6ff98eb44fa8397862deb054738dfa97ffabdbb7be4763", "xml_element_id": "Par18"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 42, "text_sha256": "6736653063633e10bc6238c895766205418e072440d0bb94d0415def1a9ec579", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/41467_2026_70703_MOESM4_ESM.xlsx", "location": "figure5; E/A and E/e-prime rows 41-50", "sha256": "7fc506fec970a4b7e9b2f370695d82b36020de2e77a0a26112066688d52606f5"}] plain_language: Dlat knockdown improved the reported diastolic phenotype in the two-hit HFpEF model. sample_size: 8 per group
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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