{"id":"53cc913a-bd15-5e40-abb7-d5f21964dbd7","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-elderly-dna-methyl-acceptance","predicate":"depletion_increased_ex_vivo_methyl_acceptance","statement":"In vitro incorporation of labeled SAM methyl groups into extracted leukocyte DNA increased after depletion (P=0.0025), consistent with lower pre-existing methylation.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"c29c4fa4-0cfe-53e8-a507-284b942d0ff1","mechanism_event_label":"The assay found more available sites for adding methyl groups to the extracted DNA.","subject":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"object":{"id":"9e4efae7-15b8-5735-a045-18c0e733e2dc","slug":"leukocyte-dna-methyl-acceptance","display_name":"Leukocyte DNA methyl-acceptance assay","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c29c4fa4-0cfe-53e8-a507-284b942d0ff1","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-elderly-dna-methyl-acceptance-event","event_type":"observed_intervention","label":"The assay found more available sites for adding methyl groups to the extracted DNA.","description":"In vitro incorporation of labeled SAM methyl groups into extracted leukocyte DNA increased after depletion (P=0.0025), consistent with lower pre-existing methylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"assay_methyl_donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9e4efae7-15b8-5735-a045-18c0e733e2dc","slug":"leukocyte-dna-methyl-acceptance","display_name":"Leukocyte DNA methyl-acceptance assay","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; 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Study references: [fol-rampersaud2000] Genomic DNA methylation decreases in response to moderate folate depletion in elderly women (2000). https://pubmed.ncbi.nlm.nih.gov/11010943/ DOI: 10.1093/ajcn/72.4.998","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}