Component

Renal proximal-tubule basolateral calcium/magnesium ATPase assay preparation

Context-specific entity; species, compartment and exposure are stated on each claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. The reported uptake half-saturation concentrations were about 13.4 micromolar for Sr2+ and 0.28 micromolar for Ca2+, consistent with lower affinity for strontium.

    Renal proximal-tubule basolateral calcium/magnesium ATPase assay preparation → Strontium ion / Sr2+ source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text
    experimental_model
    Renal-vesicle uptake kinetics.
    limitations
    Assay-specific apparent affinities; do not assign them to every calcium channel or human compartment.
    nutrient_topic
    Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Strontium
    plain_language
    Using the same pump does not mean being transported equally efficiently.
    primary_references
    A Comparison Between Calcium and Strontium Transport by the (Ca2+ + Mg2+)ATPase of the Basolateral Plasma Membrane of Renal Proximal Convoluted Tubules. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40277992/ · DOI 10.3390/membranes15040122

    Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19) · lines 30–36

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Renal-vesicle uptake kinetics. · source_derived_draft · unverified_draft

    ## strontium-renal-affinity Using the same pump does not mean being transported equally efficiently. The reported uptake half-saturation concentrations were about 13.4 micromolar for Sr2+ and 0.28 micromolar for Ca2+, consistent with lower affinity for strontium. Model: Renal-vesicle uptake kinetics. Limitations: Assay-specific apparent affinities; do not assign them to every calcium channel or human compartment. Evidence access: Primary full text A Comparison Between Calcium and Strontium Transport by the (Ca2+ + Mg2+)ATPase of the Basolateral Plasma Membrane of Renal Proximal Convoluted Tubules. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40277992/ · DOI 10.3390/membranes15040122
    Complete structured claim and evidence

  2. Basolateral proximal-tubule membrane vesicles accumulated radiolabeled strontium in an ATP- and Mg2+-dependent transport assay.

    Renal proximal-tubule basolateral calcium/magnesium ATPase assay preparation → Strontium ion / Sr2+ source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text
    experimental_model
    Isolated renal membrane vesicles; 90Sr and 45Ca tracers; ATP/Mg uptake assays.
    limitations
    Accessed methods refer to a prior preparation protocol without resolving species or ATP2B isoform. The assay measures ion transport, not radiation injury or net human renal clearance.
    nutrient_topic
    Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Strontium
    plain_language
    A calcium-handling membrane preparation can also pump strontium.
    primary_references
    A Comparison Between Calcium and Strontium Transport by the (Ca2+ + Mg2+)ATPase of the Basolateral Plasma Membrane of Renal Proximal Convoluted Tubules. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40277992/ · DOI 10.3390/membranes15040122
    transport_effect
    depends Membrane vesicles accumulated strontium in the assay, while the basolateral pump's role in the intact cell is extrusion toward blood; the two pools move opposite ways.
    transport_pool
    the proximal-tubule cell interior Membrane vesicles accumulated strontium in the assay, while the basolateral pump's role in the intact cell is extrusion toward blood; the two pools move opposite ways.

    Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19) · lines 14–20

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Isolated renal membrane vesicles; 90Sr and 45Ca tracers; ATP/Mg uptake assays. · source_derived_draft · unverified_draft

    ## strontium-renal-uptake A calcium-handling membrane preparation can also pump strontium. Basolateral proximal-tubule membrane vesicles accumulated radiolabeled strontium in an ATP- and Mg2+-dependent transport assay. Model: Isolated renal membrane vesicles; 90Sr and 45Ca tracers; ATP/Mg uptake assays. Limitations: Accessed methods refer to a prior preparation protocol without resolving species or ATP2B isoform. The assay measures ion transport, not radiation injury or net human renal clearance. Evidence access: Primary full text A Comparison Between Calcium and Strontium Transport by the (Ca2+ + Mg2+)ATPase of the Basolateral Plasma Membrane of Renal Proximal Convoluted Tubules. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40277992/ · DOI 10.3390/membranes15040122
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. Omission of ATP or magnesium prevented strontium uptake in the tested renal membrane preparation; other tested nucleotides did not substitute for ATP.

    Mg2+ → Strontium uptake by renal membrane vesicles source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_access
    Primary full text
    experimental_model
    Isolated membrane-vesicle cofactor-dependence experiments.
    limitations
    Cofactor removal is experimental machinery impairment, not proof that dietary magnesium deficiency changes human strontium retention by a specified amount.
    nutrient_topic
    Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Strontium
    plain_language
    The pump needs both its energy source and magnesium.
    primary_references
    A Comparison Between Calcium and Strontium Transport by the (Ca2+ + Mg2+)ATPase of the Basolateral Plasma Membrane of Renal Proximal Convoluted Tubules. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40277992/ · DOI 10.3390/membranes15040122
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19) · lines 38–44

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Isolated membrane-vesicle cofactor-dependence experiments. · source_derived_draft · unverified_draft

    ## strontium-renal-cofactor-loss The pump needs both its energy source and magnesium. Omission of ATP or magnesium prevented strontium uptake in the tested renal membrane preparation; other tested nucleotides did not substitute for ATP. Model: Isolated membrane-vesicle cofactor-dependence experiments. Limitations: Cofactor removal is experimental machinery impairment, not proof that dietary magnesium deficiency changes human strontium retention by a specified amount. Evidence access: Primary full text A Comparison Between Calcium and Strontium Transport by the (Ca2+ + Mg2+)ATPase of the Basolateral Plasma Membrane of Renal Proximal Convoluted Tubules. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40277992/ · DOI 10.3390/membranes15040122
    Complete structured claim and evidence

  2. Calcium and strontium each competitively inhibited uptake of the other in the renal-vesicle assay, increasing apparent half-saturation concentration without changing maximum velocity.

    Strontium ion / Sr2+ → Calcium uptake by renal basolateral membrane vesicles source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text
    experimental_model
    Radiotracer uptake in isolated renal basolateral membranes.
    limitations
    Shared-site kinetics do not establish a dietary calcium-loss threshold or quantify whole-kidney excretion.
    nutrient_topic
    Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Strontium
    plain_language
    The two ions compete for access to the same transport machinery.
    primary_references
    A Comparison Between Calcium and Strontium Transport by the (Ca2+ + Mg2+)ATPase of the Basolateral Plasma Membrane of Renal Proximal Convoluted Tubules. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40277992/ · DOI 10.3390/membranes15040122

    Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19) · lines 22–28

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Radiotracer uptake in isolated renal basolateral membranes. · source_derived_draft · unverified_draft

    ## strontium-renal-competition The two ions compete for access to the same transport machinery. Calcium and strontium each competitively inhibited uptake of the other in the renal-vesicle assay, increasing apparent half-saturation concentration without changing maximum velocity. Model: Radiotracer uptake in isolated renal basolateral membranes. Limitations: Shared-site kinetics do not establish a dietary calcium-loss threshold or quantify whole-kidney excretion. Evidence access: Primary full text A Comparison Between Calcium and Strontium Transport by the (Ca2+ + Mg2+)ATPase of the Basolateral Plasma Membrane of Renal Proximal Convoluted Tubules. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40277992/ · DOI 10.3390/membranes15040122
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards