{"id":"0d78415c-640c-58e5-b1b1-a65d5880e3ff","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-clc5-stoichiometry","predicate":"exchanges","statement":"Measured ClC-5 transport stoichiometry was two chloride ions per proton.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4d56ad3d-4b8d-5316-8fa7-f95cb3df3027","mechanism_event_label":"This protein couples chloride transport to proton movement at a measured ratio.","subject":{"id":"05641ccc-12af-505c-9207-db1339b3208b","slug":"clcn5","display_name":"Mammalian ClC-5 chloride/proton exchanger","entity_type_key":"protein"},"object":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"4d56ad3d-4b8d-5316-8fa7-f95cb3df3027","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-clc5-stoichiometry-event","event_type":"biochemical_relationship","label":"This protein couples chloride transport to proton movement at a measured ratio.","description":"Measured ClC-5 transport stoichiometry was two chloride ions per proton.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"05641ccc-12af-505c-9207-db1339b3208b","slug":"clcn5","display_name":"Mammalian ClC-5 chloride/proton exchanger","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/19131966.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"04e75eb135b2ed1c3a14a69b20f602132fc4a55cc0e1d3f6a11ee2834692a79e\", \"start_char\": 0, \"end_char\": 1161, \"text_sha256\": \"04e75eb135b2ed1c3a14a69b20f602132fc4a55cc0e1d3f6a11ee2834692a79e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Absolute proton-flux imaging","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Proton gradients and S168 substitutions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay stoichiometry, not dietary requirements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mammalian ClC-5 in Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This protein couples chloride transport to proton movement at a measured ratio.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p19131966] Conversion of the 2 Cl(-)/1 H+ antiporter ClC-5 in a NO3(-)/H+ antiporter by a single point mutation. (2009). https://pubmed.ncbi.nlm.nih.gov/19131966/ DOI: 10.1038/emboj.2008.284","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Expressed plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"de1abd7c-80f0-5475-ba7a-400574d4c3b8","evidence_kind":"source_excerpt","locator":"Lines 666-677","start_line":666,"end_line":677,"excerpt":"### chloride-clc5-stoichiometry\nMeasured ClC-5 transport stoichiometry was two chloride ions per proton.\nCondition category: normal\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This protein couples chloride transport to proton movement at a measured ratio.\norganism: Mammalian ClC-5 in Xenopus oocytes\ntissue_or_cell_type: Expressed plasma membrane\nexperimental_model: Absolute proton-flux imaging\nlimitations: Assay stoichiometry, not dietary requirements.\nexposure: Proton gradients and S168 substitutions\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/19131966.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"04e75eb135b2ed1c3a14a69b20f602132fc4a55cc0e1d3f6a11ee2834692a79e\", \"start_char\": 0, \"end_char\": 1161, \"text_sha256\": \"04e75eb135b2ed1c3a14a69b20f602132fc4a55cc0e1d3f6a11ee2834692a79e\"}\n[chloride-p19131966] Conversion of the 2 Cl(-)/1 H+ antiporter ClC-5 in a NO3(-)/H+ antiporter by a single point mutation. 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