SLC25A48-deficient human cells in the 2024 transport studies: molecular mechanisms & dependencies

How nutrients influence it

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What it does

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Recorded relationships

What it acts on

SLC25A48 loss impaired choline-derived betaine production in human-cell mitochondrial metabolism experiments.
SLC25A48-deficient human cells in the 2024 transport studies → Human mitochondrial choline-derived betaine synthesis source_derived_draftungraded
Experimental context and source evidence
availability_state
machinery_impairment Imported condition classification; unverified.
evidence_span
{"source_cache": "artifacts/choline-research/39111307.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f", "start_char": 0, "end_char": 1201, "text_sha256": "c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f"}
experimental_model
Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing
exposure
Germline mouse knockout; human-cell SLC25A48 loss; labeled choline
limitations
Transport, energy and one-carbon outcomes are model-specific; normal intake cannot be assumed to correct a carrier defect.
nutrient_topic
Choline research collection; topical membership is not evidence of a direct dietary effect. · Choline
organism
Human HEK293T cells
plain_language
A transport defect can restrict methyl-donor production upstream of betaine.
primary_references
[choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. (2024). https://pubmed.ncbi.nlm.nih.gov/39111307/ DOI: 10.1016/j.cmet.2024.07.010
tissue_or_cell_type
Brown adipose tissue and human HEK293T cells
trigger_kind
machinery_impairment Imported condition classification; unverified.
Choline: metabolism, signaling and nutrient connections (2026-09-17) · lines 490–501
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing · source_derived_draft · unverified_draft
### choline-slc25a48-betaine SLC25A48 loss impaired choline-derived betaine production in human-cell mitochondrial metabolism experiments. Condition category: machinery_impairment nutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect. plain_language: A transport defect can restrict methyl-donor production upstream of betaine. organism: Human HEK293T cells tissue_or_cell_type: Brown adipose tissue and human HEK293T cells experimental_model: Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing limitations: Transport, energy and one-carbon outcomes are model-specific; normal intake cannot be assumed to correct a carrier defect. exposure: Germline mouse knockout; human-cell SLC25A48 loss; labeled choline evidence_span: {"source_cache": "artifacts/choline-research/39111307.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f", "start_char": 0, "end_char": 1201, "text_sha256": "c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f"} [choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. (2024). https://pubmed.ncbi.nlm.nih.gov/39111307/ DOI: 10.1016/j.cmet.2024.07.010
Complete structured claim and evidence
SLC25A48 loss reduced the contribution of labeled choline to purine nucleotides in HEK293T cells.
SLC25A48-deficient human cells in the 2024 transport studies → Human HEK293T choline-derived purine nucleotide labeling source_derived_draftungraded
Experimental context and source evidence
availability_state
machinery_impairment Imported condition classification; unverified.
evidence_span
{"source_cache": "artifacts/choline-research/39111307.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f", "start_char": 0, "end_char": 1201, "text_sha256": "c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f"}
experimental_model
Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing
exposure
1 mM 13C2-choline for 24 hours in the study methods
limitations
Tracer contribution is not a claim that choline directly becomes a whole nucleotide or that dietary choline controls every purine pathway.
nutrient_topic
Choline research collection; topical membership is not evidence of a direct dietary effect. · Choline
organism
Human HEK293T cells
plain_language
Choline-derived metabolism reached nucleotide production in the tracer experiment.
primary_references
[choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. (2024). https://pubmed.ncbi.nlm.nih.gov/39111307/ DOI: 10.1016/j.cmet.2024.07.010
tissue_or_cell_type
Brown adipose tissue and human HEK293T cells
trigger_kind
machinery_impairment Imported condition classification; unverified.
Choline: metabolism, signaling and nutrient connections (2026-09-17) · lines 503–514
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing · source_derived_draft · unverified_draft
### choline-slc25a48-purines SLC25A48 loss reduced the contribution of labeled choline to purine nucleotides in HEK293T cells. Condition category: machinery_impairment nutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect. plain_language: Choline-derived metabolism reached nucleotide production in the tracer experiment. organism: Human HEK293T cells tissue_or_cell_type: Brown adipose tissue and human HEK293T cells experimental_model: Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing limitations: Tracer contribution is not a claim that choline directly becomes a whole nucleotide or that dietary choline controls every purine pathway. exposure: 1 mM 13C2-choline for 24 hours in the study methods evidence_span: {"source_cache": "artifacts/choline-research/39111307.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f", "start_char": 0, "end_char": 1201, "text_sha256": "c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f"} [choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. (2024). https://pubmed.ncbi.nlm.nih.gov/39111307/ DOI: 10.1016/j.cmet.2024.07.010
Complete structured claim and evidence

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Situations it appears in

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In the sources

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SLC25A48-deficient human cells in the 2024 transport studies