Component

NADK

Human cytosolic NAD kinase; distinct from mitochondrial NADK2.

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. Purified full-length human NADK catalyzed NADP+ production from NAD+ in a coupled assay supplied with 5 mM MgATP.

    NADK → NADP+ source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    Mg-ATP supplies the phosphate donor for generating niacin-derived NADP+. NADP+ production is distinct from its later reduction to NADPH.
    evidence_span
    {"source_cache": "artifacts/niacin-redox-sources/nadk-human-2025.fulltext.txt", "locator": "Results", "start_char": 53525, "end_char": 54577, "file_sha256": "72c7b9625e1bf4137d6833adac6ae49fc108f2cd75bb4945c5b04d72a0355f9b", "text_sha256": "db49fc47ad605562e1c1cf93e1b39596505906831ae509e7ec0031485f84ba4f"}
    experimental_model
    Full-length recombinant human NADK; G6PD-coupled assay
    exposure
    5 mM MgATP; 0.1–2 mM NAD+; 1 mM additional MgCl2; 30°C
    limitations
    Scoped experimental observation; no dietary niacin or magnesium deficiency threshold or treatment benefit was tested.
    nutrient_topic
    Niacin research collection; topical membership is not evidence of a direct dietary effect. · Niacin (vitamin B3)
    organism
    Homo sapiens
    plain_language
    The cytosolic kinase makes the phosphorylated cofactor using magnesium-associated ATP.
    primary_references
    [nadk-human-2025] Cryo-EM structure and regulation of human NAD kinase. (2025). https://pubmed.ncbi.nlm.nih.gov/39854463/ DOI: 10.1126/sciadv.ads2664
    tissue_or_cell_type
    Purified enzyme

    Niacin: NAD metabolism, deficiency and nutrient interactions (2026-09-17) · lines 831–843

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Full-length recombinant human NADK; G6PD-coupled assay · source_derived_draft · unverified_draft

    ### b3-redox-nadk-mgatp Purified full-length human NADK catalyzed NADP+ production from NAD+ in a coupled assay supplied with 5 mM MgATP. Condition category: normal nutrient_topic: Niacin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The cytosolic kinase makes the phosphorylated cofactor using magnesium-associated ATP. organism: Homo sapiens tissue_or_cell_type: Purified enzyme experimental_model: Full-length recombinant human NADK; G6PD-coupled assay limitations: Scoped experimental observation; no dietary niacin or magnesium deficiency threshold or treatment benefit was tested. exposure: 5 mM MgATP; 0.1–2 mM NAD+; 1 mM additional MgCl2; 30°C cross_nutrient: Mg-ATP supplies the phosphate donor for generating niacin-derived NADP+. NADP+ production is distinct from its later reduction to NADPH. evidence_span: {"source_cache": "artifacts/niacin-redox-sources/nadk-human-2025.fulltext.txt", "locator": "Results", "start_char": 53525, "end_char": 54577, "file_sha256": "72c7b9625e1bf4137d6833adac6ae49fc108f2cd75bb4945c5b04d72a0355f9b", "text_sha256": "db49fc47ad605562e1c1cf93e1b39596505906831ae509e7ec0031485f84ba4f"} [nadk-human-2025] Cryo-EM structure and regulation of human NAD kinase. (2025). https://pubmed.ncbi.nlm.nih.gov/39854463/ DOI: 10.1126/sciadv.ads2664
    Complete structured claim and evidence

  2. The recombinant human NADK study did not detect phosphorylation of nicotinic acid adenine dinucleotide (NAAD).

    NADK → Nicotinic acid adenine dinucleotide source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/niacin-redox-sources/nadk-human-2001.abstract.txt", "locator": "Indexed abstract, substrate specificity", "start_char": 734, "end_char": 1005, "file_sha256": "98ea2e14dad1649e591f0c6420828328f84a7fe9e083e24b7379d6b680f11958", "text_sha256": "e61bb4892b61f0ed3a4b2eda8bc0131424a7854f7bd670fbd1544936b99c4d88"}
    experimental_model
    Recombinant human NADK
    exposure
    NAAD compared with NAD
    limitations
    Scoped experimental observation; no dietary niacin or magnesium deficiency threshold or treatment benefit was tested.
    nutrient_topic
    Niacin research collection; topical membership is not evidence of a direct dietary effect. · Niacin (vitamin B3)
    organism
    Homo sapiens
    plain_language
    Changing the nicotinamide group changes which nucleotide the kinase can use.
    primary_references
    [nadk-human-2001] Structural and functional characterization of human NAD kinase. (2001). https://pubmed.ncbi.nlm.nih.gov/11594753/ DOI: 10.1006/bbrc.2001.5735
    tissue_or_cell_type
    Purified enzyme

    Niacin: NAD metabolism, deficiency and nutrient interactions (2026-09-17) · lines 858–869

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Recombinant human NADK · source_derived_draft · unverified_draft

    ### b3-redox-nadk-naad-negative The recombinant human NADK study did not detect phosphorylation of nicotinic acid adenine dinucleotide (NAAD). Condition category: normal nutrient_topic: Niacin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Changing the nicotinamide group changes which nucleotide the kinase can use. organism: Homo sapiens tissue_or_cell_type: Purified enzyme experimental_model: Recombinant human NADK limitations: Scoped experimental observation; no dietary niacin or magnesium deficiency threshold or treatment benefit was tested. exposure: NAAD compared with NAD evidence_span: {"source_cache": "artifacts/niacin-redox-sources/nadk-human-2001.abstract.txt", "locator": "Indexed abstract, substrate specificity", "start_char": 734, "end_char": 1005, "file_sha256": "98ea2e14dad1649e591f0c6420828328f84a7fe9e083e24b7379d6b680f11958", "text_sha256": "e61bb4892b61f0ed3a4b2eda8bc0131424a7854f7bd670fbd1544936b99c4d88"} [nadk-human-2001] Structural and functional characterization of human NAD kinase. (2001). https://pubmed.ncbi.nlm.nih.gov/11594753/ DOI: 10.1006/bbrc.2001.5735
    Complete structured claim and evidence

  3. Cryo-EM resolved full-length human NADK as a tetramer with active sites spanning adjacent monomers.

    NADK → NADK source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/niacin-redox-sources/nadk-human-2025.fulltext.txt", "locator": "Results", "start_char": 4029, "end_char": 5074, "file_sha256": "72c7b9625e1bf4137d6833adac6ae49fc108f2cd75bb4945c5b04d72a0355f9b", "text_sha256": "e3b82e8f1acea39f7023ee7e6d83f6631d0c00ec23d5f98d96cef0707dcaa34c"}
    experimental_model
    Full-length NADK purified from HEK293 cells; cryo-EM
    exposure
    Apo protein structure
    limitations
    Scoped experimental observation; no dietary niacin or magnesium deficiency threshold or treatment benefit was tested.
    nutrient_topic
    Niacin research collection; topical membership is not evidence of a direct dietary effect. · Niacin (vitamin B3)
    organism
    Homo sapiens
    plain_language
    The cytosolic enzyme operates in a four-subunit assembly.
    primary_references
    [nadk-human-2025] Cryo-EM structure and regulation of human NAD kinase. (2025). https://pubmed.ncbi.nlm.nih.gov/39854463/ DOI: 10.1126/sciadv.ads2664
    tissue_or_cell_type
    Purified protein

    Niacin: NAD metabolism, deficiency and nutrient interactions (2026-09-17) · lines 845–856

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Full-length NADK purified from HEK293 cells; cryo-EM · source_derived_draft · unverified_draft

    ### b3-redox-nadk-tetramer Cryo-EM resolved full-length human NADK as a tetramer with active sites spanning adjacent monomers. Condition category: normal nutrient_topic: Niacin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The cytosolic enzyme operates in a four-subunit assembly. organism: Homo sapiens tissue_or_cell_type: Purified protein experimental_model: Full-length NADK purified from HEK293 cells; cryo-EM limitations: Scoped experimental observation; no dietary niacin or magnesium deficiency threshold or treatment benefit was tested. exposure: Apo protein structure evidence_span: {"source_cache": "artifacts/niacin-redox-sources/nadk-human-2025.fulltext.txt", "locator": "Results", "start_char": 4029, "end_char": 5074, "file_sha256": "72c7b9625e1bf4137d6833adac6ae49fc108f2cd75bb4945c5b04d72a0355f9b", "text_sha256": "e3b82e8f1acea39f7023ee7e6d83f6631d0c00ec23d5f98d96cef0707dcaa34c"} [nadk-human-2025] Cryo-EM structure and regulation of human NAD kinase. (2025). https://pubmed.ncbi.nlm.nih.gov/39854463/ DOI: 10.1126/sciadv.ads2664
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards