Component
Mouse ergothioneine transporter Octn1 / Slc22a4
Mouse transporter; knockout effects are not a dietary deficiency model.
3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Slc22a4 knockout impaired but did not abolish ergothioneine uptake by isolated mouse mitochondria.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_access
- Primary indexed abstract; numerical exposures and tissue-specific methods not available in the abstract.
- experimental_condition
- non-knockout mitochondrial comparison knockout · Mouse ergothioneine transporter Octn1 / Slc22a4 Condition belongs to the full experimental contrast; do not separate a joint intervention.
- experimental_contrast
- {"intervention": "Octn1/Slc22a4 knockout", "comparator": "non-knockout mitochondrial comparison", "endpoint": "mitochondrial ergothioneine uptake", "effect_direction": "decrease", "combination": "single", "conditions": [{"entity_slug": "mouse-slc22a4", "state": "knockout"}]} Explicit extracted experimental comparison; source-derived draft.
- experimental_model
- Mitochondria isolated from Octn1-knockout mouse tissues.
- interpretation_status
- Source-derived research curation; not independent raw-data verification.
- limitations
- Residual uptake does not prove which alternative transporter operates, nor that OCTN1 normally resides on the mitochondrial membrane.
- nutrient_topic
- Ergothioneine mitochondrial supplement; shared molecular requirements are not demonstrated dietary interactions. · L-Ergothioneine
- plain_language
- An alternative mitochondrial uptake route remains possible.
- primary_references
- Fong et al. Ergothioneine and mitochondria: An important protective mechanism? DOI 10.1016/j.bbrc.2024.150269; PMID 38909533; https://pubmed.ncbi.nlm.nih.gov/38909533/
- source_locator
- Abstract, knockout comparison
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Ergothioneine: mitochondrial transport, MPST and sulfur-handling dependencies (2026-10-02) · lines 33–33
Original AI-assisted curation of five primary studies with publication identifiers, experimental locators and access limitations. Additive chapter supplement, not publisher full text. · supports · Mitochondria isolated from Octn1-knockout mouse tissues. · source_derived_draft · unverified_draft
Slc22a4 knockout impaired but did not abolish ergothioneine uptake by isolated mouse mitochondria.
Complete structured claim and evidence
What acts on it
Muscle PGC-1alpha overexpression increased Slc22a4 mRNA and protein in mice.
Experimental context and source evidence
- evidence_access
- Publisher abstract/introduction plus the authors' article and supplementary legends reproduced in an indexed document. This is source-derived extraction, not raw-data verification. Main-text OCR corrupts some micro-unit symbols; ambiguous doses and binding constants are deliberately not transcribed.
- experimental_condition
- wild-type mice muscle overexpression · Mouse PGC-1alpha / Ppargc1a Condition belongs to the full experimental contrast; do not separate a joint intervention.
- experimental_contrast
- {"intervention": "muscle-specific PGC-1alpha overexpression", "comparator": "wild-type mice", "endpoint": "Slc22a4 mRNA and protein", "effect_direction": "increase", "combination": "single", "conditions": [{"entity_slug": "mouse-ppargc1a", "state": "muscle overexpression"}]} Explicit extracted experimental comparison; source-derived draft.
- experimental_model
- MCK-PGC-1alpha mice, gastrocnemius muscle, versus wild-type controls.
- interpretation_status
- Source-derived research curation; not independent raw-data verification.
- limitations
- Overexpression differs from physiological exercise and human diet; increased transporter abundance is not itself a measured transport flux.
- nutrient_topic
- Ergothioneine mitochondrial supplement; shared molecular requirements are not demonstrated dietary interactions. · L-Ergothioneine
- plain_language
- A regulatory programme can alter transporter abundance.
- primary_references
- Sprenger et al. Ergothioneine controls mitochondrial function and exercise performance via direct activation of MPST. DOI 10.1016/j.cmet.2025.01.024; PMID 39965563; https://pubmed.ncbi.nlm.nih.gov/39965563/
- source_locator
- Figure 2D-E
Ergothioneine: mitochondrial transport, MPST and sulfur-handling dependencies (2026-10-02) · lines 97–97
Original AI-assisted curation of five primary studies with publication identifiers, experimental locators and access limitations. Additive chapter supplement, not publisher full text. · supports · MCK-PGC-1alpha mice, gastrocnemius muscle, versus wild-type controls. · source_derived_draft · unverified_draft
Muscle PGC-1alpha overexpression increased Slc22a4 mRNA and protein in mice.
Complete structured claim and evidence
Where it participates (unsigned role)
PGC-1alpha-overexpressing mouse muscle contained more ergothioneine than control muscle.
Experimental context and source evidence
- evidence_access
- Publisher abstract/introduction plus the authors' article and supplementary legends reproduced in an indexed document. This is source-derived extraction, not raw-data verification. Main-text OCR corrupts some micro-unit symbols; ambiguous doses and binding constants are deliberately not transcribed.
- experimental_condition
- wild-type mice muscle overexpression · Mouse PGC-1alpha / Ppargc1a Condition belongs to the full experimental contrast; do not separate a joint intervention.
- experimental_contrast
- {"intervention": "muscle-specific PGC-1alpha overexpression", "comparator": "wild-type mice", "endpoint": "whole-muscle ergothioneine concentration", "effect_direction": "increase", "combination": "single", "conditions": [{"entity_slug": "mouse-ppargc1a", "state": "muscle overexpression"}]} Explicit extracted experimental comparison; source-derived draft.
- experimental_model
- MCK-PGC-1alpha mice; whole gastrocnemius metabolomics.
- interpretation_status
- Source-derived research curation; not independent raw-data verification.
- limitations
- Whole-muscle concentration is not the isolated mitochondrial pool. This does not establish that transporter upregulation is the only causal route.
- nutrient_topic
- Ergothioneine mitochondrial supplement; shared molecular requirements are not demonstrated dietary interactions. · L-Ergothioneine
- plain_language
- The muscle pool changed alongside transporter expression.
- primary_references
- Sprenger et al. Ergothioneine controls mitochondrial function and exercise performance via direct activation of MPST. DOI 10.1016/j.cmet.2025.01.024; PMID 39965563; https://pubmed.ncbi.nlm.nih.gov/39965563/
- source_locator
- Figure 2F
Ergothioneine: mitochondrial transport, MPST and sulfur-handling dependencies (2026-10-02) · lines 105–105
Original AI-assisted curation of five primary studies with publication identifiers, experimental locators and access limitations. Additive chapter supplement, not publisher full text. · supports · MCK-PGC-1alpha mice; whole gastrocnemius metabolomics. · source_derived_draft · unverified_draft
PGC-1alpha-overexpressing mouse muscle contained more ergothioneine than control muscle.
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.