Component
Rat dihydrolipoamide S-acetyltransferase Dlat
Rat dihydrolipoamide S-acetyltransferase Dlat. Species, exposure and limitations are retained in each linked claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Dlat and HADHA showed proximity and colocalization in neonatal rat cardiomyocytes.
Experimental context and source evidence
- access_level
- full_text_and_supplement_review
- compartment
- Mitochondria or cell lysate, depending on assay
- dose
- No treatment dose assigned to this localization observation
- duration
- Not established in reviewed text
- endpoint
- rat-hadha
- evidence_location
- Proximity ligation and immunofluorescence; Figure 6F/G
- experimental_model
- Protein proximity/colocalization in NRVCMs
- exposure
- rat-dlat
- limitations
- Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Proximity is not proof of a direct physical contact or transfer reaction.
- organism
- Rattus norvegicus host cells; construct species may be unspecified
- plain_language
- Dlat and HADHA showed proximity and colocalization in neonatal rat cardiomyocytes.
- primary_locator
- [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 44, "text_sha256": "e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621", "xml_element_id": "Par19"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 46, "text_sha256": "d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff", "xml_element_id": null}]
- primary_references
- https://doi.org/10.1038/s41467-026-70703-w
- sample_size
- 4 biological replicates
- tissue_or_cell_type
- Neonatal rat ventricular cardiomyocytes (NRVCMs)
DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 34–49
AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Protein proximity/colocalization in NRVCMs · source_derived_draft · unverified_draft
Dlat and HADHA showed proximity and colocalization in neonatal rat cardiomyocytes. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Protein proximity/colocalization in NRVCMs compartment: Mitochondria or cell lysate, depending on assay dose: No treatment dose assigned to this localization observation duration: Not established in reviewed text primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Proximity ligation and immunofluorescence; Figure 6F/G endpoint: rat-hadha exposure: rat-dlat limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Proximity is not proof of a direct physical contact or transfer reaction. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 44, "text_sha256": "e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621", "xml_element_id": "Par19"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 46, "text_sha256": "d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff", "xml_element_id": null}] plain_language: Dlat and HADHA showed proximity and colocalization in neonatal rat cardiomyocytes. sample_size: 4 biological replicates
Complete structured claim and evidence
Where it participates (unsigned role)
Slc25a3-null rat cells exposed to elesclomol-copper showed increased DLAT oligomerization and loss of lipoylated DLAT and DLST.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_span
- {"source_cache": "artifacts/copper-research/42308035.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "0c19d3c109f1df88791ad51c22e6acf85b7d5ffe5c26c74aaaf5e3222a75c054", "start_char": 5507, "end_char": 5866, "text_sha256": "77f0d121a0aed5bdcd3c4898c369533a29208ebd8a9450a3a37ceff5d783c3af"}
- experimental_model
- Rat Slc25a3 knockout cardiomyoblasts and human transporter expression in bacteria
- exposure
- Slc25a3 deletion and elesclomol-copper exposure
- limitations
- Whole-organelle copper was measured, not separate matrix and intermembrane pools. Matrix trapping is the authors mechanism inferred with transport evidence. This recent study complements import findings; bacterial export does not reproduce mitochondrial topology.
- nutrient_topic
- Copper research collection; topical membership is not evidence of a direct dietary effect. · Copper
- organism
- Rat H9c2 cells; human SLC25A3 in Lactococcus lactis
- plain_language
- Misplaced copper damaged proteins that normally carry lipoamide.
- primary_references
- [copper-p42308035] SLC25A3 exports mitochondrial copper to metalate cytochrome c oxidase and prevent cuproptosis. (2026). https://pubmed.ncbi.nlm.nih.gov/42308035/ DOI: 10.1073/pnas.2612098123
- tissue_or_cell_type
- Mitochondria; bacterial copper export assay
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Copper: transport, cuproenzymes, deficiency, excess and nutrient interactions (2026-09-17) · lines 572–583
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat Slc25a3 knockout cardiomyoblasts and human transporter expression in bacteria · source_derived_draft · unverified_draft
### copper-slc25a3-es-dlat Slc25a3-null rat cells exposed to elesclomol-copper showed increased DLAT oligomerization and loss of lipoylated DLAT and DLST. Condition category: machinery_impairment nutrient_topic: Copper research collection; topical membership is not evidence of a direct dietary effect. plain_language: Misplaced copper damaged proteins that normally carry lipoamide. organism: Rat H9c2 cells; human SLC25A3 in Lactococcus lactis tissue_or_cell_type: Mitochondria; bacterial copper export assay experimental_model: Rat Slc25a3 knockout cardiomyoblasts and human transporter expression in bacteria limitations: Whole-organelle copper was measured, not separate matrix and intermembrane pools. Matrix trapping is the authors mechanism inferred with transport evidence. This recent study complements import findings; bacterial export does not reproduce mitochondrial topology. exposure: Slc25a3 deletion and elesclomol-copper exposure evidence_span: {"source_cache": "artifacts/copper-research/42308035.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "0c19d3c109f1df88791ad51c22e6acf85b7d5ffe5c26c74aaaf5e3222a75c054", "start_char": 5507, "end_char": 5866, "text_sha256": "77f0d121a0aed5bdcd3c4898c369533a29208ebd8a9450a3a37ceff5d783c3af"} [copper-p42308035] SLC25A3 exports mitochondrial copper to metalate cytochrome c oxidase and prevent cuproptosis. (2026). https://pubmed.ncbi.nlm.nih.gov/42308035/ DOI: 10.1073/pnas.2612098123
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.