{"id":"0b2e026b-4403-5403-a8b8-0653996dc886","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-renal-uptake","predicate":"transports","statement":"Basolateral proximal-tubule membrane vesicles accumulated radiolabeled strontium in an ATP- and Mg2+-dependent transport assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f67a861c-09b0-533f-a972-7abc1ab92526","mechanism_event_label":"A calcium-handling membrane preparation can also pump strontium.","subject":{"id":"d46a8d5b-1a2d-5e93-8b06-a005ee199285","slug":"renal-basolateral-calcium-magnesium-atpase-assay","display_name":"Renal proximal-tubule basolateral calcium/magnesium ATPase assay preparation","entity_type_key":"protein_set"},"object":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"f67a861c-09b0-533f-a972-7abc1ab92526","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-renal-uptake-event","event_type":"observed_relationship","label":"A calcium-handling membrane preparation can also pump strontium.","description":"Basolateral proximal-tubule membrane vesicles accumulated radiolabeled strontium in an ATP- and Mg2+-dependent transport assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d46a8d5b-1a2d-5e93-8b06-a005ee199285","slug":"renal-basolateral-calcium-magnesium-atpase-assay","display_name":"Renal proximal-tubule basolateral calcium/magnesium ATPase assay preparation","entity_type_key":"protein_set"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2ca2864b-1ef0-54e4-8397-1dbe7207a0d2","slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated renal membrane vesicles; 90Sr and 45Ca tracers; ATP/Mg uptake assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Accessed methods refer to a prior preparation protocol without resolving species or ATP2B isoform. The assay measures ion transport, not radiation injury or net human renal clearance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"A calcium-handling membrane preparation can also pump strontium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A Comparison Between Calcium and Strontium Transport by the (Ca2+ + Mg2+)ATPase of the Basolateral Plasma Membrane of Renal Proximal Convoluted Tubules. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40277992/ · DOI 10.3390/membranes15040122","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"depends","comparator":null,"unit":null,"notes":"Membrane vesicles accumulated strontium in the assay, while the basolateral pump's role in the intact cell is extrusion toward blood; the two pools move opposite ways.","entity":null},{"dimension":"transport_pool","value_text":"the proximal-tubule cell interior","comparator":null,"unit":null,"notes":"Membrane vesicles accumulated strontium in the assay, while the basolateral pump's role in the intact cell is extrusion toward blood; the two pools move opposite ways.","entity":null}],"evidence":[{"id":"d398fcab-6007-579a-95c1-683820022fdb","evidence_kind":"source_excerpt","locator":"Lines 14-20","start_line":14,"end_line":20,"excerpt":"## strontium-renal-uptake\nA calcium-handling membrane preparation can also pump strontium.\nBasolateral proximal-tubule membrane vesicles accumulated radiolabeled strontium in an ATP- and Mg2+-dependent transport assay.\nModel: Isolated renal membrane vesicles; 90Sr and 45Ca tracers; ATP/Mg uptake assays.\nLimitations: Accessed methods refer to a prior preparation protocol without resolving species or ATP2B isoform. The assay measures ion transport, not radiation injury or net human renal clearance.\nEvidence access: Primary full text\nA Comparison Between Calcium and Strontium Transport by the (Ca2+ + Mg2+)ATPase of the Basolateral Plasma Membrane of Renal Proximal Convoluted Tubules. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40277992/ · DOI 10.3390/membranes15040122","model_system":"Isolated renal membrane vesicles; 90Sr and 45Ca tracers; ATP/Mg uptake assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"43ef0fe7-eb6c-5346-8381-f7694733b4dc","stable_key":"import-1ff3a29f-ef80-5bff-9300-30b98c60b90d","title":"Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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