Component
Human NADPH-dependent diflavin oxidoreductase 1
Human NADPH-dependent diflavin oxidoreductase 1
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Recombinant human NDOR1/NR1 with NADPH supported MMACHC-bound cyanocobalamin conversion to cob(II)alamin; free cyanocobalamin was not detectably reduced without MMACHC.
Experimental context and source evidence
- cross_nutrient
- true
- evidence_location
- Results: Figure 4; Materials and Methods: Decyanation of CNCbl
- experimental_model
- Human purified-protein anaerobic reconstitution
- exposure
- 4 micromolar reductase, 20 micromolar MMACHC/CNCbl, 200 micromolar NADPH
- limitations
- Reconstituted biochemical system; establishes reaction capability rather than the quantitatively dominant pathway in living humans.
- nutrient_topic
- Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B12 (cobalamins)
- organism
- Homo sapiens
- plain_language
- This flavoprotein can pass reducing power into B12 processing.
- primary_references
- [kim-2008-decyanation] Decyanation of vitamin B12 by a trafficking chaperone (2008). https://pubmed.ncbi.nlm.nih.gov/18779575/ DOI: 10.1073/pnas.0805989105
- tissue_or_cell_type
- Cell-free assay
Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 697–709
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human purified-protein anaerobic reconstitution · source_derived_draft · unverified_draft
### b12-ndor1-mmachc-electrons Recombinant human NDOR1/NR1 with NADPH supported MMACHC-bound cyanocobalamin conversion to cob(II)alamin; free cyanocobalamin was not detectably reduced without MMACHC. Condition category: normal nutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. plain_language: This flavoprotein can pass reducing power into B12 processing. organism: Homo sapiens tissue_or_cell_type: Cell-free assay experimental_model: Human purified-protein anaerobic reconstitution limitations: Reconstituted biochemical system; establishes reaction capability rather than the quantitatively dominant pathway in living humans. exposure: 4 micromolar reductase, 20 micromolar MMACHC/CNCbl, 200 micromolar NADPH cross_nutrient: true evidence_location: Results: Figure 4; Materials and Methods: Decyanation of CNCbl [kim-2008-decyanation] Decyanation of vitamin B12 by a trafficking chaperone (2008). https://pubmed.ncbi.nlm.nih.gov/18779575/ DOI: 10.1073/pnas.0805989105
Complete structured claim and evidence
Where it participates (unsigned role)
Human MMACHC converted bound cyanocobalamin to cob(II)alamin during NADPH-supported reductive decyanation; EPR identified the product in a base-off coordination state.
Experimental context and source evidence
- cross_nutrient
- true
- evidence_location
- Results: Figure 4; Materials and Methods: Decyanation of CNCbl
- experimental_model
- Purified human MMACHC with human flavoprotein donor
- exposure
- Anaerobic CNCbl/MMACHC, NADPH and MTRR or NDOR1
- limitations
- Reconstituted biochemical system; establishes reaction capability rather than the quantitatively dominant pathway in living humans.
- nutrient_topic
- Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B12 (cobalamins)
- organism
- Homo sapiens
- plain_language
- Processing removes the cyano group and leaves a reduced B12 intermediate.
- primary_references
- [kim-2008-decyanation] Decyanation of vitamin B12 by a trafficking chaperone (2008). https://pubmed.ncbi.nlm.nih.gov/18779575/ DOI: 10.1073/pnas.0805989105
- tissue_or_cell_type
- Cell-free assay
Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 669–681
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purified human MMACHC with human flavoprotein donor · source_derived_draft · unverified_draft
### b12-mmachc-decyanation Human MMACHC converted bound cyanocobalamin to cob(II)alamin during NADPH-supported reductive decyanation; EPR identified the product in a base-off coordination state. Condition category: normal nutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. plain_language: Processing removes the cyano group and leaves a reduced B12 intermediate. organism: Homo sapiens tissue_or_cell_type: Cell-free assay experimental_model: Purified human MMACHC with human flavoprotein donor limitations: Reconstituted biochemical system; establishes reaction capability rather than the quantitatively dominant pathway in living humans. exposure: Anaerobic CNCbl/MMACHC, NADPH and MTRR or NDOR1 cross_nutrient: true evidence_location: Results: Figure 4; Materials and Methods: Decyanation of CNCbl [kim-2008-decyanation] Decyanation of vitamin B12 by a trafficking chaperone (2008). https://pubmed.ncbi.nlm.nih.gov/18779575/ DOI: 10.1073/pnas.0805989105
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.