{"id":"015ebc53-868c-578d-89cc-4c2c2981fff0","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-clc5-exchange","predicate":"mediates","statement":"ClC-5 transported protons against their electrochemical gradient in coupling with anion transport.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3dc76010-5d8c-5002-8878-c6b9279f1be9","mechanism_event_label":"Some proteins historically called chloride channels are coupled chloride/proton exchangers.","subject":{"id":"05641ccc-12af-505c-9207-db1339b3208b","slug":"clcn5","display_name":"Mammalian ClC-5 chloride/proton exchanger","entity_type_key":"protein"},"object":{"id":"90606865-2992-5388-baff-9b101da2b8b0","slug":"chloride-proton-exchange","display_name":"Coupled chloride/proton exchange","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3dc76010-5d8c-5002-8878-c6b9279f1be9","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-clc5-exchange-event","event_type":"biochemical_relationship","label":"Some proteins historically called chloride channels are coupled chloride/proton exchangers.","description":"ClC-5 transported protons against their electrochemical gradient in coupling with anion transport.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"transported_anion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"05641ccc-12af-505c-9207-db1339b3208b","slug":"clcn5","display_name":"Mammalian ClC-5 chloride/proton exchanger","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"90606865-2992-5388-baff-9b101da2b8b0","slug":"chloride-proton-exchange","display_name":"Coupled chloride/proton exchange","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/16034421.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3a437e5233f3bb917ef5bbfee37e6eaed1745baf86fd4f35c85d8ad509dfa0f0\", \"start_char\": 0, \"end_char\": 1359, \"text_sha256\": \"3a437e5233f3bb917ef5bbfee37e6eaed1745baf86fd4f35c85d8ad509dfa0f0\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Voltage and proton-flux measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Positive voltage and pore-glutamate mutation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Heterologous measurements; intracellular physiological direction depends on gradients.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mammalian ClC constructs in expression systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Some proteins historically called chloride channels are coupled chloride/proton exchangers.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p16034421] Chloride/proton antiporter activity of mammalian CLC proteins ClC-4 and ClC-5. (2005). https://pubmed.ncbi.nlm.nih.gov/16034421/ DOI: 10.1038/nature03720","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Plasma-membrane-expressed transport proteins","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e887580c-3955-55fc-b3fb-007e364df456","evidence_kind":"source_excerpt","locator":"Lines 640-651","start_line":640,"end_line":651,"excerpt":"### chloride-clc5-exchange\nClC-5 transported protons against their electrochemical gradient in coupling with anion transport.\nCondition category: normal\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Some proteins historically called chloride channels are coupled chloride/proton exchangers.\norganism: Mammalian ClC constructs in expression systems\ntissue_or_cell_type: Plasma-membrane-expressed transport proteins\nexperimental_model: Voltage and proton-flux measurements\nlimitations: Heterologous measurements; intracellular physiological direction depends on gradients.\nexposure: Positive voltage and pore-glutamate mutation\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/16034421.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3a437e5233f3bb917ef5bbfee37e6eaed1745baf86fd4f35c85d8ad509dfa0f0\", \"start_char\": 0, \"end_char\": 1359, \"text_sha256\": \"3a437e5233f3bb917ef5bbfee37e6eaed1745baf86fd4f35c85d8ad509dfa0f0\"}\n[chloride-p16034421] Chloride/proton antiporter activity of mammalian CLC proteins ClC-4 and ClC-5. 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