{"id":"d33a0b2e-4a4c-5c87-8ad1-52a91112a91d","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-gphn-adenylation","predicate":"adenylates","statement":"The gephyrin G domain produces adenylylated molybdopterin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"38cc5df7-2f47-5a8b-aea6-3f01d976fc0c","mechanism_event_label":"Gephyrin prepares the scaffold for metal insertion.","subject":{"id":"fd48104d-1172-5b76-8a22-a2c0d9921d37","slug":"gphn-g-domain","display_name":"Mammalian gephyrin G domain","entity_type_key":"protein"},"object":{"id":"368ffa4f-299e-5783-918e-452785c0bfbd","slug":"molybdopterin","display_name":"Metal-free molybdopterin / MPT","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"38cc5df7-2f47-5a8b-aea6-3f01d976fc0c","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-gphn-adenylation-event","event_type":"biochemical_relationship","label":"Gephyrin prepares the scaffold for metal insertion.","description":"The gephyrin G domain produces adenylylated molybdopterin.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"545cec69-0265-5ab0-b1cc-58d5e7026196","slug":"mpt-amp","display_name":"Adenylylated molybdopterin / MPT-AMP","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f245ac90-f29d-57cd-a783-52793b7f07aa","slug":"gphn","display_name":"Human gephyrin / GPHN","entity_type_key":"protein"},"role":"full protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fd48104d-1172-5b76-8a22-a2c0d9921d37","slug":"gphn-g-domain","display_name":"Mammalian gephyrin G domain","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"368ffa4f-299e-5783-918e-452785c0bfbd","slug":"molybdopterin","display_name":"Metal-free molybdopterin / MPT","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/23163752.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d\", \"start_char\": 0, \"end_char\": 1489, \"text_sha256\": \"909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified mammalian gephyrin domains and full multidomain protein; in-vitro Moco reconstitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"MPT adenylation, molybdate insertion and domain-combination assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No human dietary magnesium intervention; purified domain kinetics should not be turned into supplementation efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mammalian protein system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Gephyrin prepares the scaffold for metal insertion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p23163752] Metal insertion into the molybdenum cofactor: product-substrate channelling demonstrates the functional origin of domain fusion in gephyrin. (2013). https://pubmed.ncbi.nlm.nih.gov/23163752/ DOI: 10.1042/bj20121078","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified gephyrin","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"765f06d4-035e-5b91-98f7-9d9e357914c3","evidence_kind":"source_excerpt","locator":"Lines 482-493","start_line":482,"end_line":493,"excerpt":"### mo-gphn-adenylation\nThe gephyrin G domain produces adenylylated molybdopterin.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Gephyrin prepares the scaffold for metal insertion.\norganism: Mammalian protein system\ntissue_or_cell_type: Purified gephyrin\nexperimental_model: Purified mammalian gephyrin domains and full multidomain protein; in-vitro Moco reconstitution\nlimitations: No human dietary magnesium intervention; purified domain kinetics should not be turned into supplementation efficacy.\nexposure: MPT adenylation, molybdate insertion and domain-combination assays\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/23163752.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d\", \"start_char\": 0, \"end_char\": 1489, \"text_sha256\": \"909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d\"}\n[mo-p23163752] Metal insertion into the molybdenum cofactor: product-substrate channelling demonstrates the functional origin of domain fusion in gephyrin. (2013). https://pubmed.ncbi.nlm.nih.gov/23163752/ DOI: 10.1042/bj20121078","model_system":"Purified mammalian gephyrin domains and full multidomain protein; in-vitro Moco reconstitution","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p23163752] Metal insertion into the molybdenum cofactor: product-substrate channelling demonstrates the functional origin of domain fusion in gephyrin. 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