Component

Leukocyte DNA methyl-acceptance assay

Independently recorded substance, clinical endpoint or assay readout. Claims retain study-specific population and measurement context.

2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. In vitro incorporation of labeled SAM methyl groups into extracted leukocyte DNA increased after depletion (P=0.0025), consistent with lower pre-existing methylation.

    Folate (vitamin B9) → Leukocyte DNA methyl-acceptance assay source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    nutrient_deficiency Imported condition classification; unverified.
    experimental_model
    Thirty-three women aged 60–85 in depletion, thirty in four repletion groups.
    exposure
    Seven weeks 118 micrograms/day folate, followed by seven weeks 200 or 415 micrograms/day with different food/supplement combinations.
    limitations
    The assay interpretation is distinct from measuring intracellular SAM or methylation at specific genes.
    nutrient_topic
    Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. · Folate (vitamin B9)
    organism
    Homo sapiens
    plain_language
    The assay found more available sites for adding methyl groups to the extracted DNA.
    primary_references
    [fol-rampersaud2000] Genomic DNA methylation decreases in response to moderate folate depletion in elderly women (2000). https://pubmed.ncbi.nlm.nih.gov/11010943/ DOI: 10.1093/ajcn/72.4.998
    tissue_or_cell_type
    Human leukocyte DNA, ex-vivo assay
    trigger_kind
    nutrient_deficiency Imported condition classification; unverified.

    Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1445–1455

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Thirty-three women aged 60–85 in depletion, thirty in four repletion groups. · source_derived_draft · unverified_draft

    ### fol-elderly-dna-methyl-acceptance In vitro incorporation of labeled SAM methyl groups into extracted leukocyte DNA increased after depletion (P=0.0025), consistent with lower pre-existing methylation. Condition category: nutrient_deficiency nutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: The assay found more available sites for adding methyl groups to the extracted DNA. organism: Homo sapiens tissue_or_cell_type: Human leukocyte DNA, ex-vivo assay experimental_model: Thirty-three women aged 60–85 in depletion, thirty in four repletion groups. limitations: The assay interpretation is distinct from measuring intracellular SAM or methylation at specific genes. exposure: Seven weeks 118 micrograms/day folate, followed by seven weeks 200 or 415 micrograms/day with different food/supplement combinations. [fol-rampersaud2000] Genomic DNA methylation decreases in response to moderate folate depletion in elderly women (2000). https://pubmed.ncbi.nlm.nih.gov/11010943/ DOI: 10.1093/ajcn/72.4.998
    Complete structured claim and evidence

  2. No repletion group showed a significant change from postdepletion methyl-acceptance values over seven weeks.

    Folate (vitamin B9) → Leukocyte DNA methyl-acceptance assay source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    nutrient_deficiency Imported condition classification; unverified.
    experimental_model
    Thirty-three women aged 60–85 in depletion, thirty in four repletion groups.
    exposure
    Seven weeks 118 micrograms/day folate, followed by seven weeks 200 or 415 micrograms/day with different food/supplement combinations.
    limitations
    Different age, intake and assay design from the eight-woman study; no claim of an unexplained scientific contradiction.
    nutrient_topic
    Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. · Folate (vitamin B9)
    organism
    Homo sapiens
    plain_language
    Returning folate did not quickly normalize this particular readout.
    primary_references
    [fol-rampersaud2000] Genomic DNA methylation decreases in response to moderate folate depletion in elderly women (2000). https://pubmed.ncbi.nlm.nih.gov/11010943/ DOI: 10.1093/ajcn/72.4.998
    tissue_or_cell_type
    Human leukocyte DNA, ex-vivo assay
    trigger_kind
    nutrient_deficiency Imported condition classification; unverified.

    Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1457–1467

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Thirty-three women aged 60–85 in depletion, thirty in four repletion groups. · source_derived_draft · unverified_draft

    ### fol-elderly-methylation-repletion-null No repletion group showed a significant change from postdepletion methyl-acceptance values over seven weeks. Condition category: nutrient_deficiency nutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: Returning folate did not quickly normalize this particular readout. organism: Homo sapiens tissue_or_cell_type: Human leukocyte DNA, ex-vivo assay experimental_model: Thirty-three women aged 60–85 in depletion, thirty in four repletion groups. limitations: Different age, intake and assay design from the eight-woman study; no claim of an unexplained scientific contradiction. exposure: Seven weeks 118 micrograms/day folate, followed by seven weeks 200 or 415 micrograms/day with different food/supplement combinations. [fol-rampersaud2000] Genomic DNA methylation decreases in response to moderate folate depletion in elderly women (2000). https://pubmed.ncbi.nlm.nih.gov/11010943/ DOI: 10.1093/ajcn/72.4.998
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards