Component
Mammalian GSK3 preparation in lithium competition assays
Context-specific entity; species, compartment and exposure are stated on each claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
ATP concentrations exceeding magnesium progressively inhibited GSK3 in the assay through free-magnesium chelation.
Experimental context and source evidence
- evidence_access
- Primary abstract
- experimental_model
- Biochemical concentration titrations.
- limitations
- This is assay speciation, not evidence that lithium exhausts ATP or that extra magnesium improves clinical outcomes.
- nutrient_topic
- Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Lithium
- plain_language
- ATP and free magnesium must be considered together.
- primary_references
- Lithium inhibits glycogen synthase kinase-3 by competition for magnesium. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11162580/ · DOI 10.1006/bbrc.2000.4169
Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19) · lines 24–30
AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Biochemical concentration titrations. · source_derived_draft · unverified_draft
## lithium-gsk3-atp-magnesium ATP and free magnesium must be considered together. ATP concentrations exceeding magnesium progressively inhibited GSK3 in the assay through free-magnesium chelation. Model: Biochemical concentration titrations. Limitations: This is assay speciation, not evidence that lithium exhausts ATP or that extra magnesium improves clinical outcomes. Evidence access: Primary abstract Lithium inhibits glycogen synthase kinase-3 by competition for magnesium. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11162580/ · DOI 10.1006/bbrc.2000.4169
Complete structured claim and evidenceLithium competitively inhibited mammalian GSK3 with respect to magnesium, but not ATP or substrate.
Experimental context and source evidence
- evidence_access
- Primary abstract
- experimental_model
- Purified GSK3 kinetic experiments; mammalian and Dictyostelium isoforms.
- limitations
- The abstract does not resolve every mammalian isoform; no systemic magnesium depletion was measured.
- nutrient_topic
- Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Lithium
- plain_language
- Magnesium availability changes inhibition of a signaling enzyme.
- primary_references
- Lithium inhibits glycogen synthase kinase-3 by competition for magnesium. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11162580/ · DOI 10.1006/bbrc.2000.4169
Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19) · lines 16–22
AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Purified GSK3 kinetic experiments; mammalian and Dictyostelium isoforms. · source_derived_draft · unverified_draft
## lithium-gsk3-magnesium Magnesium availability changes inhibition of a signaling enzyme. Lithium competitively inhibited mammalian GSK3 with respect to magnesium, but not ATP or substrate. Model: Purified GSK3 kinetic experiments; mammalian and Dictyostelium isoforms. Limitations: The abstract does not resolve every mammalian isoform; no systemic magnesium depletion was measured. Evidence access: Primary abstract Lithium inhibits glycogen synthase kinase-3 by competition for magnesium. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11162580/ · DOI 10.1006/bbrc.2000.4169
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.