Component
Human microsomal cytochrome b5 / CYB5A
Human microsomal cytochrome b5 / CYB5A. Species, exposure and limitations are retained in each linked claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
CYB5A siRNA in HEK293 cells and Cyb5a knockout in mice did not support a required role for the microsomal isoform in measured N-reduction.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/molybdenum-research/23703616.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "9034da6c790ca6e0c60dd765eec631ea2875c6caf65f6d15d59733bc5e0ee0ec", "start_char": 0, "end_char": 1596, "text_sha256": "9034da6c790ca6e0c60dd765eec631ea2875c6caf65f6d15d59733bc5e0ee0ec"}
- experimental_model
- Human-cell siRNA plus recombinant cytochrome-b5/cofactor reconstitution
- exposure
- mARC, CYB5B and CYB5A depletion; apo-CYB5
- limitations
- Expression-dependent cellular contributions; no clinical iron or B2 supplementation test.
- nutrient_topic
- Molybdenum research collection; topical membership is not evidence of a direct dietary effect. · Molybdenum
- organism
- Homo sapiens; separate mouse Cyb5a knockout
- plain_language
- The location and identity of the cytochrome b5 partner matter.
- primary_references
- [mo-p23703616] The involvement of mitochondrial amidoxime reducing components 1 and 2 and mitochondrial cytochrome b5 in N-reductive metabolism in human cells. (2013). https://pubmed.ncbi.nlm.nih.gov/23703616/ DOI: 10.1074/jbc.m113.474916
- tissue_or_cell_type
- HEK293 and second human cell line; purified proteins
Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17) · lines 1015–1026
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human-cell siRNA plus recombinant cytochrome-b5/cofactor reconstitution · source_derived_draft · unverified_draft
### mo-cyb5a-not-substitute CYB5A siRNA in HEK293 cells and Cyb5a knockout in mice did not support a required role for the microsomal isoform in measured N-reduction. Condition category: normal nutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect. plain_language: The location and identity of the cytochrome b5 partner matter. organism: Homo sapiens; separate mouse Cyb5a knockout tissue_or_cell_type: HEK293 and second human cell line; purified proteins experimental_model: Human-cell siRNA plus recombinant cytochrome-b5/cofactor reconstitution limitations: Expression-dependent cellular contributions; no clinical iron or B2 supplementation test. exposure: mARC, CYB5B and CYB5A depletion; apo-CYB5 evidence_span: {"source_cache": "artifacts/molybdenum-research/23703616.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "9034da6c790ca6e0c60dd765eec631ea2875c6caf65f6d15d59733bc5e0ee0ec", "start_char": 0, "end_char": 1596, "text_sha256": "9034da6c790ca6e0c60dd765eec631ea2875c6caf65f6d15d59733bc5e0ee0ec"} [mo-p23703616] The involvement of mitochondrial amidoxime reducing components 1 and 2 and mitochondrial cytochrome b5 in N-reductive metabolism in human cells. (2013). https://pubmed.ncbi.nlm.nih.gov/23703616/ DOI: 10.1074/jbc.m113.474916
Complete structured claim and evidence
Where it participates (unsigned role)
Reconstituting the non-heme iron and heme sites of a human SCD/cytochrome-b5 proteoliposome complex restored conversion of stearoyl-CoA to oleoyl-CoA.
Experimental context and source evidence
- evidence_access
- Primary abstract
- experimental_model
- Human proteins translated in wheat-germ extract and assembled in liposomes.
- limitations
- The tested substrate was stearoyl-CoA, not vaccenic acid; nutrient status was not tested.
- nutrient_topic
- CLA collection; isomer, preparation, species, exposure and manipulation remain explicit. · Conjugated linoleic acid / CLA isomer family
- plain_language
- The desaturation machinery needs metal-containing partners.
- primary_references
- Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18765284/ · DOI 10.1016/j.pep.2008.08.002
Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19) · lines 46–52
AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text. · supports · Human proteins translated in wheat-germ extract and assembled in liposomes. · source_derived_draft · unverified_draft
## cla-scd-cofactors The desaturation machinery needs metal-containing partners. Reconstituting the non-heme iron and heme sites of a human SCD/cytochrome-b5 proteoliposome complex restored conversion of stearoyl-CoA to oleoyl-CoA. Model: Human proteins translated in wheat-germ extract and assembled in liposomes. Limitations: The tested substrate was stearoyl-CoA, not vaccenic acid; nutrient status was not tested. Evidence access: Primary abstract Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18765284/ · DOI 10.1016/j.pep.2008.08.002
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.