{"id":"ad86c5df-bb0f-5643-b35b-7e22b8244bd7","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-scd-cofactors","predicate":"catalyzes","statement":"Reconstituting the non-heme iron and heme sites of a human SCD/cytochrome-b5 proteoliposome complex restored conversion of stearoyl-CoA to oleoyl-CoA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"aa0c8e56-cd27-50d9-a30b-903eb1dc87f6","mechanism_event_label":"The desaturation machinery needs metal-containing partners.","subject":{"id":"ff05052c-a102-5f63-bc9b-66a7a632ebf3","slug":"scd","display_name":"Human stearoyl-CoA desaturase / SCD","entity_type_key":"protein"},"object":{"id":"17d896f6-1eb0-5320-af84-d4251343df41","slug":"oleoyl-coa","display_name":"Oleoyl-CoA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"aa0c8e56-cd27-50d9-a30b-903eb1dc87f6","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-scd-cofactors-event","event_type":"observed_relationship","label":"The desaturation machinery needs metal-containing partners.","description":"Reconstituting the non-heme iron and heme sites of a human SCD/cytochrome-b5 proteoliposome complex restored conversion of stearoyl-CoA to oleoyl-CoA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ff05052c-a102-5f63-bc9b-66a7a632ebf3","slug":"scd","display_name":"Human stearoyl-CoA desaturase / SCD","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"17d896f6-1eb0-5320-af84-d4251343df41","slug":"oleoyl-coa","display_name":"Oleoyl-CoA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"98d55cd7-2068-56df-8911-496b37a87f47","slug":"cyb5a","display_name":"Human microsomal cytochrome b5 / CYB5A","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"59d6d1cd-df32-5b58-b950-3188bc7b95d6","slug":"iron-ii","display_name":"Ferrous iron","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2e1f7e0a-8b54-5ea3-9d34-01e7167f9097","slug":"heme","display_name":"Heme","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"c74257f2-4edf-593a-b1ff-238429e967f7","slug":"stearoyl-coa","display_name":"Stearoyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human proteins translated in wheat-germ extract and assembled in liposomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The tested substrate was stearoyl-CoA, not vaccenic acid; nutrient status was not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The desaturation machinery needs metal-containing partners.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18765284/ · DOI 10.1016/j.pep.2008.08.002","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5ff88407-b269-514e-b84e-eb1caea68d86","evidence_kind":"source_excerpt","locator":"Lines 46-52","start_line":46,"end_line":52,"excerpt":"## cla-scd-cofactors\nThe desaturation machinery needs metal-containing partners.\nReconstituting the non-heme iron and heme sites of a human SCD/cytochrome-b5 proteoliposome complex restored conversion of stearoyl-CoA to oleoyl-CoA.\nModel: Human proteins translated in wheat-germ extract and assembled in liposomes.\nLimitations: The tested substrate was stearoyl-CoA, not vaccenic acid; nutrient status was not tested.\nEvidence access: Primary abstract\nWheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18765284/ · DOI 10.1016/j.pep.2008.08.002","model_system":"Human proteins translated in wheat-germ extract and assembled in liposomes.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f9fe73a8a4008d52061c65abe6de7a9806da75f1c478c5adace9387b8ffaad9e","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}