{"id":"90d78ce5-bc2d-5820-af48-ffded5cde8ca","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-gsk3-atp-magnesium","predicate":"limits_free_magnesium_for","statement":"ATP concentrations exceeding magnesium progressively inhibited GSK3 in the assay through free-magnesium chelation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8f38c310-f85f-5357-9339-9c1db3051bc5","mechanism_event_label":"ATP and free magnesium must be considered together.","subject":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"object":{"id":"5bb47c43-4dad-5829-9bf2-586ea1d21007","slug":"mammalian-gsk3-biochemical-preparation","display_name":"Mammalian GSK3 preparation in lithium competition assays","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"8f38c310-f85f-5357-9339-9c1db3051bc5","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-gsk3-atp-magnesium-event","event_type":"observed_relationship","label":"ATP and free magnesium must be considered together.","description":"ATP concentrations exceeding magnesium progressively inhibited GSK3 in the assay through free-magnesium chelation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5bb47c43-4dad-5829-9bf2-586ea1d21007","slug":"mammalian-gsk3-biochemical-preparation","display_name":"Mammalian GSK3 preparation in lithium competition assays","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical concentration titrations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is assay speciation, not evidence that lithium exhausts ATP or that extra magnesium improves clinical outcomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"ATP and free magnesium must be considered together.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lithium inhibits glycogen synthase kinase-3 by competition for magnesium. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11162580/ · DOI 10.1006/bbrc.2000.4169","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bfb95627-1385-5743-a4fa-c0acd0fce5f3","evidence_kind":"source_excerpt","locator":"Lines 24-30","start_line":24,"end_line":30,"excerpt":"## lithium-gsk3-atp-magnesium\nATP and free magnesium must be considered together.\nATP concentrations exceeding magnesium progressively inhibited GSK3 in the assay through free-magnesium chelation.\nModel: Biochemical concentration titrations.\nLimitations: This is assay speciation, not evidence that lithium exhausts ATP or that extra magnesium improves clinical outcomes.\nEvidence access: Primary abstract\nLithium inhibits glycogen synthase kinase-3 by competition for magnesium. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11162580/ · DOI 10.1006/bbrc.2000.4169","model_system":"Biochemical concentration titrations.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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