Component

Erythrocyte transketolase activation coefficient

Erythrocyte transketolase activation coefficient

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Infant ETK activation coefficient differed significantly only between the highest maternal-dose group and placebo, whereas infant ThDP differed for all active groups.

    Thiamine (vitamin B1) → Erythrocyte transketolase activation coefficient source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    biomarker_context Imported condition classification; unverified.
    experimental_model
    Same infant biomarker comparison.
    exposure
    Maternal 0, 1.2, 2.4 or 10 mg/day thiamine from weeks 2-24 postpartum; study exposures only.
    limitations
    Assay response is not a contradiction: endpoints differ. The study did not establish a universal clinical threshold.
    nutrient_topic
    Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
    organism
    Homo sapiens
    plain_language
    The concentration marker and functional assay did not identify identical dose responses.
    primary_references
    [b1-gallant2021] Low-dose thiamine supplementation of lactating Cambodian mothers improves human milk thiamine concentrations: a randomized controlled trial (2021). https://pubmed.ncbi.nlm.nih.gov/33829271/ DOI: 10.1093/ajcn/nqab052
    tissue_or_cell_type
    Infant blood
    trigger_kind
    biomarker_context Imported condition classification; unverified.

    Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1831–1841

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Same infant biomarker comparison. · source_derived_draft · unverified_draft

    ### b1-milk-trial-marker-difference Infant ETK activation coefficient differed significantly only between the highest maternal-dose group and placebo, whereas infant ThDP differed for all active groups. Condition category: biomarker_context nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: The concentration marker and functional assay did not identify identical dose responses. organism: Homo sapiens tissue_or_cell_type: Infant blood experimental_model: Same infant biomarker comparison. limitations: Assay response is not a contradiction: endpoints differ. The study did not establish a universal clinical threshold. exposure: Maternal 0, 1.2, 2.4 or 10 mg/day thiamine from weeks 2-24 postpartum; study exposures only. [b1-gallant2021] Low-dose thiamine supplementation of lactating Cambodian mothers improves human milk thiamine concentrations: a randomized controlled trial (2021). https://pubmed.ncbi.nlm.nih.gov/33829271/ DOI: 10.1093/ajcn/nqab052
    Complete structured claim and evidence

  2. Some at-risk patients had low erythrocyte ThDP with a normal activation test, and one had an abnormal activation test with normal ThDP.

    Erythrocyte thiamine diphosphate concentration → Erythrocyte transketolase activation coefficient source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    biomarker_context Imported condition classification; unverified.
    evidence_location
    Abstract Results; classification comparison
    evidence_span
    normal by the activation test but had low TDP
    experimental_model
    Human method-comparison study: HPLC erythrocyte/whole-blood ThDP, healthy reference cohorts and 63 patients considered at risk of deficiency compared with a transketolase activation test.
    exposure
    Laboratory assessment of thiamine status using erythrocytes or whole blood
    limitations
    Neither assay was independently validated against brain ThDP; no pooled sensitivity or specificity is inferred. The abstract contains inconsistent category totals, so no concordance percentage is calculated.
    nutrient_topic
    Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
    organism
    Homo sapiens
    plain_language
    The direct and functional tests sometimes classified the same patient differently.
    primary_references
    [talwar-2000-blood-thdp] Vitamin B(1) status assessed by direct measurement of thiamin pyrophosphate in erythrocytes or whole blood by HPLC: comparison with erythrocyte transketolase activation assay (2000). https://pubmed.ncbi.nlm.nih.gov/10794754/ DOI: 10.1093/clinchem/46.5.704
    tissue_or_cell_type
    Blood
    trigger_kind
    biomarker_context Imported condition classification; unverified.

    Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1423–1435

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human method-comparison study: HPLC erythrocyte/whole-blood ThDP, healthy reference cohorts and 63 patients considered at risk of deficiency compared with a transketolase activation test. · source_derived_draft · unverified_draft

    ### thiamine-def-direct-functional-assay-discordance Some at-risk patients had low erythrocyte ThDP with a normal activation test, and one had an abnormal activation test with normal ThDP. Condition category: biomarker_context nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: The direct and functional tests sometimes classified the same patient differently. organism: Homo sapiens tissue_or_cell_type: Blood experimental_model: Human method-comparison study: HPLC erythrocyte/whole-blood ThDP, healthy reference cohorts and 63 patients considered at risk of deficiency compared with a transketolase activation test. limitations: Neither assay was independently validated against brain ThDP; no pooled sensitivity or specificity is inferred. The abstract contains inconsistent category totals, so no concordance percentage is calculated. evidence_location: Abstract Results; classification comparison evidence_span: normal by the activation test but had low TDP exposure: Laboratory assessment of thiamine status using erythrocytes or whole blood [talwar-2000-blood-thdp] Vitamin B(1) status assessed by direct measurement of thiamin pyrophosphate in erythrocytes or whole blood by HPLC: comparison with erythrocyte transketolase activation assay (2000). https://pubmed.ncbi.nlm.nih.gov/10794754/ DOI: 10.1093/clinchem/46.5.704
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. The functional erythrocyte test compared transketolase activity before and after adding exogenous thiamine diphosphate.

    Thiamine diphosphate → Blood transketolase activity source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    biomarker_context Imported condition classification; unverified.
    evidence_location
    Abstract
    evidence_span
    before and after activation with exogenous thiamin diphosphate
    experimental_model
    Human erythrocytes incubated with thiamine, plus alcoholic, elderly and student comparison groups; HPLC ThDP versus basal and ThDP-stimulated transketolase assays.
    exposure
    Laboratory assessment of thiamine status using erythrocytes or whole blood
    limitations
    Assay response is influenced by the enzyme preparation and assay conditions; a small response is not a measurement of every tissue.
    nutrient_topic
    Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
    organism
    Homo sapiens
    plain_language
    This asks how much extra cofactor can activate the sampled enzyme, whereas HPLC counts the cofactor itself.
    primary_references
    [baines-1988-erythrocyte-assays] The Evaluation of Erythrocyte Thiamin Diphosphate as an Indicator of Thiamin Status in Man, and its Comparison with Erythrocyte Transketolase Activity Measurements (1988). https://journals.sagepub.com/doi/10.1177/000456328802500617 DOI: 10.1177/000456328802500617
    tissue_or_cell_type
    Erythrocyte hemolysates
    trigger_kind
    biomarker_context Imported condition classification; unverified.

    Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1381–1393

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human erythrocytes incubated with thiamine, plus alcoholic, elderly and student comparison groups; HPLC ThDP versus basal and ThDP-stimulated transketolase assays. · source_derived_draft · unverified_draft

    ### thiamine-def-exogenous-thdp-functional-assay The functional erythrocyte test compared transketolase activity before and after adding exogenous thiamine diphosphate. Condition category: biomarker_context nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: This asks how much extra cofactor can activate the sampled enzyme, whereas HPLC counts the cofactor itself. organism: Homo sapiens tissue_or_cell_type: Erythrocyte hemolysates experimental_model: Human erythrocytes incubated with thiamine, plus alcoholic, elderly and student comparison groups; HPLC ThDP versus basal and ThDP-stimulated transketolase assays. limitations: Assay response is influenced by the enzyme preparation and assay conditions; a small response is not a measurement of every tissue. evidence_location: Abstract evidence_span: before and after activation with exogenous thiamin diphosphate exposure: Laboratory assessment of thiamine status using erythrocytes or whole blood [baines-1988-erythrocyte-assays] The Evaluation of Erythrocyte Thiamin Diphosphate as an Indicator of Thiamin Status in Man, and its Comparison with Erythrocyte Transketolase Activity Measurements (1988). https://journals.sagepub.com/doi/10.1177/000456328802500617 DOI: 10.1177/000456328802500617
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards