{"id":"851370b0-6514-59b3-8a86-7fe6b3542e5b","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-mouse-tpk-metal-site","predicate":"coordinates-with","statement":"Mouse TPK structures place Mg2+ adjacent to thiamine/ThDP and coordinated by active-site aspartate residues.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a66bf546-5528-598f-adf3-a26be0da4b19","mechanism_event_label":"Magnesium participates in the enzyme pocket that handles vitamin B1 phosphorylation.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"692f8f7a-32ba-58ff-9242-0784d97f9bff","slug":"mouse-tpk1","display_name":"Mouse thiamine pyrophosphokinase 1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a66bf546-5528-598f-adf3-a26be0da4b19","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-mouse-tpk-metal-site-event","event_type":"biochemical_relationship","label":"Magnesium participates in the enzyme pocket that handles vitamin B1 phosphorylation.","description":"Mouse TPK structures place Mg2+ adjacent to thiamine/ThDP and coordinated by active-site aspartate residues.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"bound substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"187db168-8028-5ce6-9f8b-4bc61ebad1a0","slug":"thiamine-diphosphate","display_name":"Thiamine diphosphate","entity_type_key":"small_molecule"},"role":"bound product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"692f8f7a-32ba-58ff-9242-0784d97f9bff","slug":"mouse-tpk1","display_name":"Mouse thiamine pyrophosphokinase 1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Magnesium availability supports vitamin B1 activation or cofactor use in the specified preparation; this does not establish a dietary threshold or universal treatment failure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant mouse TPK crystal soaking with thiamine or ThDP and magnesium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Crystal packing may affect oligomer state; nucleotide analog density was not confidently assigned.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Magnesium participates in the enzyme pocket that handles vitamin B1 phosphorylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[liu-2011-tpk-structure] A new crystal form of mouse thiamin pyrophosphokinase (2011). https://pmc.ncbi.nlm.nih.gov/articles/PMC3088427/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Recombinant protein crystals","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ccd544e7-9128-573a-8fe1-79d646fa837a","evidence_kind":"source_excerpt","locator":"Lines 505-515","start_line":505,"end_line":515,"excerpt":"### mg-mouse-tpk-metal-site\nMouse TPK structures place Mg2+ adjacent to thiamine/ThDP and coordinated by active-site aspartate residues.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Magnesium participates in the enzyme pocket that handles vitamin B1 phosphorylation.\norganism: Mus musculus\ntissue_or_cell_type: Recombinant protein crystals\nexperimental_model: Recombinant mouse TPK crystal soaking with thiamine or ThDP and magnesium.\nlimitations: Crystal packing may affect oligomer state; nucleotide analog density was not confidently assigned.\ncross_nutrient: Magnesium availability supports vitamin B1 activation or cofactor use in the specified preparation; this does not establish a dietary threshold or universal treatment failure.\n[liu-2011-tpk-structure] A new crystal form of mouse thiamin pyrophosphokinase (2011). https://pmc.ncbi.nlm.nih.gov/articles/PMC3088427/","model_system":"Recombinant mouse TPK crystal soaking with thiamine or ThDP and magnesium.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [liu-2011-tpk-structure] A new crystal form of mouse thiamin pyrophosphokinase (2011). https://pmc.ncbi.nlm.nih.gov/articles/PMC3088427/","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}