{"id":"966d2006-34d3-5413-966b-03fc12d5e9e6","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:bv2-nitrite","predicate":"decreases_in_recorded_experiment","statement":"Shikimic acid lowered the LPS-associated nitrite readout.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"73f82269-65b9-542d-8662-5aee800d761b","mechanism_event_label":"Shikimic acid lowered the LPS-associated nitrite readout.","subject":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"object":{"id":"675158bf-1619-5b6e-b3f4-5df726aacd4a","slug":"mouse-bv2-nitrite","display_name":"Mouse BV2 nitrite release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"73f82269-65b9-542d-8662-5aee800d761b","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:bv2-nitrite-event","event_type":"experimental_observation","label":"Shikimic acid lowered the LPS-associated nitrite readout.","description":"**Microglial redox responses were measured at micromolar exposure.** In mouse BV2 cells, 5 or 10 µM shikimic acid with 1 µg/mL LPS for 24 h decreased nitrite and DCFH-DA oxidation readouts; concentrations up to 10 µM did not significantly reduce the reported CCK8 viability signal. These are assays of inflammatory/redox response and metabolic viability, not measurements of a unique reactive-oxygen species or proof of direct radical scavenging. [Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced <i>in vitro</i> and <i>in vivo</i> model.](https://pubmed.ncbi.nlm.nih.gov/38026972/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Shikimic acid in the endpoint-specific assay","sequence_order":0,"notes":""},{"entity":{"id":"675158bf-1619-5b6e-b3f4-5df726aacd4a","slug":"mouse-bv2-nitrite","display_name":"Mouse BV2 nitrite release","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Shikimic acid in the endpoint-specific assay\", \"comparator\": \"Matched assay without shikimic acid\", \"endpoint\": \"Shikimic acid lowered the LPS-associated nitrite readout.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Mouse BV2 cells; 5/10 µM SA, LPS 1 µg/mL, 24 h for inflammatory endpoints; separate viability test.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay-specific redox or viability signal; not a unique ROS identity or direct scavenging mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Shikimic acid lowered the LPS-associated nitrite readout.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced <i>in vitro</i> and <i>in vivo</i> model. | 2023 | DOI 10.3389/fphar.2023.1265571 | PMID 38026972 | https://pubmed.ncbi.nlm.nih.gov/38026972/ | https://doi.org/10.3389/fphar.2023.1265571 | https://pmc.ncbi.nlm.nih.gov/articles/PMC10652795/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 59-59; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a5958d06-da54-51ee-87bf-1bd29ca8c26c","evidence_kind":"source_excerpt","locator":"Lines 59-59","start_line":59,"end_line":59,"excerpt":"**Microglial redox responses were measured at micromolar exposure.** In mouse BV2 cells, 5 or 10 µM shikimic acid with 1 µg/mL LPS for 24 h decreased nitrite and DCFH-DA oxidation readouts; concentrations up to 10 µM did not significantly reduce the reported CCK8 viability signal. These are assays of inflammatory/redox response and metabolic viability, not measurements of a unique reactive-oxygen species or proof of direct radical scavenging. [Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced <i>in vitro</i> and <i>in vivo</i> model.](https://pubmed.ncbi.nlm.nih.gov/38026972/)","model_system":"Mouse BV2 cells; 5/10 µM SA, LPS 1 µg/mL, 24 h for inflammatory endpoints; separate viability test.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e6ae59de-0369-5c2f-8262-57d91302671c","stable_key":"import-31b1baa4-4113-5541-b9e7-fe44a5253a07","title":"Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"95b1f9e9577661312d67f36e156d2e49326b207f296c1c0e801a5b007fd8e283","revision_id":"cd3237f1-131a-557d-84b5-7543259807b0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}