{"id":"40e63301-dbf7-5a10-9126-c47a29afbaa6","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:rat-dlat-hadha-proximity","predicate":"is_proximal_to","statement":"Dlat and HADHA showed proximity and colocalization in neonatal rat cardiomyocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4f04c198-ed2c-5a9c-b0c6-c0ecc0758d1f","mechanism_event_label":"rat dlat hadha proximity","subject":{"id":"3c9a688d-6143-5a41-a63d-385ac8037ab0","slug":"rat-dlat","display_name":"Rat dihydrolipoamide S-acetyltransferase Dlat","entity_type_key":"protein"},"object":{"id":"3acd8a9d-0b7e-5892-b35f-5ecbd005c352","slug":"rat-hadha","display_name":"Rat mitochondrial trifunctional protein alpha subunit / Hadha","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4f04c198-ed2c-5a9c-b0c6-c0ecc0758d1f","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:rat-dlat-hadha-proximity","event_type":"is_proximal_to","label":"rat dlat hadha proximity","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3c9a688d-6143-5a41-a63d-385ac8037ab0","slug":"rat-dlat","display_name":"Rat dihydrolipoamide S-acetyltransferase Dlat","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3acd8a9d-0b7e-5892-b35f-5ecbd005c352","slug":"rat-hadha","display_name":"Rat mitochondrial trifunctional protein alpha subunit / Hadha","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Mitochondria or cell lysate, depending on assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"No treatment dose assigned to this localization observation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not established in reviewed text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"rat-hadha","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Proximity ligation and immunofluorescence; Figure 6F/G","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Protein proximity/colocalization in NRVCMs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"rat-dlat","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Proximity is not proof of a direct physical contact or transfer reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rattus norvegicus host cells; construct species may be unspecified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Dlat and HADHA showed proximity and colocalization in neonatal rat cardiomyocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 44, \"text_sha256\": \"e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621\", \"xml_element_id\": \"Par19\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 46, \"text_sha256\": \"d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff\", \"xml_element_id\": null}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"4 biological replicates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neonatal rat ventricular cardiomyocytes (NRVCMs)","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"30b59a64-918b-59a2-a02c-ea68d1c92e26","evidence_kind":"source_excerpt","locator":"Lines 34-49","start_line":34,"end_line":49,"excerpt":"Dlat and HADHA showed proximity and colocalization in neonatal rat cardiomyocytes.\norganism: Rattus norvegicus host cells; construct species may be unspecified\ntissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs)\nexperimental_model: Protein proximity/colocalization in NRVCMs\ncompartment: Mitochondria or cell lysate, depending on assay\ndose: No treatment dose assigned to this localization observation\nduration: Not established in reviewed text\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Proximity ligation and immunofluorescence; Figure 6F/G\nendpoint: rat-hadha\nexposure: rat-dlat\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Proximity is not proof of a direct physical contact or transfer reaction.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 44, \"text_sha256\": \"e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621\", \"xml_element_id\": \"Par19\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 46, \"text_sha256\": \"d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff\", \"xml_element_id\": null}]\nplain_language: Dlat and HADHA showed proximity and colocalization in neonatal rat cardiomyocytes.\nsample_size: 4 biological replicates","model_system":"Protein proximity/colocalization in NRVCMs","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}