Component
Mouse AML12 mitochondrial respiration
Context-specific entity; species, compartment and exposure are stated on each claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Dohh siRNA prevented spermidine rescue of mitochondrial protein expression and respiration under fatty-acid stress.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_access
- Primary full text
- experimental_model
- Mouse AML12 cells; genetic knockdown and rescue design.
- limitations
- Does not imply that all low DOHH activity is caused by iron deficiency.
- nutrient_topic
- Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Spermidine
- plain_language
- More spermidine could not bypass the missing enzyme.
- primary_references
- Spermidine-mediated hypusination of translation factor EIF5A improves mitochondrial fatty acid oxidation and prevents non-alcoholic steatohepatitis progression. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36057633/ · DOI 10.1038/s41467-022-32788-x
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19) · lines 310–316
AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Mouse AML12 cells; genetic knockdown and rescue design. · source_derived_draft · unverified_draft
## spermidine-liver-dohh-failure More spermidine could not bypass the missing enzyme. Dohh siRNA prevented spermidine rescue of mitochondrial protein expression and respiration under fatty-acid stress. Model: Mouse AML12 cells; genetic knockdown and rescue design. Limitations: Does not imply that all low DOHH activity is caused by iron deficiency. Evidence access: Primary full text Spermidine-mediated hypusination of translation factor EIF5A improves mitochondrial fatty acid oxidation and prevents non-alcoholic steatohepatitis progression. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36057633/ · DOI 10.1038/s41467-022-32788-x
Complete structured claim and evidenceSpermidine partially restored protein synthesis and mitochondrial respiration in fatty-acid-treated AML12 cells.
Experimental context and source evidence
- evidence_access
- Primary full text
- experimental_model
- Mouse AML12 cells; 100 micromolar spermidine with palmitate/oleate for 48 hours.
- limitations
- This concentration is experimental, not a human dosing target.
- nutrient_topic
- Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Spermidine
- plain_language
- A lipid-stressed liver-cell model recovered part of its machinery.
- primary_references
- Spermidine-mediated hypusination of translation factor EIF5A improves mitochondrial fatty acid oxidation and prevents non-alcoholic steatohepatitis progression. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36057633/ · DOI 10.1038/s41467-022-32788-x
Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19) · lines 302–308
AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Mouse AML12 cells; 100 micromolar spermidine with palmitate/oleate for 48 hours. · source_derived_draft · unverified_draft
## spermidine-liver-respiration A lipid-stressed liver-cell model recovered part of its machinery. Spermidine partially restored protein synthesis and mitochondrial respiration in fatty-acid-treated AML12 cells. Model: Mouse AML12 cells; 100 micromolar spermidine with palmitate/oleate for 48 hours. Limitations: This concentration is experimental, not a human dosing target. Evidence access: Primary full text Spermidine-mediated hypusination of translation factor EIF5A improves mitochondrial fatty acid oxidation and prevents non-alcoholic steatohepatitis progression. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36057633/ · DOI 10.1038/s41467-022-32788-x
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.