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(2013). https://pubmed.ncbi.nlm.nih.gov/23703616/ DOI: 10.1074/jbc.m113.474916","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293 and second human cell line; purified proteins","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3c5b26ab-0c04-59e5-ad3e-1fa8d3465752","evidence_kind":"source_excerpt","locator":"Lines 1015-1026","start_line":1015,"end_line":1026,"excerpt":"### mo-cyb5a-not-substitute\nCYB5A siRNA in HEK293 cells and Cyb5a knockout in mice did not support a required role for the microsomal isoform in measured N-reduction.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The location and identity of the cytochrome b5 partner matter.\norganism: Homo sapiens; separate mouse Cyb5a knockout\ntissue_or_cell_type: HEK293 and second human cell line; purified proteins\nexperimental_model: Human-cell siRNA plus recombinant cytochrome-b5/cofactor reconstitution\nlimitations: Expression-dependent cellular contributions; no clinical iron or B2 supplementation test.\nexposure: mARC, CYB5B and CYB5A depletion; apo-CYB5\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/23703616.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9034da6c790ca6e0c60dd765eec631ea2875c6caf65f6d15d59733bc5e0ee0ec\", \"start_char\": 0, \"end_char\": 1596, \"text_sha256\": \"9034da6c790ca6e0c60dd765eec631ea2875c6caf65f6d15d59733bc5e0ee0ec\"}\n[mo-p23703616] The involvement of mitochondrial amidoxime reducing components 1 and 2 and mitochondrial cytochrome b5 in N-reductive metabolism in human cells. 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