{"id":"1309ed6a-226c-51a5-9f3f-5fdf8826e9b4","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-gsk3-magnesium","predicate":"competes_with_magnesium_at","statement":"Lithium competitively inhibited mammalian GSK3 with respect to magnesium, but not ATP or substrate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0fbc1fff-c4c5-5a31-832d-df6ff8a5acb2","mechanism_event_label":"Magnesium availability changes inhibition of a signaling enzyme.","subject":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"object":{"id":"5bb47c43-4dad-5829-9bf2-586ea1d21007","slug":"mammalian-gsk3-biochemical-preparation","display_name":"Mammalian GSK3 preparation in lithium competition assays","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"0fbc1fff-c4c5-5a31-832d-df6ff8a5acb2","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-gsk3-magnesium-event","event_type":"observed_relationship","label":"Magnesium availability changes inhibition of a signaling enzyme.","description":"Lithium competitively inhibited mammalian GSK3 with respect to magnesium, but not ATP or substrate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5bb47c43-4dad-5829-9bf2-586ea1d21007","slug":"mammalian-gsk3-biochemical-preparation","display_name":"Mammalian GSK3 preparation in lithium competition assays","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified GSK3 kinetic experiments; mammalian and Dictyostelium isoforms.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The abstract does not resolve every mammalian isoform; no systemic magnesium depletion was measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Magnesium availability changes inhibition of a signaling enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lithium inhibits glycogen synthase kinase-3 by competition for magnesium. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11162580/ · DOI 10.1006/bbrc.2000.4169","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ee94c0ec-9d95-504d-9088-1acae253f198","evidence_kind":"source_excerpt","locator":"Lines 16-22","start_line":16,"end_line":22,"excerpt":"## lithium-gsk3-magnesium\nMagnesium availability changes inhibition of a signaling enzyme.\nLithium competitively inhibited mammalian GSK3 with respect to magnesium, but not ATP or substrate.\nModel: Purified GSK3 kinetic experiments; mammalian and Dictyostelium isoforms.\nLimitations: The abstract does not resolve every mammalian isoform; no systemic magnesium depletion was measured.\nEvidence access: Primary abstract\nLithium inhibits glycogen synthase kinase-3 by competition for magnesium. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11162580/ · DOI 10.1006/bbrc.2000.4169","model_system":"Purified GSK3 kinetic experiments; mammalian and Dictyostelium isoforms.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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