Component
Mouse BV2 nitrite release
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Shikimic acid lowered the LPS-associated nitrite readout.
Experimental context and source evidence
- evidence_access
- Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.
- experimental_contrast
- {"intervention": "Shikimic acid in the endpoint-specific assay", "comparator": "Matched assay without shikimic acid", "endpoint": "Shikimic acid lowered the LPS-associated nitrite readout.", "effect_direction": "decrease", "combination": "single", "conditions": []} Explicit extracted experimental comparison; source-derived draft.
- experimental_model
- Mouse BV2 cells; 5/10 µM SA, LPS 1 µg/mL, 24 h for inflammatory endpoints; separate viability test.
- interpretation_status
- Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
- limitations
- Assay-specific redox or viability signal; not a unique ROS identity or direct scavenging mechanism.
- plain_language
- Shikimic acid lowered the LPS-associated nitrite readout.
- primary_references
- Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced <i>in vitro</i> and <i>in vivo</i> model. | 2023 | DOI 10.3389/fphar.2023.1265571 | PMID 38026972 | https://pubmed.ncbi.nlm.nih.gov/38026972/ | https://doi.org/10.3389/fphar.2023.1265571 | https://pmc.ncbi.nlm.nih.gov/articles/PMC10652795/
- source_locator
- Reviewed reference lines 59-59; exact primary location described in quoted passage where extracted.
Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 59–59
Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Mouse BV2 cells; 5/10 µM SA, LPS 1 µg/mL, 24 h for inflammatory endpoints; separate viability test. · source_derived_draft · unverified_draft
**Microglial redox responses were measured at micromolar exposure.** In mouse BV2 cells, 5 or 10 µM shikimic acid with 1 µg/mL LPS for 24 h decreased nitrite and DCFH-DA oxidation readouts; concentrations up to 10 µM did not significantly reduce the reported CCK8 viability signal. These are assays of inflammatory/redox response and metabolic viability, not measurements of a unique reactive-oxygen species or proof of direct radical scavenging. [Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced <i>in vitro</i> and <i>in vivo</i> model.](https://pubmed.ncbi.nlm.nih.gov/38026972/)
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.