{"id":"8fbf6761-5382-5985-bbe2-e29f93e2f4e0","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-nac-hdac","predicate":"inhibited","statement":"SFN-NAC also inhibited HDAC activity in the in vitro comparison.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3783ca90-27dd-5c53-9397-b269a4023be2","mechanism_event_label":"Another separately tracked conjugate affected this enzyme readout.","subject":{"id":"b197c092-a606-5689-9549-0b79923a2801","slug":"sulforaphane-n-acetylcysteine","display_name":"Sulforaphane-N-acetylcysteine conjugate / SFN-NAC","entity_type_key":"small_molecule"},"object":{"id":"256770e5-bbe5-55be-8b4b-e697a269d391","slug":"hdac-activity","display_name":"histone deacetylase activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3783ca90-27dd-5c53-9397-b269a4023be2","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-nac-hdac-event","event_type":"biochemical_relationship","label":"Another separately tracked conjugate affected this enzyme readout.","description":"SFN-NAC also inhibited HDAC activity in the in vitro comparison.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b197c092-a606-5689-9549-0b79923a2801","slug":"sulforaphane-n-acetylcysteine","display_name":"Sulforaphane-N-acetylcysteine conjugate / SFN-NAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"256770e5-bbe5-55be-8b4b-e697a269d391","slug":"hdac-activity","display_name":"histone deacetylase activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/15313918.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e\", \"start_char\": 0, \"end_char\": 1861, \"text_sha256\": \"9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell exposure, enzyme assays, metabolites and chromatin immunoprecipitation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Parent SFN, SFN-GSH, SFN-Cys and SFN-NAC; GST inhibition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High-level HDAC activity was measured without establishing universal isoform inhibition; cell effects are not cancer-treatment outcomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human HEK293 and HCT116 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Another separately tracked conjugate affected this enzyme readout.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p15313918] A novel mechanism of chemoprotection by sulforaphane: inhibition of histone deacetylase. (2004). https://pubmed.ncbi.nlm.nih.gov/15313918/ DOI: 10.1158/0008-5472.can-04-1326","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Histone deacetylase activity and histone acetylation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"73981d52-8b42-54c8-a299-336fe40415b4","evidence_kind":"source_excerpt","locator":"Lines 580-591","start_line":580,"end_line":591,"excerpt":"### sulforaphane-nac-hdac\nSFN-NAC also inhibited HDAC activity in the in vitro comparison.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Another separately tracked conjugate affected this enzyme readout.\norganism: Human HEK293 and HCT116 cells\ntissue_or_cell_type: Histone deacetylase activity and histone acetylation\nexperimental_model: Cell exposure, enzyme assays, metabolites and chromatin immunoprecipitation\nlimitations: High-level HDAC activity was measured without establishing universal isoform inhibition; cell effects are not cancer-treatment outcomes.\nexposure: Parent SFN, SFN-GSH, SFN-Cys and SFN-NAC; GST inhibition\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/15313918.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e\", \"start_char\": 0, \"end_char\": 1861, \"text_sha256\": \"9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e\"}\n[sulforaphane-p15313918] A novel mechanism of chemoprotection by sulforaphane: inhibition of histone deacetylase. (2004). https://pubmed.ncbi.nlm.nih.gov/15313918/ DOI: 10.1158/0008-5472.can-04-1326","model_system":"Cell exposure, enzyme assays, metabolites and chromatin immunoprecipitation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sulforaphane-p15313918] A novel mechanism of chemoprotection by sulforaphane: inhibition of histone deacetylase. (2004). https://pubmed.ncbi.nlm.nih.gov/15313918/ DOI: 10.1158/0008-5472.can-04-1326","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"a4bafbf7-f117-53c9-a6d7-8fb25e4aca72","stable_key":"import-44eaeae5-557a-552b-8998-884f30462e2a","title":"Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f355c492a6c35673d8260b1328667fe206e5315b9fcaa5aeff53d80373c7ef97","revision_id":"f2c47d71-34cd-5160-8e92-fe196b576707","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}