Component

Sulforaphane plus selenite, study-specific combination

Sulforaphane plus selenite, study-specific combination. Species, exposure and limitations are retained in each linked claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. Combined sulforaphane and selenite protected HepG2 cells against paraquat-induced death, whereas either alone had little or no protection.

    Sulforaphane plus selenite, study-specific combination → HepG2 survival after paraquat challenge source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/sulforaphane-research/12663510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "95f1de031ede41aa12f975dff28193dcb426465d4bda94bb84d4e60ef6c292b9", "start_char": 0, "end_char": 1535, "text_sha256": "95f1de031ede41aa12f975dff28193dcb426465d4bda94bb84d4e60ef6c292b9"}
    experimental_model
    Transcription/translation and inhibitor experiments
    exposure
    Sulforaphane, sodium selenite, combined treatment and pathway inhibitors
    limitations
    Cell-culture synergy at specified exposures is not a clinical supplement recommendation; TXNRD1 and TXNRD2 were not interchangeable.
    nutrient_topic
    Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
    organism
    Human HepG2 hepatoma cells
    plain_language
    A measured combination effect occurred in this specific injury model.
    primary_references
    [sulforaphane-p12663510] Synergy between sulforaphane and selenium in the induction of thioredoxin reductase 1 requires both transcriptional and translational modulation. (2003). https://pubmed.ncbi.nlm.nih.gov/12663510/ DOI: 10.1093/carcin/24.3.497
    tissue_or_cell_type
    Thioredoxin reductase regulation

    Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 749–760

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Transcription/translation and inhibitor experiments · source_derived_draft · unverified_draft

    ### sulforaphane-paraquat-protection Combined sulforaphane and selenite protected HepG2 cells against paraquat-induced death, whereas either alone had little or no protection. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: A measured combination effect occurred in this specific injury model. organism: Human HepG2 hepatoma cells tissue_or_cell_type: Thioredoxin reductase regulation experimental_model: Transcription/translation and inhibitor experiments limitations: Cell-culture synergy at specified exposures is not a clinical supplement recommendation; TXNRD1 and TXNRD2 were not interchangeable. exposure: Sulforaphane, sodium selenite, combined treatment and pathway inhibitors evidence_span: {"source_cache": "artifacts/sulforaphane-research/12663510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "95f1de031ede41aa12f975dff28193dcb426465d4bda94bb84d4e60ef6c292b9", "start_char": 0, "end_char": 1535, "text_sha256": "95f1de031ede41aa12f975dff28193dcb426465d4bda94bb84d4e60ef6c292b9"} [sulforaphane-p12663510] Synergy between sulforaphane and selenium in the induction of thioredoxin reductase 1 requires both transcriptional and translational modulation. (2003). https://pubmed.ncbi.nlm.nih.gov/12663510/ DOI: 10.1093/carcin/24.3.497
    Complete structured claim and evidence

  2. Combined treatment increased TXNRD1 protein 5.5-fold, activity 13-fold and mRNA 6.5-fold in HepG2 cells.

    Sulforaphane plus selenite, study-specific combination → Human TXNRD1 enzyme activity source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/sulforaphane-research/12663510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "95f1de031ede41aa12f975dff28193dcb426465d4bda94bb84d4e60ef6c292b9", "start_char": 0, "end_char": 1535, "text_sha256": "95f1de031ede41aa12f975dff28193dcb426465d4bda94bb84d4e60ef6c292b9"}
    experimental_model
    Transcription/translation and inhibitor experiments
    exposure
    Sulforaphane, sodium selenite, combined treatment and pathway inhibitors
    limitations
    Cell-culture synergy at specified exposures is not a clinical supplement recommendation; TXNRD1 and TXNRD2 were not interchangeable.
    nutrient_topic
    Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
    organism
    Human HepG2 hepatoma cells
    plain_language
    Gene expression and enzyme production can combine to produce a larger response.
    primary_references
    [sulforaphane-p12663510] Synergy between sulforaphane and selenium in the induction of thioredoxin reductase 1 requires both transcriptional and translational modulation. (2003). https://pubmed.ncbi.nlm.nih.gov/12663510/ DOI: 10.1093/carcin/24.3.497
    tissue_or_cell_type
    Thioredoxin reductase regulation

    Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 736–747

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Transcription/translation and inhibitor experiments · source_derived_draft · unverified_draft

    ### sulforaphane-selenium-sfn-synergy Combined treatment increased TXNRD1 protein 5.5-fold, activity 13-fold and mRNA 6.5-fold in HepG2 cells. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: Gene expression and enzyme production can combine to produce a larger response. organism: Human HepG2 hepatoma cells tissue_or_cell_type: Thioredoxin reductase regulation experimental_model: Transcription/translation and inhibitor experiments limitations: Cell-culture synergy at specified exposures is not a clinical supplement recommendation; TXNRD1 and TXNRD2 were not interchangeable. exposure: Sulforaphane, sodium selenite, combined treatment and pathway inhibitors evidence_span: {"source_cache": "artifacts/sulforaphane-research/12663510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "95f1de031ede41aa12f975dff28193dcb426465d4bda94bb84d4e60ef6c292b9", "start_char": 0, "end_char": 1535, "text_sha256": "95f1de031ede41aa12f975dff28193dcb426465d4bda94bb84d4e60ef6c292b9"} [sulforaphane-p12663510] Synergy between sulforaphane and selenium in the induction of thioredoxin reductase 1 requires both transcriptional and translational modulation. (2003). https://pubmed.ncbi.nlm.nih.gov/12663510/ DOI: 10.1093/carcin/24.3.497
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. Nrf2 knockdown lowered survival under combined treatment from 82.4% to 51.1%.

    Experimental human NFE2L2 knockdown → Survival after peroxide challenge, model specified source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/sulforaphane-research/26094214.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e", "start_char": 0, "end_char": 1568, "text_sha256": "de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e"}
    experimental_model
    Combination treatment and siRNA loss-of-function
    exposure
    Sulforaphane plus selenium with Nrf2 or TXNRD1 knockdown
    limitations
    One cell model; survival percentages are assay-specific and not clinical benefit estimates.
    nutrient_topic
    Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
    organism
    Human nontransformed CCD841 colonic cells
    plain_language
    The regulator was necessary for much of the observed protection.
    primary_references
    [sulforaphane-p26094214] Synergy between sulforaphane and selenium in protection against oxidative damage in colonic CCD841 cells. (2015). https://pubmed.ncbi.nlm.nih.gov/26094214/ DOI: 10.1016/j.nutres.2015.05.011
    tissue_or_cell_type
    Nrf2/TXNRD1 and peroxide survival
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 788–799

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Combination treatment and siRNA loss-of-function · source_derived_draft · unverified_draft

    ### sulforaphane-nrf2-knockdown Nrf2 knockdown lowered survival under combined treatment from 82.4% to 51.1%. Condition category: machinery_impairment nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: The regulator was necessary for much of the observed protection. organism: Human nontransformed CCD841 colonic cells tissue_or_cell_type: Nrf2/TXNRD1 and peroxide survival experimental_model: Combination treatment and siRNA loss-of-function limitations: One cell model; survival percentages are assay-specific and not clinical benefit estimates. exposure: Sulforaphane plus selenium with Nrf2 or TXNRD1 knockdown evidence_span: {"source_cache": "artifacts/sulforaphane-research/26094214.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e", "start_char": 0, "end_char": 1568, "text_sha256": "de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e"} [sulforaphane-p26094214] Synergy between sulforaphane and selenium in protection against oxidative damage in colonic CCD841 cells. (2015). https://pubmed.ncbi.nlm.nih.gov/26094214/ DOI: 10.1016/j.nutres.2015.05.011
    Complete structured claim and evidence

  2. TXNRD1 knockdown lowered survival under combined treatment from 82.4% to 66.5% in the peroxide-challenge assay.

    Experimental human TXNRD1 knockdown → Survival after peroxide challenge, model specified source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/sulforaphane-research/26094214.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e", "start_char": 0, "end_char": 1568, "text_sha256": "de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e"}
    experimental_model
    Combination treatment and siRNA loss-of-function
    exposure
    Sulforaphane plus selenium with Nrf2 or TXNRD1 knockdown
    limitations
    One cell model; survival percentages are assay-specific and not clinical benefit estimates.
    nutrient_topic
    Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
    organism
    Human nontransformed CCD841 colonic cells
    plain_language
    Increasing the signal cannot replace the enzyme that carries out part of the response.
    primary_references
    [sulforaphane-p26094214] Synergy between sulforaphane and selenium in protection against oxidative damage in colonic CCD841 cells. (2015). https://pubmed.ncbi.nlm.nih.gov/26094214/ DOI: 10.1016/j.nutres.2015.05.011
    tissue_or_cell_type
    Nrf2/TXNRD1 and peroxide survival
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 775–786

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Combination treatment and siRNA loss-of-function · source_derived_draft · unverified_draft

    ### sulforaphane-txnrd1-knockdown TXNRD1 knockdown lowered survival under combined treatment from 82.4% to 66.5% in the peroxide-challenge assay. Condition category: machinery_impairment nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: Increasing the signal cannot replace the enzyme that carries out part of the response. organism: Human nontransformed CCD841 colonic cells tissue_or_cell_type: Nrf2/TXNRD1 and peroxide survival experimental_model: Combination treatment and siRNA loss-of-function limitations: One cell model; survival percentages are assay-specific and not clinical benefit estimates. exposure: Sulforaphane plus selenium with Nrf2 or TXNRD1 knockdown evidence_span: {"source_cache": "artifacts/sulforaphane-research/26094214.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e", "start_char": 0, "end_char": 1568, "text_sha256": "de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e"} [sulforaphane-p26094214] Synergy between sulforaphane and selenium in protection against oxidative damage in colonic CCD841 cells. (2015). https://pubmed.ncbi.nlm.nih.gov/26094214/ DOI: 10.1016/j.nutres.2015.05.011
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards