Component

Sulforaphane-N-acetylcysteine conjugate / SFN-NAC

Sulforaphane-N-acetylcysteine conjugate / SFN-NAC. Species, exposure and limitations are retained in each linked claim.

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. SFN-NAC also inhibited HDAC activity in the in vitro comparison.

    Sulforaphane-N-acetylcysteine conjugate / SFN-NAC → histone deacetylase activity source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/sulforaphane-research/15313918.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e", "start_char": 0, "end_char": 1861, "text_sha256": "9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e"}
    experimental_model
    Cell exposure, enzyme assays, metabolites and chromatin immunoprecipitation
    exposure
    Parent SFN, SFN-GSH, SFN-Cys and SFN-NAC; GST inhibition
    limitations
    High-level HDAC activity was measured without establishing universal isoform inhibition; cell effects are not cancer-treatment outcomes.
    nutrient_topic
    Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
    organism
    Human HEK293 and HCT116 cells
    plain_language
    Another separately tracked conjugate affected this enzyme readout.
    primary_references
    [sulforaphane-p15313918] A novel mechanism of chemoprotection by sulforaphane: inhibition of histone deacetylase. (2004). https://pubmed.ncbi.nlm.nih.gov/15313918/ DOI: 10.1158/0008-5472.can-04-1326
    tissue_or_cell_type
    Histone deacetylase activity and histone acetylation

    Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 580–591

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Cell exposure, enzyme assays, metabolites and chromatin immunoprecipitation · source_derived_draft · unverified_draft

    ### sulforaphane-nac-hdac SFN-NAC also inhibited HDAC activity in the in vitro comparison. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: Another separately tracked conjugate affected this enzyme readout. organism: Human HEK293 and HCT116 cells tissue_or_cell_type: Histone deacetylase activity and histone acetylation experimental_model: Cell exposure, enzyme assays, metabolites and chromatin immunoprecipitation limitations: High-level HDAC activity was measured without establishing universal isoform inhibition; cell effects are not cancer-treatment outcomes. exposure: Parent SFN, SFN-GSH, SFN-Cys and SFN-NAC; GST inhibition evidence_span: {"source_cache": "artifacts/sulforaphane-research/15313918.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e", "start_char": 0, "end_char": 1861, "text_sha256": "9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e"} [sulforaphane-p15313918] A novel mechanism of chemoprotection by sulforaphane: inhibition of histone deacetylase. (2004). https://pubmed.ncbi.nlm.nih.gov/15313918/ DOI: 10.1158/0008-5472.can-04-1326
    Complete structured claim and evidence

What acts on it

  1. Sulforaphane-N-acetylcysteine was separately quantified in human urine after broccoli sprout exposure.

    Sulforaphane / SFN, stereochemistry specified per study → Sulforaphane-N-acetylcysteine conjugate / SFN-NAC source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/sulforaphane-research/18729326.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3", "start_char": 0, "end_char": 2172, "text_sha256": "1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3"}
    experimental_model
    Isotope-dilution LC-MS/MS assay validation in intervention urines
    exposure
    Broccoli sprout intervention specimens with equivalent administered preparation
    limitations
    Analytical exposure study; metabolite concentration is not tissue efficacy and does not identify each responsible human enzyme.
    nutrient_topic
    Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
    organism
    Human, 98 urine specimens
    plain_language
    This metabolite has its own identity and can be measured separately from the parent.
    primary_references
    [sulforaphane-p18729326] Quantification of sulforaphane mercapturic acid pathway conjugates in human urine by high-performance liquid chromatography and isotope-dilution tandem mass spectrometry. (2008). https://pubmed.ncbi.nlm.nih.gov/18729326/ DOI: 10.1021/tx800210k
    tissue_or_cell_type
    Urinary mercapturic-acid pathway species

    Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 372–383

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Isotope-dilution LC-MS/MS assay validation in intervention urines · source_derived_draft · unverified_draft

    ### sulforaphane-urine-nac Sulforaphane-N-acetylcysteine was separately quantified in human urine after broccoli sprout exposure. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: This metabolite has its own identity and can be measured separately from the parent. organism: Human, 98 urine specimens tissue_or_cell_type: Urinary mercapturic-acid pathway species experimental_model: Isotope-dilution LC-MS/MS assay validation in intervention urines limitations: Analytical exposure study; metabolite concentration is not tissue efficacy and does not identify each responsible human enzyme. exposure: Broccoli sprout intervention specimens with equivalent administered preparation evidence_span: {"source_cache": "artifacts/sulforaphane-research/18729326.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3", "start_char": 0, "end_char": 2172, "text_sha256": "1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3"} [sulforaphane-p18729326] Quantification of sulforaphane mercapturic acid pathway conjugates in human urine by high-performance liquid chromatography and isotope-dilution tandem mass spectrometry. (2008). https://pubmed.ncbi.nlm.nih.gov/18729326/ DOI: 10.1021/tx800210k
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. Adding mustard powder increased mean urinary SFN-NAC from 9.8 to 44.7 micromol per gram creatinine after cooked broccoli.

    Mustard seed myrosinase preparation, study-specific → Urinary sulforaphane and conjugates, assay specified source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/sulforaphane-research/29806738.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "f6b9a289e09015264ee781be4b765486c80783805e5fa5bdb23361e8740fdc01", "start_char": 0, "end_char": 1324, "text_sha256": "f6b9a289e09015264ee781be4b765486c80783805e5fa5bdb23361e8740fdc01"}
    experimental_model
    Randomized crossover meal study
    exposure
    200 g cooked broccoli with or without 1 g brown mustard powder
    limitations
    Urinary SFN-NAC is an exposure marker; study-specific meal is not a guaranteed product conversion factor.
    nutrient_topic
    Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
    organism
    Human, 12 healthy adults
    plain_language
    Adding an active plant enzyme source increased measured exposure.
    primary_references
    [sulforaphane-p29806738] Supplementation of the Diet by Exogenous Myrosinase via Mustard Seeds to Increase the Bioavailability of Sulforaphane in Healthy Human Subjects after the Consumption of Cooked Broccoli. (2018). https://pubmed.ncbi.nlm.nih.gov/29806738/ DOI: 10.1002/mnfr.201700980
    tissue_or_cell_type
    Urinary SFN-NAC

    Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 983–994

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Randomized crossover meal study · source_derived_draft · unverified_draft

    ### sulforaphane-mustard-food Adding mustard powder increased mean urinary SFN-NAC from 9.8 to 44.7 micromol per gram creatinine after cooked broccoli. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: Adding an active plant enzyme source increased measured exposure. organism: Human, 12 healthy adults tissue_or_cell_type: Urinary SFN-NAC experimental_model: Randomized crossover meal study limitations: Urinary SFN-NAC is an exposure marker; study-specific meal is not a guaranteed product conversion factor. exposure: 200 g cooked broccoli with or without 1 g brown mustard powder evidence_span: {"source_cache": "artifacts/sulforaphane-research/29806738.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "f6b9a289e09015264ee781be4b765486c80783805e5fa5bdb23361e8740fdc01", "start_char": 0, "end_char": 1324, "text_sha256": "f6b9a289e09015264ee781be4b765486c80783805e5fa5bdb23361e8740fdc01"} [sulforaphane-p29806738] Supplementation of the Diet by Exogenous Myrosinase via Mustard Seeds to Increase the Bioavailability of Sulforaphane in Healthy Human Subjects after the Consumption of Cooked Broccoli. (2018). https://pubmed.ncbi.nlm.nih.gov/29806738/ DOI: 10.1002/mnfr.201700980
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards