Component

Mammalian ClC-5 chloride/proton exchanger

Mammalian ClC-5 chloride/proton exchanger. Species, exposure and limitations are retained in each linked claim.

2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. ClC-5 transported protons against their electrochemical gradient in coupling with anion transport.

    Mammalian ClC-5 chloride/proton exchanger → Coupled chloride/proton exchange source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/chloride-research/16034421.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "3a437e5233f3bb917ef5bbfee37e6eaed1745baf86fd4f35c85d8ad509dfa0f0", "start_char": 0, "end_char": 1359, "text_sha256": "3a437e5233f3bb917ef5bbfee37e6eaed1745baf86fd4f35c85d8ad509dfa0f0"}
    experimental_model
    Voltage and proton-flux measurements
    exposure
    Positive voltage and pore-glutamate mutation
    limitations
    Heterologous measurements; intracellular physiological direction depends on gradients.
    nutrient_topic
    Chloride research collection; topical membership is not evidence of a direct dietary effect. · Chloride
    organism
    Mammalian ClC constructs in expression systems
    plain_language
    Some proteins historically called chloride channels are coupled chloride/proton exchangers.
    primary_references
    [chloride-p16034421] Chloride/proton antiporter activity of mammalian CLC proteins ClC-4 and ClC-5. (2005). https://pubmed.ncbi.nlm.nih.gov/16034421/ DOI: 10.1038/nature03720
    tissue_or_cell_type
    Plasma-membrane-expressed transport proteins

    Chloride: transport, acid-base balance, nutrient interactions and loss states (2026-09-17) · lines 640–651

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Voltage and proton-flux measurements · source_derived_draft · unverified_draft

    ### chloride-clc5-exchange ClC-5 transported protons against their electrochemical gradient in coupling with anion transport. Condition category: normal nutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect. plain_language: Some proteins historically called chloride channels are coupled chloride/proton exchangers. organism: Mammalian ClC constructs in expression systems tissue_or_cell_type: Plasma-membrane-expressed transport proteins experimental_model: Voltage and proton-flux measurements limitations: Heterologous measurements; intracellular physiological direction depends on gradients. exposure: Positive voltage and pore-glutamate mutation evidence_span: {"source_cache": "artifacts/chloride-research/16034421.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "3a437e5233f3bb917ef5bbfee37e6eaed1745baf86fd4f35c85d8ad509dfa0f0", "start_char": 0, "end_char": 1359, "text_sha256": "3a437e5233f3bb917ef5bbfee37e6eaed1745baf86fd4f35c85d8ad509dfa0f0"} [chloride-p16034421] Chloride/proton antiporter activity of mammalian CLC proteins ClC-4 and ClC-5. (2005). https://pubmed.ncbi.nlm.nih.gov/16034421/ DOI: 10.1038/nature03720
    Complete structured claim and evidence

  2. Measured ClC-5 transport stoichiometry was two chloride ions per proton.

    Mammalian ClC-5 chloride/proton exchanger → Chloride ion source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/chloride-research/19131966.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "04e75eb135b2ed1c3a14a69b20f602132fc4a55cc0e1d3f6a11ee2834692a79e", "start_char": 0, "end_char": 1161, "text_sha256": "04e75eb135b2ed1c3a14a69b20f602132fc4a55cc0e1d3f6a11ee2834692a79e"}
    experimental_model
    Absolute proton-flux imaging
    exposure
    Proton gradients and S168 substitutions
    limitations
    Assay stoichiometry, not dietary requirements.
    nutrient_topic
    Chloride research collection; topical membership is not evidence of a direct dietary effect. · Chloride
    organism
    Mammalian ClC-5 in Xenopus oocytes
    plain_language
    This protein couples chloride transport to proton movement at a measured ratio.
    primary_references
    [chloride-p19131966] Conversion of the 2 Cl(-)/1 H+ antiporter ClC-5 in a NO3(-)/H+ antiporter by a single point mutation. (2009). https://pubmed.ncbi.nlm.nih.gov/19131966/ DOI: 10.1038/emboj.2008.284
    tissue_or_cell_type
    Expressed plasma membrane

    Chloride: transport, acid-base balance, nutrient interactions and loss states (2026-09-17) · lines 666–677

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Absolute proton-flux imaging · source_derived_draft · unverified_draft

    ### chloride-clc5-stoichiometry Measured ClC-5 transport stoichiometry was two chloride ions per proton. Condition category: normal nutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect. plain_language: This protein couples chloride transport to proton movement at a measured ratio. organism: Mammalian ClC-5 in Xenopus oocytes tissue_or_cell_type: Expressed plasma membrane experimental_model: Absolute proton-flux imaging limitations: Assay stoichiometry, not dietary requirements. exposure: Proton gradients and S168 substitutions evidence_span: {"source_cache": "artifacts/chloride-research/19131966.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "04e75eb135b2ed1c3a14a69b20f602132fc4a55cc0e1d3f6a11ee2834692a79e", "start_char": 0, "end_char": 1161, "text_sha256": "04e75eb135b2ed1c3a14a69b20f602132fc4a55cc0e1d3f6a11ee2834692a79e"} [chloride-p19131966] Conversion of the 2 Cl(-)/1 H+ antiporter ClC-5 in a NO3(-)/H+ antiporter by a single point mutation. (2009). https://pubmed.ncbi.nlm.nih.gov/19131966/ DOI: 10.1038/emboj.2008.284
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards