{"id":"c93e17ba-b590-5510-be74-4dab5ef116ef","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:ht29-mmut-ko-preserves-propionate-label","predicate":"knockout-does-not-reduce","statement":"MMUT knockout did not reduce propionate-derived protein labeling in HT-29 colorectal cancer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"ada82c74-b751-5e29-aff0-5e1c6af7fbf0","mechanism_event_label":"This human cancer cell line retained propionate use without MMUT, unlike the tested fibroblasts.","subject":{"id":"7c1e45c3-bb73-5e63-8b3d-4bb3c96ed819","slug":"mmut-human-gene","display_name":"MMUT gene (Homo sapiens)","entity_type_key":"gene"},"object":{"id":"83e7171b-2769-5d0a-b65c-95f5c6bd3b3d","slug":"propionate-carbon-protein-incorporation","display_name":"Propionate-derived carbon incorporation into cellular protein","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ada82c74-b751-5e29-aff0-5e1c6af7fbf0","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:ht29-mmut-ko-preserves-propionate-label-event","event_type":"biochemical_relationship","label":"This human cancer cell line retained propionate use without MMUT, unlike the tested fibroblasts.","description":"MMUT knockout did not reduce propionate-derived protein labeling in HT-29 colorectal cancer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7c1e45c3-bb73-5e63-8b3d-4bb3c96ed819","slug":"mmut-human-gene","display_name":"MMUT gene (Homo sapiens)","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"83e7171b-2769-5d0a-b65c-95f5c6bd3b3d","slug":"propionate-carbon-protein-incorporation","display_name":"Propionate-derived carbon incorporation into cellular protein","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text Results; Figure 2C,E","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HT-29 human colorectal adenocarcinoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"MMUT CRISPR knockout versus OR7G3 guide control; [14C]propionate","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transformed cell line; does not establish pathway proportions in normal human colon.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This human cancer cell line retained propionate use without MMUT, unlike the tested fibroblasts.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[2024-propionate-cell-dependence] Differential utilization of vitamin B12-dependent and independent pathways for propionate metabolism across human cells. (2024). https://pubmed.ncbi.nlm.nih.gov/39128713/ DOI: 10.1016/j.jbc.2024.107662","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Colorectal adenocarcinoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"5f90555e-9a72-5ff9-ad4e-08dcb42fbf94","evidence_kind":"source_excerpt","locator":"Lines 1314-1326","start_line":1314,"end_line":1326,"excerpt":"### ht29-mmut-ko-preserves-propionate-label\nMMUT knockout did not reduce propionate-derived protein labeling in HT-29 colorectal cancer cells.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This human cancer cell line retained propionate use without MMUT, unlike the tested fibroblasts.\norganism: Homo sapiens\ntissue_or_cell_type: Colorectal adenocarcinoma cells\nexperimental_model: HT-29 human colorectal adenocarcinoma cells\nlimitations: Transformed cell line; does not establish pathway proportions in normal human colon.\nexposure: MMUT CRISPR knockout versus OR7G3 guide control; [14C]propionate\ncross_nutrient: false\nevidence_location: Full text Results; Figure 2C,E\n[2024-propionate-cell-dependence] Differential utilization of vitamin B12-dependent and independent pathways for propionate metabolism across human cells. 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