Component

Mouse thiamine pyrophosphokinase 1

Mus musculus TPK1 used in recombinant structural and kinetic experiments.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. Purified mouse TPK1 formed ATP and thiamine from ThDP plus AMP under high-AMP, magnesium-containing conditions.

    Mouse thiamine pyrophosphokinase 1 → ATP source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    Mg dependence remains distinct from physiological net reaction direction.
    evidence-scope
    Recombinant enzyme
    evidence_locator
    Reaction definition and sections 2.2 and 3.3
    evidence_spans
    [{"source_document": "artifacts/thiamine_transport_sources/sambon-2022-tpk1-source-record.json", "source_field": "resultList.result[0].abstractText", "start_char": 0, "end_char": 1689}]
    experimental_model
    Purified recombinant mouse TPK1; forward and reverse reactions.
    limitations
    High AMP/Mg requirements do not establish meaningful reverse flux in humans.
    nutrient_topic
    Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
    organism
    Mus musculus
    plain_language
    The activation chemistry can run backward experimentally.
    primary_references
    [sambon-2022-tpk1] Product inhibition of mammalian thiamine pyrophosphokinase is an important mechanism for maintaining thiamine diphosphate homeostasis (2022). https://doi.org/10.1016/j.bbagen.2021.130071 DOI: 10.1016/j.bbagen.2021.130071
    tissue_or_cell_type
    Recombinant enzyme

    Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 373–386

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purified recombinant mouse TPK1; forward and reverse reactions. · source_derived_draft · unverified_draft

    ### b1-mouse-tpk1-reverse-reaction Purified mouse TPK1 formed ATP and thiamine from ThDP plus AMP under high-AMP, magnesium-containing conditions. Condition category: normal nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: The activation chemistry can run backward experimentally. organism: Mus musculus tissue_or_cell_type: Recombinant enzyme experimental_model: Purified recombinant mouse TPK1; forward and reverse reactions. limitations: High AMP/Mg requirements do not establish meaningful reverse flux in humans. cross_nutrient: Mg dependence remains distinct from physiological net reaction direction. evidence_spans: [{"source_document": "artifacts/thiamine_transport_sources/sambon-2022-tpk1-source-record.json", "source_field": "resultList.result[0].abstractText", "start_char": 0, "end_char": 1689}] evidence_locator: Reaction definition and sections 2.2 and 3.3 evidence-scope: Recombinant enzyme [sambon-2022-tpk1] Product inhibition of mammalian thiamine pyrophosphokinase is an important mechanism for maintaining thiamine diphosphate homeostasis (2022). https://doi.org/10.1016/j.bbagen.2021.130071 DOI: 10.1016/j.bbagen.2021.130071
    Complete structured claim and evidence

What acts on it

  1. Mouse TPK structures place Mg2+ adjacent to thiamine/ThDP and coordinated by active-site aspartate residues.

    Mg2+ → Mouse thiamine pyrophosphokinase 1 source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    Magnesium availability supports vitamin B1 activation or cofactor use in the specified preparation; this does not establish a dietary threshold or universal treatment failure.
    experimental_model
    Recombinant mouse TPK crystal soaking with thiamine or ThDP and magnesium.
    limitations
    Crystal packing may affect oligomer state; nucleotide analog density was not confidently assigned.
    nutrient_topic
    Magnesium research collection; topical membership is not evidence of a direct dietary effect. · Magnesium
    organism
    Mus musculus
    plain_language
    Magnesium participates in the enzyme pocket that handles vitamin B1 phosphorylation.
    primary_references
    [liu-2011-tpk-structure] A new crystal form of mouse thiamin pyrophosphokinase (2011). https://pmc.ncbi.nlm.nih.gov/articles/PMC3088427/
    tissue_or_cell_type
    Recombinant protein crystals

    Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17) · lines 505–515

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Recombinant mouse TPK crystal soaking with thiamine or ThDP and magnesium. · source_derived_draft · unverified_draft

    ### mg-mouse-tpk-metal-site Mouse TPK structures place Mg2+ adjacent to thiamine/ThDP and coordinated by active-site aspartate residues. Condition category: normal nutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect. plain_language: Magnesium participates in the enzyme pocket that handles vitamin B1 phosphorylation. organism: Mus musculus tissue_or_cell_type: Recombinant protein crystals experimental_model: Recombinant mouse TPK crystal soaking with thiamine or ThDP and magnesium. limitations: Crystal packing may affect oligomer state; nucleotide analog density was not confidently assigned. cross_nutrient: Magnesium availability supports vitamin B1 activation or cofactor use in the specified preparation; this does not establish a dietary threshold or universal treatment failure. [liu-2011-tpk-structure] A new crystal form of mouse thiamin pyrophosphokinase (2011). https://pmc.ncbi.nlm.nih.gov/articles/PMC3088427/
    Complete structured claim and evidence

  2. Recombinant mouse TPK1 showed an Mg optimum followed by inhibition as Mg was increased at fixed ATP.

    Mg2+ → Mouse thiamine pyrophosphokinase 1 source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    Magnesium availability supports vitamin B1 activation or cofactor use in the specified preparation; this does not establish a dietary threshold or universal treatment failure.
    experimental_model
    Purified recombinant mouse TPK1; forward and reverse reaction kinetics.
    limitations
    ATP concentration shifted the optimum; in vitro values are not intake targets.
    nutrient_topic
    Magnesium research collection; topical membership is not evidence of a direct dietary effect. · Magnesium
    organism
    Mus musculus
    plain_language
    More magnesium did not continuously increase B1 activation in this assay.
    primary_references
    [sambon-2022-tpk1] Product inhibition of mammalian thiamine pyrophosphokinase is an important mechanism for maintaining thiamine diphosphate homeostasis (2022). https://doi.org/10.1016/j.bbagen.2021.130071 DOI: 10.1016/j.bbagen.2021.130071
    tissue_or_cell_type
    Purified recombinant enzyme

    Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17) · lines 517–527

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purified recombinant mouse TPK1; forward and reverse reaction kinetics. · source_derived_draft · unverified_draft

    ### mg-mouse-tpk-nonmonotonic Recombinant mouse TPK1 showed an Mg optimum followed by inhibition as Mg was increased at fixed ATP. Condition category: normal nutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect. plain_language: More magnesium did not continuously increase B1 activation in this assay. organism: Mus musculus tissue_or_cell_type: Purified recombinant enzyme experimental_model: Purified recombinant mouse TPK1; forward and reverse reaction kinetics. limitations: ATP concentration shifted the optimum; in vitro values are not intake targets. cross_nutrient: Magnesium availability supports vitamin B1 activation or cofactor use in the specified preparation; this does not establish a dietary threshold or universal treatment failure. [sambon-2022-tpk1] Product inhibition of mammalian thiamine pyrophosphokinase is an important mechanism for maintaining thiamine diphosphate homeostasis (2022). https://doi.org/10.1016/j.bbagen.2021.130071 DOI: 10.1016/j.bbagen.2021.130071
    Complete structured claim and evidence

  3. Accumulated ThDP inhibited the forward reaction of purified mouse TPK1.

    Thiamine diphosphate → Mouse thiamine pyrophosphokinase 1 source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    Magnesium availability supports vitamin B1 activation or cofactor use in the specified preparation; this does not establish a dietary threshold or universal treatment failure.
    experimental_model
    Purified recombinant mouse TPK1; forward and reverse reaction kinetics.
    limitations
    Product inhibition is a separate limit from Mg shortage.
    nutrient_topic
    Magnesium research collection; topical membership is not evidence of a direct dietary effect. · Magnesium
    organism
    Mus musculus
    plain_language
    B1 activation has product feedback even when ATP and magnesium are supplied.
    primary_references
    [sambon-2022-tpk1] Product inhibition of mammalian thiamine pyrophosphokinase is an important mechanism for maintaining thiamine diphosphate homeostasis (2022). https://doi.org/10.1016/j.bbagen.2021.130071 DOI: 10.1016/j.bbagen.2021.130071
    tissue_or_cell_type
    Purified recombinant enzyme

    Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17) · lines 529–539

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purified recombinant mouse TPK1; forward and reverse reaction kinetics. · source_derived_draft · unverified_draft

    ### mg-thdp-feedback-on-tpk Accumulated ThDP inhibited the forward reaction of purified mouse TPK1. Condition category: normal nutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect. plain_language: B1 activation has product feedback even when ATP and magnesium are supplied. organism: Mus musculus tissue_or_cell_type: Purified recombinant enzyme experimental_model: Purified recombinant mouse TPK1; forward and reverse reaction kinetics. limitations: Product inhibition is a separate limit from Mg shortage. cross_nutrient: Magnesium availability supports vitamin B1 activation or cofactor use in the specified preparation; this does not establish a dietary threshold or universal treatment failure. [sambon-2022-tpk1] Product inhibition of mammalian thiamine pyrophosphokinase is an important mechanism for maintaining thiamine diphosphate homeostasis (2022). https://doi.org/10.1016/j.bbagen.2021.130071 DOI: 10.1016/j.bbagen.2021.130071
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards