Component
Human SLC25A19 G125S variant
Disease-associated mitochondrial transporter carrying Gly125Ser.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
G125S carrier constructs retained mitochondrial targeting despite impaired uptake.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence-scope
- HepG2 cells
- evidence_locator
- Abstract
- evidence_spans
- [{"source_document": "artifacts/thiamine_transport_sources/subramanian-2013-mitochondrial-uptake-source-record.json", "source_field": "resultList.result[0].abstractText", "start_char": 0, "end_char": 1750}]
- experimental_model
- Mouse liver mitochondria and human SLC25A19 constructs in HepG2 cells.
- limitations
- Imaging identifies organellar targeting; carrier is in the inner membrane, not soluble matrix.
- nutrient_topic
- Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
- organism
- Homo sapiens
- plain_language
- Reaching the correct organelle does not guarantee carrier function.
- primary_references
- [subramanian-2013-mitochondrial-uptake] Mitochondrial Uptake of Thiamin Pyrophosphate: Physiological and Cell Biological Aspects (2013). https://pmc.ncbi.nlm.nih.gov/articles/PMC3758298/ DOI: 10.1371/journal.pone.0073503
- spatial_context
- Mitochondrial localization of membrane carrier.
- tissue_or_cell_type
- HepG2 cells
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 473–486
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Mouse liver mitochondria and human SLC25A19 constructs in HepG2 cells. · source_derived_draft · unverified_draft
### b1-slc25a19-g125s-targeting G125S carrier constructs retained mitochondrial targeting despite impaired uptake. Condition category: machinery_impairment nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: Reaching the correct organelle does not guarantee carrier function. organism: Homo sapiens tissue_or_cell_type: HepG2 cells experimental_model: Mouse liver mitochondria and human SLC25A19 constructs in HepG2 cells. limitations: Imaging identifies organellar targeting; carrier is in the inner membrane, not soluble matrix. spatial_context: Mitochondrial localization of membrane carrier. evidence_spans: [{"source_document": "artifacts/thiamine_transport_sources/subramanian-2013-mitochondrial-uptake-source-record.json", "source_field": "resultList.result[0].abstractText", "start_char": 0, "end_char": 1750}] evidence_locator: Abstract evidence-scope: HepG2 cells [subramanian-2013-mitochondrial-uptake] Mitochondrial Uptake of Thiamin Pyrophosphate: Physiological and Cell Biological Aspects (2013). https://pmc.ncbi.nlm.nih.gov/articles/PMC3758298/ DOI: 10.1371/journal.pone.0073503
Complete structured claim and evidenceG125S expression reduced mitochondrial radiolabeled-ThDP uptake and transporter protein abundance relative to wild type.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence-scope
- HepG2 mitochondria
- evidence_locator
- Abstract
- evidence_spans
- [{"source_document": "artifacts/thiamine_transport_sources/subramanian-2013-mitochondrial-uptake-source-record.json", "source_field": "resultList.result[0].abstractText", "start_char": 0, "end_char": 1750}]
- experimental_model
- Mouse liver mitochondria and human SLC25A19 constructs in HepG2 cells.
- limitations
- Lower abundance prevents assigning the entire effect to transport kinetics.
- nutrient_topic
- Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
- organism
- Homo sapiens
- plain_language
- This inherited carrier variant limits mitochondrial cofactor uptake.
- primary_references
- [subramanian-2013-mitochondrial-uptake] Mitochondrial Uptake of Thiamin Pyrophosphate: Physiological and Cell Biological Aspects (2013). https://pmc.ncbi.nlm.nih.gov/articles/PMC3758298/ DOI: 10.1371/journal.pone.0073503
- tissue_or_cell_type
- HepG2 mitochondria
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 459–471
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Mouse liver mitochondria and human SLC25A19 constructs in HepG2 cells. · source_derived_draft · unverified_draft
### b1-slc25a19-g125s-uptake G125S expression reduced mitochondrial radiolabeled-ThDP uptake and transporter protein abundance relative to wild type. Condition category: machinery_impairment nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: This inherited carrier variant limits mitochondrial cofactor uptake. organism: Homo sapiens tissue_or_cell_type: HepG2 mitochondria experimental_model: Mouse liver mitochondria and human SLC25A19 constructs in HepG2 cells. limitations: Lower abundance prevents assigning the entire effect to transport kinetics. evidence_spans: [{"source_document": "artifacts/thiamine_transport_sources/subramanian-2013-mitochondrial-uptake-source-record.json", "source_field": "resultList.result[0].abstractText", "start_char": 0, "end_char": 1750}] evidence_locator: Abstract evidence-scope: HepG2 mitochondria [subramanian-2013-mitochondrial-uptake] Mitochondrial Uptake of Thiamin Pyrophosphate: Physiological and Cell Biological Aspects (2013). https://pmc.ncbi.nlm.nih.gov/articles/PMC3758298/ DOI: 10.1371/journal.pone.0073503
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.