{"id":"b660fa27-be6e-514f-99b8-f6d4abb2a53a","stable_key":"920dfea8-62e3-5bd4-8b18-d19f86eb8295:egt-octn1-residual-mito-uptake","predicate":"loss_reduces_but_does_not_abolish","statement":"Slc22a4 knockout impaired but did not abolish ergothioneine uptake by isolated mouse mitochondria.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"dde02edb-31ee-56dd-b71b-2c81701e060d","mechanism_event_label":"An alternative mitochondrial uptake route remains possible.","subject":{"id":"3d681cdf-2324-5453-863a-83a1091852b2","slug":"mouse-slc22a4","display_name":"Mouse ergothioneine transporter Octn1 / Slc22a4","entity_type_key":"protein"},"object":{"id":"123d249e-a7d0-53d8-b48a-47cf557dfe83","slug":"mouse-mitochondrial-ergothioneine-uptake","display_name":"Ergothioneine uptake into isolated mouse mitochondria","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"dde02edb-31ee-56dd-b71b-2c81701e060d","stable_key":"920dfea8-62e3-5bd4-8b18-d19f86eb8295:egt-octn1-residual-mito-uptake-event","event_type":"biochemical_relationship","label":"An alternative mitochondrial uptake route remains possible.","description":"Slc22a4 knockout impaired but did not abolish ergothioneine uptake by isolated mouse mitochondria.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3d681cdf-2324-5453-863a-83a1091852b2","slug":"mouse-slc22a4","display_name":"Mouse ergothioneine transporter Octn1 / Slc22a4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"123d249e-a7d0-53d8-b48a-47cf557dfe83","slug":"mouse-mitochondrial-ergothioneine-uptake","display_name":"Ergothioneine uptake into isolated mouse mitochondria","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"transported compound","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary indexed abstract; numerical exposures and tissue-specific methods not available in the abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"knockout","comparator":"non-knockout mitochondrial comparison","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"mouse-slc22a4","display_name":"Mouse ergothioneine transporter Octn1 / Slc22a4","entity_type_key":"protein"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Octn1/Slc22a4 knockout\", \"comparator\": \"non-knockout mitochondrial comparison\", \"endpoint\": \"mitochondrial ergothioneine uptake\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": [{\"entity_slug\": \"mouse-slc22a4\", \"state\": \"knockout\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Mitochondria isolated from Octn1-knockout mouse tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Residual uptake does not prove which alternative transporter operates, nor that OCTN1 normally resides on the mitochondrial membrane.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine mitochondrial supplement; shared molecular requirements are not demonstrated dietary interactions.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An alternative mitochondrial uptake route remains possible.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fong et al. Ergothioneine and mitochondria: An important protective mechanism? DOI 10.1016/j.bbrc.2024.150269; PMID 38909533; https://pubmed.ncbi.nlm.nih.gov/38909533/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Abstract, knockout comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"331ddd6b-d0e5-566a-8491-7ff5e1de4703","evidence_kind":"source_excerpt","locator":"Lines 33-33","start_line":33,"end_line":33,"excerpt":"Slc22a4 knockout impaired but did not abolish ergothioneine uptake by isolated mouse mitochondria.","model_system":"Mitochondria isolated from Octn1-knockout mouse tissues.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Evidence points to this original curation statement. 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