{"id":"d327eae5-2f2d-51cd-8238-50dceeed672d","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-milk-trial-marker-difference","predicate":"maternal_supplementation_changes","statement":"Infant ETK activation coefficient differed significantly only between the highest maternal-dose group and placebo, whereas infant ThDP differed for all active groups.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2aa50efa-c08b-5b8e-a1a9-9fb7bbf1ab60","mechanism_event_label":"The concentration marker and functional assay did not identify identical dose responses.","subject":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"object":{"id":"94381007-9bd0-51cc-895d-988aa3eed6c6","slug":"erythrocyte-transketolase-activation-coefficient","display_name":"Erythrocyte transketolase activation coefficient","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2aa50efa-c08b-5b8e-a1a9-9fb7bbf1ab60","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-milk-trial-marker-difference-event","event_type":"observed_intervention","label":"The concentration marker and functional assay did not identify identical dose responses.","description":"Infant ETK activation coefficient differed significantly only between the highest maternal-dose group and placebo, whereas infant ThDP differed for all active groups.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"97206826-3d6b-5466-ab3c-407351eb0c93","slug":"whole-blood-thdp-concentration","display_name":"Whole-blood thiamine diphosphate concentration","entity_type_key":"cellular_process"},"role":"different_endpoint","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c95c645c-ae67-5911-871e-4f796a17faec","slug":"tkt","display_name":"Human transketolase","entity_type_key":"protein"},"role":"functional_assay_target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"94381007-9bd0-51cc-895d-988aa3eed6c6","slug":"erythrocyte-transketolase-activation-coefficient","display_name":"Erythrocyte transketolase activation coefficient","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Same infant biomarker comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Maternal 0, 1.2, 2.4 or 10 mg/day thiamine from weeks 2-24 postpartum; study exposures only.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay response is not a contradiction: endpoints differ. The study did not establish a universal clinical threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The concentration marker and functional assay did not identify identical dose responses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b1-gallant2021] Low-dose thiamine supplementation of lactating Cambodian mothers improves human milk thiamine concentrations: a randomized controlled trial (2021). https://pubmed.ncbi.nlm.nih.gov/33829271/ DOI: 10.1093/ajcn/nqab052","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Infant blood","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"847673d8-cc95-5355-bf50-49042fc78111","evidence_kind":"source_excerpt","locator":"Lines 1831-1841","start_line":1831,"end_line":1841,"excerpt":"### b1-milk-trial-marker-difference\nInfant ETK activation coefficient differed significantly only between the highest maternal-dose group and placebo, whereas infant ThDP differed for all active groups.\nCondition category: biomarker_context\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The concentration marker and functional assay did not identify identical dose responses.\norganism: Homo sapiens\ntissue_or_cell_type: Infant blood\nexperimental_model: Same infant biomarker comparison.\nlimitations: Assay response is not a contradiction: endpoints differ. The study did not establish a universal clinical threshold.\nexposure: Maternal 0, 1.2, 2.4 or 10 mg/day thiamine from weeks 2-24 postpartum; study exposures only.\n[b1-gallant2021] Low-dose thiamine supplementation of lactating Cambodian mothers improves human milk thiamine concentrations: a randomized controlled trial (2021). https://pubmed.ncbi.nlm.nih.gov/33829271/ DOI: 10.1093/ajcn/nqab052","model_system":"Same infant biomarker comparison.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b1-gallant2021] Low-dose thiamine supplementation of lactating Cambodian mothers improves human milk thiamine concentrations: a randomized controlled trial (2021). https://pubmed.ncbi.nlm.nih.gov/33829271/ DOI: 10.1093/ajcn/nqab052","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}