{"id":"0d5d31cb-b48e-5311-a111-b7be31761b21","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:mouse-dlat-kd-control","predicate":"has_no_detected_effect","statement":"Partial Dlat knockdown did not substantially change the reported FAO capacity of control mouse hearts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"cdbcf85c-11d8-5783-b43e-231e2c1ebbcd","mechanism_event_label":"mouse dlat kd control","subject":{"id":"d3f48501-673f-562e-b48b-9a10f2b25237","slug":"mouse-dlat-knockdown","display_name":"Cardiomyocyte-targeted mouse Dlat shRNA knockdown","entity_type_key":"protein_state"},"object":{"id":"4277e9b5-6f0b-55b4-89b1-3201b6182f04","slug":"mouse-cardiac-fao","display_name":"Mouse cardiac fatty-acid oxidation capacity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cdbcf85c-11d8-5783-b43e-231e2c1ebbcd","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:mouse-dlat-kd-control","event_type":"has_no_detected_effect","label":"mouse dlat kd control","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d3f48501-673f-562e-b48b-9a10f2b25237","slug":"mouse-dlat-knockdown","display_name":"Cardiomyocyte-targeted mouse Dlat shRNA knockdown","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4277e9b5-6f0b-55b4-89b1-3201b6182f04","slug":"mouse-cardiac-fao","display_name":"Mouse cardiac fatty-acid oxidation capacity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Cardiac tissue / mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Partial AAV9-mediated Dlat knockdown; vector dose unresolved","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Endpoint interval not resolved for this control arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"mouse-cardiac-fao","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Control+AAV9-sh-Dlat versus control+AAV9-NC; Figure 5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Non-HFpEF control hearts with partial AAV9-mediated Dlat knockdown","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"mouse-dlat-knockdown","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. A context-specific null finding with partial knockdown; not proof that total Dlat loss is harmless.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Partial Dlat knockdown did not substantially change the reported FAO capacity of control mouse hearts.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 40, \"text_sha256\": \"243609ee6c00a8f0af6ff98eb44fa8397862deb054738dfa97ffabdbb7be4763\", \"xml_element_id\": \"Par18\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 42, \"text_sha256\": \"6736653063633e10bc6238c895766205418e072440d0bb94d0415def1a9ec579\", \"xml_element_id\": null}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cardiac tissue and cardiomyocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"02a9484d-9af9-5fc7-b145-c30a29413cba","evidence_kind":"source_excerpt","locator":"Lines 442-456","start_line":442,"end_line":456,"excerpt":"Partial Dlat knockdown did not substantially change the reported FAO capacity of control mouse hearts.\norganism: Mus musculus\ntissue_or_cell_type: Cardiac tissue and cardiomyocytes\nexperimental_model: Non-HFpEF control hearts with partial AAV9-mediated Dlat knockdown\ncompartment: Cardiac tissue / mitochondria\ndose: Partial AAV9-mediated Dlat knockdown; vector dose unresolved\nduration: Endpoint interval not resolved for this control arm\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Control+AAV9-sh-Dlat versus control+AAV9-NC; Figure 5\nendpoint: mouse-cardiac-fao\nexposure: mouse-dlat-knockdown\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. A context-specific null finding with partial knockdown; not proof that total Dlat loss is harmless.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 40, \"text_sha256\": \"243609ee6c00a8f0af6ff98eb44fa8397862deb054738dfa97ffabdbb7be4763\", \"xml_element_id\": \"Par18\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 42, \"text_sha256\": \"6736653063633e10bc6238c895766205418e072440d0bb94d0415def1a9ec579\", \"xml_element_id\": null}]\nplain_language: Partial Dlat knockdown did not substantially change the reported FAO capacity of control mouse hearts.","model_system":"Non-HFpEF control hearts with partial AAV9-mediated Dlat knockdown","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}