{"id":"3e66de4f-32f1-5d80-856e-6546f314f867","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-nrf2-knockdown","predicate":"reduced","statement":"Nrf2 knockdown lowered survival under combined treatment from 82.4% to 51.1%.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"88c48f50-8e19-5eb3-83ec-64c418a5cd8d","mechanism_event_label":"The regulator was necessary for much of the observed protection.","subject":{"id":"bc697dff-6755-5447-9db1-e714fef23a7b","slug":"nfe2l2-knockdown","display_name":"Experimental human NFE2L2 knockdown","entity_type_key":"protein_state"},"object":{"id":"ae6e13eb-3a29-5c90-83b1-dc61bb332584","slug":"peroxide-challenged-cell-survival","display_name":"Survival after peroxide challenge, model specified","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"88c48f50-8e19-5eb3-83ec-64c418a5cd8d","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-nrf2-knockdown-event","event_type":"biochemical_relationship","label":"The regulator was necessary for much of the observed protection.","description":"Nrf2 knockdown lowered survival under combined treatment from 82.4% to 51.1%.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"685a1738-6d9f-54e6-a82e-bd5b01adc9f1","slug":"sulforaphane-selenite-combination","display_name":"Sulforaphane plus selenite, study-specific combination","entity_type_key":"chemical_species"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"64fac263-c4d5-51c8-beba-d061868b0ce2","slug":"txnrd1","display_name":"TXNRD1","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"regulator","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bc697dff-6755-5447-9db1-e714fef23a7b","slug":"nfe2l2-knockdown","display_name":"Experimental human NFE2L2 knockdown","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ae6e13eb-3a29-5c90-83b1-dc61bb332584","slug":"peroxide-challenged-cell-survival","display_name":"Survival after peroxide challenge, model specified","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/26094214.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e\", \"start_char\": 0, \"end_char\": 1568, \"text_sha256\": \"de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Combination treatment and siRNA loss-of-function","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sulforaphane plus selenium with Nrf2 or TXNRD1 knockdown","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"One cell model; survival percentages are assay-specific and not clinical benefit estimates.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human nontransformed CCD841 colonic cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The regulator was necessary for much of the observed protection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p26094214] Synergy between sulforaphane and selenium in protection against oxidative damage in colonic CCD841 cells. (2015). https://pubmed.ncbi.nlm.nih.gov/26094214/ DOI: 10.1016/j.nutres.2015.05.011","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Nrf2/TXNRD1 and peroxide survival","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"e9206184-2ca8-57f1-898b-1ccd897aeb83","evidence_kind":"source_excerpt","locator":"Lines 788-799","start_line":788,"end_line":799,"excerpt":"### sulforaphane-nrf2-knockdown\nNrf2 knockdown lowered survival under combined treatment from 82.4% to 51.1%.\nCondition category: machinery_impairment\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The regulator was necessary for much of the observed protection.\norganism: Human nontransformed CCD841 colonic cells\ntissue_or_cell_type: Nrf2/TXNRD1 and peroxide survival\nexperimental_model: Combination treatment and siRNA loss-of-function\nlimitations: One cell model; survival percentages are assay-specific and not clinical benefit estimates.\nexposure: Sulforaphane plus selenium with Nrf2 or TXNRD1 knockdown\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/26094214.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e\", \"start_char\": 0, \"end_char\": 1568, \"text_sha256\": \"de55004b06332873c312c03a18cec65eeb22c1bab6daddf5cde8a7977817567e\"}\n[sulforaphane-p26094214] Synergy between sulforaphane and selenium in protection against oxidative damage in colonic CCD841 cells. (2015). https://pubmed.ncbi.nlm.nih.gov/26094214/ DOI: 10.1016/j.nutres.2015.05.011","model_system":"Combination treatment and siRNA loss-of-function","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sulforaphane-p26094214] Synergy between sulforaphane and selenium in protection against oxidative damage in colonic CCD841 cells. (2015). https://pubmed.ncbi.nlm.nih.gov/26094214/ DOI: 10.1016/j.nutres.2015.05.011","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"a4bafbf7-f117-53c9-a6d7-8fb25e4aca72","stable_key":"import-44eaeae5-557a-552b-8998-884f30462e2a","title":"Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f355c492a6c35673d8260b1328667fe206e5315b9fcaa5aeff53d80373c7ef97","revision_id":"f2c47d71-34cd-5160-8e92-fe196b576707","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}