Component
Mustard myrosinase, study-resolved enzyme
Mustard myrosinase, study-resolved enzyme. Species, exposure and limitations are retained in each linked claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Ascorbate accelerated hydrolysis of the glycosyl-enzyme intermediate and occupied a position supporting its role as the catalytic base.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/sulforaphane-research/10978344.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "ed373ac9a91fa7b03d25c21c32adaf57dd2f0f9b65e1f99e9da49aab4c22b0aa", "start_char": 0, "end_char": 1511, "text_sha256": "ed373ac9a91fa7b03d25c21c32adaf57dd2f0f9b65e1f99e9da49aab4c22b0aa"}
- experimental_model
- High-resolution crystallography and reactivation kinetics
- exposure
- Ascorbate with substrate analogues and a trapped glycosyl-enzyme intermediate
- limitations
- Plant enzyme chemistry; does not show that vitamin C pills increase systemic sulforaphane in people.
- nutrient_topic
- Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
- organism
- Mustard plant myrosinase
- plain_language
- Vitamin C can help the plant enzyme carry out its reaction.
- primary_references
- [sulforaphane-p10978344] High resolution X-ray crystallography shows that ascorbate is a cofactor for myrosinase and substitutes for the function of the catalytic base. (2000). https://pubmed.ncbi.nlm.nih.gov/10978344/ DOI: 10.1074/jbc.m006796200
- tissue_or_cell_type
- Enzyme active site
Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 190–201
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · High-resolution crystallography and reactivation kinetics · source_derived_draft · unverified_draft
### sulforaphane-ascorbate-myrosinase Ascorbate accelerated hydrolysis of the glycosyl-enzyme intermediate and occupied a position supporting its role as the catalytic base. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: Vitamin C can help the plant enzyme carry out its reaction. organism: Mustard plant myrosinase tissue_or_cell_type: Enzyme active site experimental_model: High-resolution crystallography and reactivation kinetics limitations: Plant enzyme chemistry; does not show that vitamin C pills increase systemic sulforaphane in people. exposure: Ascorbate with substrate analogues and a trapped glycosyl-enzyme intermediate evidence_span: {"source_cache": "artifacts/sulforaphane-research/10978344.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "ed373ac9a91fa7b03d25c21c32adaf57dd2f0f9b65e1f99e9da49aab4c22b0aa", "start_char": 0, "end_char": 1511, "text_sha256": "ed373ac9a91fa7b03d25c21c32adaf57dd2f0f9b65e1f99e9da49aab4c22b0aa"} [sulforaphane-p10978344] High resolution X-ray crystallography shows that ascorbate is a cofactor for myrosinase and substitutes for the function of the catalytic base. (2000). https://pubmed.ncbi.nlm.nih.gov/10978344/ DOI: 10.1074/jbc.m006796200
Complete structured claim and evidence
Where it participates (unsigned role)
Myrosinase increased mean urinary-metabolite-based recovery from 18.6% to 39.8%, and early conversion during the first eight hours from 8.0% to 25.4%.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/sulforaphane-research/41692762.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "67e337141c8012ea573d9720d5e4774bfdd1ae098c056b68991752169fbdb32a", "start_char": 0, "end_char": 1248, "text_sha256": "67e337141c8012ea573d9720d5e4774bfdd1ae098c056b68991752169fbdb32a"}
- experimental_model
- Randomized double-blind crossover single-dose study
- exposure
- Broccoli seed glucoraphanin with versus without mustard myrosinase; both arms contained ascorbic acid
- limitations
- Both arms included ascorbic acid, so its independent benefit cannot be inferred; conversion biomarker is not clinical efficacy.
- nutrient_topic
- Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
- organism
- Human, 16 adults, nine women and seven men
- plain_language
- A recent controlled product comparison confirms the importance of enzyme activity.
- primary_references
- [sulforaphane-p41692762] Exogenous myrosinase from mustard seed increases bioavailability of sulforaphane from a glucoraphanin-rich broccoli seed extract in a randomized clinical study. (2026). https://pubmed.ncbi.nlm.nih.gov/41692762/ DOI: 10.1038/s41598-026-39389-4
- tissue_or_cell_type
- Urinary metabolites and fecal microbial genes
Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 996–1007
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Randomized double-blind crossover single-dose study · source_derived_draft · unverified_draft
### sulforaphane-mustard-seed-trial Myrosinase increased mean urinary-metabolite-based recovery from 18.6% to 39.8%, and early conversion during the first eight hours from 8.0% to 25.4%. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: A recent controlled product comparison confirms the importance of enzyme activity. organism: Human, 16 adults, nine women and seven men tissue_or_cell_type: Urinary metabolites and fecal microbial genes experimental_model: Randomized double-blind crossover single-dose study limitations: Both arms included ascorbic acid, so its independent benefit cannot be inferred; conversion biomarker is not clinical efficacy. exposure: Broccoli seed glucoraphanin with versus without mustard myrosinase; both arms contained ascorbic acid evidence_span: {"source_cache": "artifacts/sulforaphane-research/41692762.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "67e337141c8012ea573d9720d5e4774bfdd1ae098c056b68991752169fbdb32a", "start_char": 0, "end_char": 1248, "text_sha256": "67e337141c8012ea573d9720d5e4774bfdd1ae098c056b68991752169fbdb32a"} [sulforaphane-p41692762] Exogenous myrosinase from mustard seed increases bioavailability of sulforaphane from a glucoraphanin-rich broccoli seed extract in a randomized clinical study. (2026). https://pubmed.ncbi.nlm.nih.gov/41692762/ DOI: 10.1038/s41598-026-39389-4
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.