{"id":"c284171c-6a9d-544b-8991-12fd09d6dc11","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-slc25a19-g125s-targeting","predicate":"retains-targeting-to","statement":"G125S carrier constructs retained mitochondrial targeting despite impaired uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"1d3e6997-efed-5390-8266-e740280a3b0e","mechanism_event_label":"Reaching the correct organelle does not guarantee carrier function.","subject":{"id":"fce52ac1-1d99-5600-8dd7-9d1c8bfe9ec1","slug":"slc25a19-g125s","display_name":"Human SLC25A19 G125S variant","entity_type_key":"protein_state"},"object":{"id":"f5bd4edd-4298-5985-976f-897fd415c3d5","slug":"mitochondria","display_name":"Mitochondria","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"1d3e6997-efed-5390-8266-e740280a3b0e","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-slc25a19-g125s-targeting-event","event_type":"biochemical_relationship","label":"Reaching the correct organelle does not guarantee carrier function.","description":"G125S carrier constructs retained mitochondrial targeting despite impaired uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3f719fb0-6cf0-574a-a56f-3c43dfcb681a","slug":"slc25a19","display_name":"Human mitochondrial thiamine diphosphate transporter / SLC25A19","entity_type_key":"protein"},"role":"wild-type comparator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fce52ac1-1d99-5600-8dd7-9d1c8bfe9ec1","slug":"slc25a19-g125s","display_name":"Human SLC25A19 G125S variant","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f5bd4edd-4298-5985-976f-897fd415c3d5","slug":"mitochondria","display_name":"Mitochondria","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence-scope","value_text":"HepG2 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_locator","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_spans","value_text":"[{\"source_document\": \"artifacts/thiamine_transport_sources/subramanian-2013-mitochondrial-uptake-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1750}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse liver mitochondria and human SLC25A19 constructs in HepG2 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Imaging identifies organellar targeting; carrier is in the inner membrane, not soluble matrix.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Reaching the correct organelle does not guarantee carrier function.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[subramanian-2013-mitochondrial-uptake] Mitochondrial Uptake of Thiamin Pyrophosphate: Physiological and Cell Biological Aspects (2013). https://pmc.ncbi.nlm.nih.gov/articles/PMC3758298/ DOI: 10.1371/journal.pone.0073503","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"spatial_context","value_text":"Mitochondrial localization of membrane carrier.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HepG2 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"cc424d46-cfe6-521b-9a47-b0365dade457","evidence_kind":"source_excerpt","locator":"Lines 473-486","start_line":473,"end_line":486,"excerpt":"### b1-slc25a19-g125s-targeting\nG125S carrier constructs retained mitochondrial targeting despite impaired uptake.\nCondition category: machinery_impairment\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Reaching the correct organelle does not guarantee carrier function.\norganism: Homo sapiens\ntissue_or_cell_type: HepG2 cells\nexperimental_model: Mouse liver mitochondria and human SLC25A19 constructs in HepG2 cells.\nlimitations: Imaging identifies organellar targeting; carrier is in the inner membrane, not soluble matrix.\nspatial_context: Mitochondrial localization of membrane carrier.\nevidence_spans: [{\"source_document\": \"artifacts/thiamine_transport_sources/subramanian-2013-mitochondrial-uptake-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1750}]\nevidence_locator: Abstract\nevidence-scope: HepG2 cells\n[subramanian-2013-mitochondrial-uptake] Mitochondrial Uptake of Thiamin Pyrophosphate: Physiological and Cell Biological Aspects (2013). https://pmc.ncbi.nlm.nih.gov/articles/PMC3758298/ DOI: 10.1371/journal.pone.0073503","model_system":"Mouse liver mitochondria and human SLC25A19 constructs in HepG2 cells.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [subramanian-2013-mitochondrial-uptake] Mitochondrial Uptake of Thiamin Pyrophosphate: Physiological and Cell Biological Aspects (2013). https://pmc.ncbi.nlm.nih.gov/articles/PMC3758298/ DOI: 10.1371/journal.pone.0073503","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}