Component

Metal-free molybdopterin / MPT

Metal-free molybdopterin / MPT. Species, exposure and limitations are retained in each linked claim.

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. The gephyrin G domain produces adenylylated molybdopterin.

    Mammalian gephyrin G domain → Metal-free molybdopterin / MPT source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/molybdenum-research/23163752.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d", "start_char": 0, "end_char": 1489, "text_sha256": "909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d"}
    experimental_model
    Purified mammalian gephyrin domains and full multidomain protein; in-vitro Moco reconstitution
    exposure
    MPT adenylation, molybdate insertion and domain-combination assays
    limitations
    No human dietary magnesium intervention; purified domain kinetics should not be turned into supplementation efficacy.
    nutrient_topic
    Molybdenum research collection; topical membership is not evidence of a direct dietary effect. · Molybdenum
    organism
    Mammalian protein system
    plain_language
    Gephyrin prepares the scaffold for metal insertion.
    primary_references
    [mo-p23163752] Metal insertion into the molybdenum cofactor: product-substrate channelling demonstrates the functional origin of domain fusion in gephyrin. (2013). https://pubmed.ncbi.nlm.nih.gov/23163752/ DOI: 10.1042/bj20121078
    tissue_or_cell_type
    Purified gephyrin

    Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17) · lines 482–493

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purified mammalian gephyrin domains and full multidomain protein; in-vitro Moco reconstitution · source_derived_draft · unverified_draft

    ### mo-gphn-adenylation The gephyrin G domain produces adenylylated molybdopterin. Condition category: normal nutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect. plain_language: Gephyrin prepares the scaffold for metal insertion. organism: Mammalian protein system tissue_or_cell_type: Purified gephyrin experimental_model: Purified mammalian gephyrin domains and full multidomain protein; in-vitro Moco reconstitution limitations: No human dietary magnesium intervention; purified domain kinetics should not be turned into supplementation efficacy. exposure: MPT adenylation, molybdate insertion and domain-combination assays evidence_span: {"source_cache": "artifacts/molybdenum-research/23163752.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d", "start_char": 0, "end_char": 1489, "text_sha256": "909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d"} [mo-p23163752] Metal insertion into the molybdenum cofactor: product-substrate channelling demonstrates the functional origin of domain fusion in gephyrin. (2013). https://pubmed.ncbi.nlm.nih.gov/23163752/ DOI: 10.1042/bj20121078
    Complete structured claim and evidence

  2. The sulfur used to form the MPT dithiolene group is supplied from the C-terminal thiocarboxylate of the small MPT-synthase subunit.

    Human MOCS2A C-terminal thiocarboxylate → Metal-free molybdopterin / MPT source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/molybdenum-research/12732628.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "154da4e4528674556bef92dfe50f098cfd9ae715c06ea01fc17d64c64b8aa319", "start_char": 0, "end_char": 1322, "text_sha256": "154da4e4528674556bef92dfe50f098cfd9ae715c06ea01fc17d64c64b8aa319"}
    experimental_model
    Purified human MPT synthase and patient-derived point mutants
    exposure
    Precursor Z/cPMP conversion assays
    limitations
    In-vitro assembly and kinetic defects; severity comparisons refer to specific patients, not a universal hierarchy.
    nutrient_topic
    Molybdenum research collection; topical membership is not evidence of a direct dietary effect. · Molybdenum
    organism
    Human proteins expressed in Escherichia coli
    plain_language
    MOCS2A carries a chemically attached sulfur supply.
    primary_references
    [mo-p12732628] Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency. (2003). https://pubmed.ncbi.nlm.nih.gov/12732628/ DOI: 10.1074/jbc.m303092200
    tissue_or_cell_type
    Reconstituted MOCS2A/MOCS2B tetramers

    Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17) · lines 404–415

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purified human MPT synthase and patient-derived point mutants · source_derived_draft · unverified_draft

    ### mo-mocs2a-sulfur The sulfur used to form the MPT dithiolene group is supplied from the C-terminal thiocarboxylate of the small MPT-synthase subunit. Condition category: normal nutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect. plain_language: MOCS2A carries a chemically attached sulfur supply. organism: Human proteins expressed in Escherichia coli tissue_or_cell_type: Reconstituted MOCS2A/MOCS2B tetramers experimental_model: Purified human MPT synthase and patient-derived point mutants limitations: In-vitro assembly and kinetic defects; severity comparisons refer to specific patients, not a universal hierarchy. exposure: Precursor Z/cPMP conversion assays evidence_span: {"source_cache": "artifacts/molybdenum-research/12732628.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "154da4e4528674556bef92dfe50f098cfd9ae715c06ea01fc17d64c64b8aa319", "start_char": 0, "end_char": 1322, "text_sha256": "154da4e4528674556bef92dfe50f098cfd9ae715c06ea01fc17d64c64b8aa319"} [mo-p12732628] Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency. (2003). https://pubmed.ncbi.nlm.nih.gov/12732628/ DOI: 10.1074/jbc.m303092200
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. Human MOCS2A and MOCS2B assemble as active MPT synthase and convert cPMP to molybdopterin.

    Human MOCS2A-MOCS2B molybdopterin synthase → Cyclic pyranopterin monophosphate / cPMP source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/molybdenum-research/12732628.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "154da4e4528674556bef92dfe50f098cfd9ae715c06ea01fc17d64c64b8aa319", "start_char": 0, "end_char": 1322, "text_sha256": "154da4e4528674556bef92dfe50f098cfd9ae715c06ea01fc17d64c64b8aa319"}
    experimental_model
    Purified human MPT synthase and patient-derived point mutants
    exposure
    Precursor Z/cPMP conversion assays
    limitations
    In-vitro assembly and kinetic defects; severity comparisons refer to specific patients, not a universal hierarchy.
    nutrient_topic
    Molybdenum research collection; topical membership is not evidence of a direct dietary effect. · Molybdenum
    organism
    Human proteins expressed in Escherichia coli
    plain_language
    Two sulfur atoms create the part of the scaffold that will hold molybdenum.
    primary_references
    [mo-p12732628] Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency. (2003). https://pubmed.ncbi.nlm.nih.gov/12732628/ DOI: 10.1074/jbc.m303092200
    tissue_or_cell_type
    Reconstituted MOCS2A/MOCS2B tetramers

    Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17) · lines 391–402

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purified human MPT synthase and patient-derived point mutants · source_derived_draft · unverified_draft

    ### mo-mpt-synthase Human MOCS2A and MOCS2B assemble as active MPT synthase and convert cPMP to molybdopterin. Condition category: normal nutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect. plain_language: Two sulfur atoms create the part of the scaffold that will hold molybdenum. organism: Human proteins expressed in Escherichia coli tissue_or_cell_type: Reconstituted MOCS2A/MOCS2B tetramers experimental_model: Purified human MPT synthase and patient-derived point mutants limitations: In-vitro assembly and kinetic defects; severity comparisons refer to specific patients, not a universal hierarchy. exposure: Precursor Z/cPMP conversion assays evidence_span: {"source_cache": "artifacts/molybdenum-research/12732628.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "154da4e4528674556bef92dfe50f098cfd9ae715c06ea01fc17d64c64b8aa319", "start_char": 0, "end_char": 1322, "text_sha256": "154da4e4528674556bef92dfe50f098cfd9ae715c06ea01fc17d64c64b8aa319"} [mo-p12732628] Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency. (2003). https://pubmed.ncbi.nlm.nih.gov/12732628/ DOI: 10.1074/jbc.m303092200
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards