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Materials and Methods: Decyanation of CNCbl","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human purified-protein anaerobic reconstitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"4 micromolar reductase, 20 micromolar MMACHC/CNCbl, 200 micromolar NADPH","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reconstituted biochemical system; establishes reaction capability rather than the quantitatively dominant pathway in living humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This flavoprotein can pass reducing power into B12 processing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[kim-2008-decyanation] Decyanation of vitamin B12 by a trafficking chaperone (2008). https://pubmed.ncbi.nlm.nih.gov/18779575/ DOI: 10.1073/pnas.0805989105","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ebf39db0-42a2-5207-a8f4-22eff1f0a696","evidence_kind":"source_excerpt","locator":"Lines 697-709","start_line":697,"end_line":709,"excerpt":"### b12-ndor1-mmachc-electrons\nRecombinant human NDOR1/NR1 with NADPH supported MMACHC-bound cyanocobalamin conversion to cob(II)alamin; free cyanocobalamin was not detectably reduced without MMACHC.\nCondition category: normal\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This flavoprotein can pass reducing power into B12 processing.\norganism: Homo sapiens\ntissue_or_cell_type: Cell-free assay\nexperimental_model: Human purified-protein anaerobic reconstitution\nlimitations: Reconstituted biochemical system; establishes reaction capability rather than the quantitatively dominant pathway in living humans.\nexposure: 4 micromolar reductase, 20 micromolar MMACHC/CNCbl, 200 micromolar NADPH\ncross_nutrient: true\nevidence_location: Results: Figure 4; Materials and Methods: Decyanation of CNCbl\n[kim-2008-decyanation] Decyanation of vitamin B12 by a trafficking chaperone (2008). https://pubmed.ncbi.nlm.nih.gov/18779575/ DOI: 10.1073/pnas.0805989105","model_system":"Human purified-protein anaerobic reconstitution","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [kim-2008-decyanation] Decyanation of vitamin B12 by a trafficking chaperone (2008). https://pubmed.ncbi.nlm.nih.gov/18779575/ DOI: 10.1073/pnas.0805989105","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73145023-2982-5848-aff2-8d8875d6b9c5","stable_key":"import-7fc92b9e-9cbf-556e-8719-6b5244625250","title":"Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ad5b3a51d36e856aa7fdfd1cc23f690b094689bad87d28e1a621a54675a01c92","revision_id":"118bd616-0c13-549f-bfeb-bc439715b89c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}