Component
Propionate-derived carbon incorporation into cellular protein
Propionate-derived carbon incorporation into cellular protein
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
MMUT knockout reduced propionate-derived protein labeling about sixfold in HFF1 human fibroblasts.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- evidence_location
- Full text Results; Figure 2C,E
- experimental_model
- HFF1 human fibroblasts with two CRISPR guides
- exposure
- MMUT CRISPR knockout versus OR7G3 guide control; [14C]propionate
- limitations
- Protein labeling integrates metabolism and protein synthesis; not direct MMUT flux.
- nutrient_topic
- Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B12 (cobalamins)
- organism
- Homo sapiens
- plain_language
- These human fibroblasts relied strongly on MMUT for the measured use of propionate carbon.
- primary_references
- [2024-propionate-cell-dependence] Differential utilization of vitamin B12-dependent and independent pathways for propionate metabolism across human cells. (2024). https://pubmed.ncbi.nlm.nih.gov/39128713/ DOI: 10.1016/j.jbc.2024.107662
- tissue_or_cell_type
- Foreskin fibroblasts
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1300–1312
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · HFF1 human fibroblasts with two CRISPR guides · source_derived_draft · unverified_draft
### hff1-mmut-ko-lowers-propionate-label MMUT knockout reduced propionate-derived protein labeling about sixfold in HFF1 human fibroblasts. Condition category: machinery_impairment nutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. plain_language: These human fibroblasts relied strongly on MMUT for the measured use of propionate carbon. organism: Homo sapiens tissue_or_cell_type: Foreskin fibroblasts experimental_model: HFF1 human fibroblasts with two CRISPR guides limitations: Protein labeling integrates metabolism and protein synthesis; not direct MMUT flux. exposure: MMUT CRISPR knockout versus OR7G3 guide control; [14C]propionate cross_nutrient: false evidence_location: Full text Results; Figure 2C,E [2024-propionate-cell-dependence] Differential utilization of vitamin B12-dependent and independent pathways for propionate metabolism across human cells. (2024). https://pubmed.ncbi.nlm.nih.gov/39128713/ DOI: 10.1016/j.jbc.2024.107662
Complete structured claim and evidenceMMUT knockout did not reduce propionate-derived protein labeling in HT-29 colorectal cancer cells.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- evidence_location
- Full text Results; Figure 2C,E
- experimental_model
- HT-29 human colorectal adenocarcinoma cells
- exposure
- MMUT CRISPR knockout versus OR7G3 guide control; [14C]propionate
- limitations
- Transformed cell line; does not establish pathway proportions in normal human colon.
- nutrient_topic
- Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B12 (cobalamins)
- organism
- Homo sapiens
- plain_language
- This human cancer cell line retained propionate use without MMUT, unlike the tested fibroblasts.
- primary_references
- [2024-propionate-cell-dependence] Differential utilization of vitamin B12-dependent and independent pathways for propionate metabolism across human cells. (2024). https://pubmed.ncbi.nlm.nih.gov/39128713/ DOI: 10.1016/j.jbc.2024.107662
- tissue_or_cell_type
- Colorectal adenocarcinoma cells
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1314–1326
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · HT-29 human colorectal adenocarcinoma cells · source_derived_draft · unverified_draft
### ht29-mmut-ko-preserves-propionate-label MMUT knockout did not reduce propionate-derived protein labeling in HT-29 colorectal cancer cells. Condition category: machinery_impairment nutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. plain_language: This human cancer cell line retained propionate use without MMUT, unlike the tested fibroblasts. organism: Homo sapiens tissue_or_cell_type: Colorectal adenocarcinoma cells experimental_model: HT-29 human colorectal adenocarcinoma cells limitations: Transformed cell line; does not establish pathway proportions in normal human colon. exposure: MMUT CRISPR knockout versus OR7G3 guide control; [14C]propionate cross_nutrient: false evidence_location: Full text Results; Figure 2C,E [2024-propionate-cell-dependence] Differential utilization of vitamin B12-dependent and independent pathways for propionate metabolism across human cells. (2024). https://pubmed.ncbi.nlm.nih.gov/39128713/ DOI: 10.1016/j.jbc.2024.107662
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.