{"id":"3a5e76a2-6e41-5e2b-89e8-e5cae200968c","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-liver-respiration","predicate":"restores","statement":"Spermidine partially restored protein synthesis and mitochondrial respiration in fatty-acid-treated AML12 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0c97c09f-d407-565f-b745-810045cb421a","mechanism_event_label":"A lipid-stressed liver-cell model recovered part of its machinery.","subject":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"object":{"id":"3268b0bf-3f4c-5e63-8cb6-b3b68ac69098","slug":"mouse-aml12-mitochondrial-respiration","display_name":"Mouse AML12 mitochondrial respiration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0c97c09f-d407-565f-b745-810045cb421a","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-liver-respiration-event","event_type":"observed_relationship","label":"A lipid-stressed liver-cell model recovered part of its machinery.","description":"Spermidine partially restored protein synthesis and mitochondrial respiration in fatty-acid-treated AML12 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3268b0bf-3f4c-5e63-8cb6-b3b68ac69098","slug":"mouse-aml12-mitochondrial-respiration","display_name":"Mouse AML12 mitochondrial respiration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse AML12 cells; 100 micromolar spermidine with palmitate/oleate for 48 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This concentration is experimental, not a human dosing target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A lipid-stressed liver-cell model recovered part of its machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine-mediated hypusination of translation factor EIF5A improves mitochondrial fatty acid oxidation and prevents non-alcoholic steatohepatitis progression. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36057633/ · DOI 10.1038/s41467-022-32788-x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e5c3971e-14ae-5740-99ec-66bdcec72f47","evidence_kind":"source_excerpt","locator":"Lines 302-308","start_line":302,"end_line":308,"excerpt":"## spermidine-liver-respiration\nA lipid-stressed liver-cell model recovered part of its machinery.\nSpermidine partially restored protein synthesis and mitochondrial respiration in fatty-acid-treated AML12 cells.\nModel: Mouse AML12 cells; 100 micromolar spermidine with palmitate/oleate for 48 hours.\nLimitations: This concentration is experimental, not a human dosing target.\nEvidence access: Primary full text\nSpermidine-mediated hypusination of translation factor EIF5A improves mitochondrial fatty acid oxidation and prevents non-alcoholic steatohepatitis progression. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36057633/ · DOI 10.1038/s41467-022-32788-x","model_system":"Mouse AML12 cells; 100 micromolar spermidine with palmitate/oleate for 48 hours.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}